Percutaneous Autologous Bone-Marrow Grafting for Nonunions

Hernigou, Philippe; Poignard, Alexandre; Beaujean, F.; Rouard, Hélène

doi:10.2106/00004623-200507000-00003

Cited by 61 publications

(55 citation statements)

References 17 publications

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“…Therefore, investigations are needed to provide a safe and effective alternative. Tissue engineering has appeared to be a very promising technique in several regenerative applications in different anatomic regions (Fayaz et al, 2011;Hernigou et al, 2006;Jäger et al, 2011;Pacini, 2014). Indeed, the combination of cells and scaffolds could represent the optimal solution for the manage-…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have confirmed that it was important to increase the number of progenitor cells in the graft after implantation (Connolly, 1998;Hernigou et al, 2005a). Bone marrow aspirations combined with cell concentration techniques have been proposed to increase the density of progenitor cell populations (Hatzokos et al, 2011;Jacobsen et al, 2008).…”

Section: Bmac-acb Gramentioning

confidence: 98%

“…The samples were collected at the Department of Hematology. Bone marrow mesenchymal stem cells (BM-MSCs) cultures were established as previously described (Giannotti et al, 2013b;Hernigou et al, 2005a). Bone marrow aspiration concentrate (BMAC) was produced via a density gradient centrifugation using the Sorvall TM centrifuge (Thermo Scientific, MA, USA) at 3,670 rpm for 7 min.…”

Section: Isolation and Osteogenic Pre-induction Of Mscsmentioning

confidence: 99%

“…BM-MSCs aspiration from the iliac crest contains osteoprogenitor cells and has osteogenic and osteoinductive but not osteoconductive properties (Hatzokos et al, 2011). For the management of bone defects, autologous BM-MSCs have been applied together with scaffolds at the lesion site, exploiting their trophic and differentiative properties (Hernigou et al, 2005a;Kuroda et al, 2014). The need for cell attachment, proliferation, and differentiation in tissue engineering requires the design of functional scaffolds, preferably bioabsorbable.…”

Section: Bmac-acb Gramentioning

confidence: 99%

“…Because the transplanted progenitor cells compete with other cells for oxygen, the limitation of the transplanted cells is the best way to optimize progenitor cell survival that contribute to the bone formation (Hernigou et al, 2005b;Pountos et al, 2010). Hernigou et al reported that autologous bone marrow must contain at least 1500 progenitor cells per milliliter in order to be effective for the treatment of nonunion (Hernigou et al, 2005a). The mean ratio of CD34 + cells in bone marrow grafting prior injection in the study of Gangji et al achieved 1.0 ± 0.2(%) of CD34 + cells, which are precursors of hematopoietic cells (Gangji, 2004).…”

Section: Bmac-acb Gramentioning

confidence: 99%

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Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

et al. 2015

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Abstract-Autologous cancellous bone graft is currently used as a gold standard method for treatment of bone nonunion. However, there is a limit to the amount of autologous cancellous bone that can be harvested and the donor site morbidity presents a major disadvantage to autologous bone grafting. Embedding viable cells within biological scaffolds appears to be extremely promising. The purpose of this study was to assess the outcome of autologous bone marrow stem cells combined with a cancellous bone allograft as compared to an autologous bone graft in the treatment of bone nonunion. Bone marrow aspiration concentrate (BMAC) was previously produced from bone marrow aspirate via a density gradient centrifugation. Autologous cancellous bone was harvested in 9 patients and applied to the nonunion site. In 18 patients of the clinical trial group after the debridement, the bone gaps were filled with a composite of BMAC and allograft cancellous bone chips (BMAC-ACB). Bone consolidation was obtained in 88.9%, and the mean interval between the cell transplantation and union was 4.6 ± 1.5 months in the autograft group. Bone union rate was 94.4% in group of composite BMAC-ACB implantation. The time to union in BMAC-ACB grafting group was 3.3 ± 0.90 months, and led to faster healing when compared to the autograft. A mean concentration of autologous progenitorcells was found to be 2.43 ± 1.03 (x10 6 ) CD34 + cells/ml, and a mean viability of CD34 + cells was 97.97 ± 1.47 (%). This study shows that the implantation of BMAC has presented the efficacy for treatment of nonunion and may contribute an available alternative to autologous cancellous bone graft. But large clinical application of BM-MSCs requires a more appropriate and profound scientific investigations.

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Section: Discussionmentioning

confidence: 99%

Section: Bmac-acb Gramentioning

confidence: 98%

Section: Isolation and Osteogenic Pre-induction Of Mscsmentioning

confidence: 99%

Section: Bmac-acb Gramentioning

confidence: 99%

Section: Bmac-acb Gramentioning

confidence: 99%

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Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

et al. 2015

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The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

Wang

Wimpenny

Dey

et al. 2017

J Biomedical Materials Res

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There is a clear clinical need for a bioactive bone graft substitute. Poly(vinyl phosphonic acid‐co‐acrylic acid) (PVPA‐co‐AA) has been identified as a promising candidate for bone regeneration but there is little evidence to show its direct osteogenic effect on progenitor or mature cells. In this study mature osteoblast‐like cells (SaOS‐2) and human bone marrow‐derived mesenchymal stem cells (hBM‐MSCs) were cultured with PVPA‐co‐AA polymers with different VPA:AA ratio and at different concentrations in vitro. We are the first to report the direct osteogenic effect of PVPA‐co‐AA polymer on bone cells and, more importantly, this effect was dependent on VPA:AA ratio and concentration. Under the optimized conditions, PVPA‐co‐AA polymer not only has an osteoconductive effect, enhancing SaOS‐2 cell mineralization, but also has an osteoinductive effect to promote hBM‐MSCs’ osteogenic differentiation. Notably, the same PVPA‐co‐AA polymer at different concentrations could lead to differential osteogenic effects on both SaOS‐2 and hBM‐MSCs in vitro. This study furthers knowledge of the PVPA‐co‐AA polymer in osteogenic studies, which is critical when utilizing the PVPA‐co‐AA polymer for the design of novel bioactive polymeric tissue engineering scaffolds for future clinical applications. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 168–179, 2018.

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The roles of signaling pathways in bone repair and regeneration

Majidinia

Sadeghpour

Yousefi

2017

Journal Cellular Physiology

361

261

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Regenerative medicine has sparked interest in potential strategies for bone repair. Bone defects are widespread and could be caused by trauma, congenital malformations, infections, and surgery. Although bone has a large self-healing capacity, some defects or fractures are too big to regenerate. To regenerate bone structures which can be used for treatment of patients, bone growth must be induced by a number of bioactive implantable materials, cell types and intracellular, and extracellular molecular signaling pathways. Since mesenchymal stem cells (MSCs) and their differentiation during remodeling processes have important roles in bone regeneration, it is believed that understanding molecular signaling pathways involved is crucial to the development of bone implants, bone substitute materials, and cell-based scaffolds for bone regeneration. In this review, we briefly introduce concepts in fracture repair and regeneration following bone injuries, and then discuss the current clinical methods in bone regeneration. In the next section, we review the involvement of the various key signaling pathways in bone regeneration.

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Percutaneous Autologous Bone-Marrow Grafting for Nonunions

Cited by 61 publications

References 17 publications

Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

The roles of signaling pathways in bone repair and regeneration

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