This study aims to validate the Burnout Assessment Tool (BAT) adapted to the Italian education sector. Teacher burnout is physical and emotional pain, due to prolonged exposure to school-related stress factors. Previous research has abundantly proven that preventive assessment of teachers’ risk level for burnout may reduce adverse outcomes. In this regard, new assessment tools, able to bring together evidence from fifty years of research on this topic, were mainly used to monitor burnout-risk levels in the school context. For the present work, 846 Italian teachers (Female, 91.1%; M age = 47.52; SD = 9.94) were involved in the study. Confirmatory factor analysis supported a four-factor structure for the core dimensions (BAT-C; exhaustion, mental distance, emotional impairment, cognitive impairment), and a two-factor structure for the secondary dimensions (BAT-S; psychological distress, psychosomatic complaints). The Italian version of the BAT-C and BAT-S has shown good internal consistency (respectively, α = 0.900 and ω = 0.913; α = 0.845 and ω = 0.857) and validity (all correlations between variables showed a p value < 0.01). Our findings support the Italian adaptation of the original version of the BAT as a valid instrument for measuring teachers’ burnout through principal and secondary symptoms.