Percutaneous US-Guided Implantation of Vx-2 Carcinoma into Rabbit Liver: A Comparison With Open Surgical Method

Lee, Kwang Hun; Liapi, Eleni; Buijs, Manon; Vossen, Josephina A.; Prieto-Ventura, Veronica; Syed, Labiq H.; Geschwind, Jean François H.

doi:10.1016/j.jss.2008.08.036

Cited by 25 publications

(32 citation statements)

References 9 publications

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“…However, tumor seeding occurred more often (20.8%) in the ultrasound-guided approach than in their laparotomy controls (8.3%). In addition, there was thoracoabdominal cancer cell seeding in 20.8% of cases, and also in the peritoneal cavity in 8.3% of the cases 29 . A percutaneous technique has been previously described for the inoculation of human hepatocytes in immunodeficient mice 30 .…”

Section: Introductionmentioning

confidence: 94%

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An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis

et al. 2015

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Subcutaneous xenografts have been used for decades to study hepatocellular carcinoma (HCC). These models do not reproduce the specific pathophysiological features of HCCs, which occur in cirrhotic livers that show pronounced necroinflammation, abnormal angiogenesis and extensive fibrosis. As these features are crucial for studying the role of the pathologic host microenvironment in tumor initiation, progression and treatment response, alternative HCC models are desirable. Here we describe a syngeneic orthotopic HCC model in immunocompetent mice with liver cirrhosis induced by carbon tetrachloride (CCl4) that recapitulates key features of human HCC. Induction of substantial hepatic fibrosis requires 12 weeks of CCl4 administration. Intrahepatic implantation of mouse HCC cell lines requires 30 min per mouse. Tumor growth varies by tumor cell line and mouse strain used. Alternatively, tumors can be induced in a genetically engineered mouse model. In this setting, CCl4 is administered for 12 weeks after tail-vein injection of Cre-expressing adenovirus (adeno-Cre) in Stk4−/−Stk3F/− (also known as Mst1−/−Mst2F/−; F indicates a floxed allele) mice, and it results in the development of HCC tumors (hepatocarcinogenesis) concomitantly with liver cirrhosis.

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Section: Introductionmentioning

confidence: 94%

“…Lee et al 29 have described a percutaneous ultrasound-guided approach to inoculate Vx-2 carcinoma cells in the liver of rabbits. This technique resulted in a high success rate (100%) and was less invasive than an open laparotomy method.…”

Section: Introductionmentioning

confidence: 99%

An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis

et al. 2015

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“…Each pre-anaesthetised rabbit was placed in a supine position, shaved in the subxyphoid area, and prepped in a sterile fashion following methods previously described [43,44]. Using real-time ultrasound (US) guidance (7.5-MHz linear transducer, Aloka, Wallingford, CT), percutaneous implantation of two aliquots (0.1 mlL) of VX2 tumour pieces (previously excised from the thigh of a rabbit VX2 tumour carrier) was performed via a 21-gauge angiographic catheter.…”

Section: Methodsmentioning

confidence: 99%

Image-guided thermal therapy with a dual-contrast magnetic nanoparticle formulation: A feasibility study

Attaluri

Seshadri

Mirpour

et al. 2016

International Journal of Hyperthermia

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Purpose/objective The aim of this study was to develop and investigate the properties of a magnetic iron oxide nanoparticle–ethiodised oil formulation for image-guided thermal therapy of liver cancer. Materials and methods The formulation comprises bionised nano-ferrite (BNF) nanoparticles suspended in ethiodised oil, emulsified with polysorbate 20 (BNF-lip). Nanoparticle size was measured via photon correlation spectroscopy and transmission electron microscopy. In vivo thermal therapy capability was tested in two groups of male Foxn1nu mice bearing subcutaneous HepG2 xenograft tumours. Group I (n =12) was used to screen conditions for group II (n =48). In group II, mice received one of BNF-lip (n =18), BNF alone (n =16), or PBS (n =14), followed by alternating magnetic field (AMF) hyperthermia, with either varied duration (15 or 20 min) or amplitude (0, 16, 20, or 24 kA/m). Image-guided fluoroscopic intra-arterial injection of BNF-lip was tested in New Zealand white rabbits (n =10), bearing liver VX2 tumours. The animals were subsequently imaged with CT and 3 T MRI, up to 7 days post-injection. The tumours were histopathologically evaluated for distribution of BNF-lip. Results The BNF showed larger aggregate diameters when suspended in BNF-lip, compared to clear solution. The BNF-lip formulation produced maximum tumour temperatures with AMF >20 kA/m and showed positive X-ray visibility and substantial shortening of T1 and T2 relaxation time, with sustained intratumoural retention up to 7 days post-injection. On pathology, intratumoural BNF-lip distribution correlated well with CT imaging of intratumoural BNF-lip distribution. Conclusion The BNF-lip formulation has favourable thermal and dual imaging capabilities for image-guided thermal therapy of liver cancer, suggesting further exploration for clinical applications.

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“…Anesthesia was induced with 5% isoflurane (Hospira, Warwickshire, England) and 95% oxygen (Air Gas, Salem, NH) and then sustained with 2.5% isoflurane and 97.5% oxygen at a rate of 3.5 L/min, with intravenous bolus injections of 0.25 mL propofol (10 mg/mL) (APP Pharmaceuticals, Schaumburg, Ill) via a marginal ear vein. Chunks of VX2 tumor were excised from the hind legs of the carriers, minced, and surgically implanted into the left lobe of the liver as previously described (28). The liver tumors were monitored with ultrasonography (SonoSite 180 PLUS; SonoSite, Bothell, Wash) and allowed to grow for 12-15 days after tumor implantation to reach dimensions of 2-3 cm.…”

Section: Experimental Studies: Imaging Of Ethiodized Oil-loaded Radiomentioning

confidence: 99%

Multimodality Imaging of Ethiodized Oil–loaded Radiopaque Microspheres during Transarterial Embolization of Rabbits with VX2 Liver Tumors

Tacher¹,

Durán²,

Lin³

et al. 2016

Radiology

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equally to this work.q RSNA, 2015 Purpose:To assess the visibility of radiopaque microspheres during transarterial embolization (TAE) in the VX2 rabbit liver tumor model by using multimodality imaging, including singlesnapshot radiography, cone-beam computed tomography (CT), multidetector CT, and micro-CT. Materials and Methods:The study was approved by the institutional animal care and use committee. Fifteen VX2-tumor-bearing rabbits were assigned to three groups depending on the type of embolic agent injected: 70-150-mm radiopaque microspheres in saline (radiopaque microsphere group), 70-150-mm radiopaque microspheres in contrast material (radiopaque microsphere plus contrast material group), and 70-150-mm radiolucent microspheres in contrast material (nonradiopaque microsphere plus contrast material group). Rabbits were imaged with singlesnapshot radiography, cone-beam CT, and multidetector CT. Three to 5 weeks after sacrifice, excised livers were imaged with micro-CT and histologic analysis was performed. The visibility of the embolic agent was assessed with all modalities before and after embolization by using a qualitative three-point scale score reading study and a quantitative assessment of the signal-to-noise ratio (SNR) change in various regions of interest, including the tumor and its feeding arteries. The Kruskal-Wallis test was used to compare the rabbit characteristics across groups, and the Wilcoxon signed rank test was used to compare SNR measurements before and after embolization. Results:Radiopaque microspheres were qualitatively visualized within tumor feeding arteries and targeted tissue with all imaging modalities (P , .05), and their presence was confirmed with histologic examination. SNRs of radiopaque microsphere deposition increased after TAE on multidetector CT, cone-beam CT, and micro-CT images (P , .05). Similar results were obtained when contrast material was added to radiopaque microspheres, except for additional image attenuation due to tumor enhancement. For the group with nonradiopaque microspheres and contrast material, retained tumoral contrast remained qualitatively visible with all modalities except for micro-CT, which demonstrated soluble contrast material washout over time. Conclusion:Radiopaque microspheres were visible with all imaging modalities and helped increase conspicuity of the tumor as well as its feeding arteries after TAE in a rabbit VX2 liver tumor model.q RSNA, 2015

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Percutaneous US-Guided Implantation of Vx-2 Carcinoma into Rabbit Liver: A Comparison With Open Surgical Method

Cited by 25 publications

References 9 publications

An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis

An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis

Image-guided thermal therapy with a dual-contrast magnetic nanoparticle formulation: A feasibility study

Multimodality Imaging of Ethiodized Oil–loaded Radiopaque Microspheres during Transarterial Embolization of Rabbits with VX2 Liver Tumors

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