Perfil enzimático de α-amilase, lipase e tripsina do pâncreas e crescimento do fígado, intestino e pâncreas de frangos de corte na fase de 1 a 21 dias de idade

Abstract

Palavras-chave: enzimas digestivas, desenvolvimento enzimático, peso do aparelho digestório Enzymatic profile of α α α α α-amylase, lipase and trypsin in the pancreas and the growth of the liver, intestine and pancreas in broiler chicks from one to 21 days of age 1ABSTRACT -With the objective of assessing the development of organs of the digestive tract and the enzymatic profile α-amylase, lipase and trypsin, an experiment lasting 21 days was carried out using one-day-old male Avian Farm broiler chicks, reared in heated batteries and fed ad libitum with water and purified diets containing all L-essential amino acids, glutamic acid (nonspecific nitrogen source), minerals and vitamins. A randomized complete design was used with four replications of ten birds. At one, seven, 14 e 21 days of age, four chicks were sacrificed and the liver, pancreas and intestine were removed and weighed to determine the pancreas a-amylase, lipase e trypsin activities. The absolute weights increased with age. The pancreas and intestine relative weight reduced at 21 days of age. Specific activities (UI/mg protein) of α-amylase and lipase were higher on the 1 st day, decreased on the 7 th day, reaching the maximum peak on the 14 th day decreasing again on the 21 st day. Trypsin specific activity (UI/mg protein) did not vary with age. The activities per 100 g of body weight of α-amylase, lipase and trypsin (UI/100g body weight) increased from the first to the 14 th day of age and showed a reduction on the 21 st day. The maximum activities of the digestive enzymes α-amylase and lipase occurred on the 14 th day of age. The enzymatic profile of trypsin in general did not alter with age, that can be explained by the fact that the animals were raised on purified diets, that did not stimulate proteolytic enzymes.

<b>Performance, gut morphometry and enzymatic activity of broilers fed neonatal supplementation in housing

“…Lipase activity was also influenced by the neonatal supplement (p < 0.05), and the non-parametric Kruskal-Wallis test was used to evaluate the dataStudies indicate that the activity of amylase and lipase oscillates during the life of the bird, and the peak can be reached at eight days of age (Maiorka, Dahlke, & Morgulis, 2006 The higher activity of pancreatic enzymes is related to the higher relative weight of the pancreas, so the presence of substrate and enzymatic activity are due to maturation of the pancreas (Moraes et al, 2008). The specific activity of lipase presented a distinct behavior when compared to the specific activity of amylase, because through the results obtained here the lipase activity was low.…”

Nucleotídeos purificados em rações para reprodutores e proles regulam a atividade de enzimas digestivas e a morfologia intestinal em tilápia-do-Nilo

“…This enzyme's function is to digest ingested proteins, which is crucial during the initial phase when protein demands are higher. Its reaction is characterized by the participation of a serine residue in the active center and catalyzes the hydrolysis of peptide bonds (Moraes et al, 2009). The increase in trypsin can be explained by the improvement of enzymatic activities in the intestinal mucosa, which activates trypsinogen.…”