The objective of this work was to evaluate the combined effects of air temperature and air velocity on the behavior of Japanese quail (Coturnix coturnix japonica). A total of 216 Japanese quail in their initial laying phase were used. Bird behavior was categorized with an ethogram (eat, drink, stop, open wings/shiver, others). The experimental design was a randomized complete block, in a 4x4 factorial arrangement, with four air velocities (0, 1, 2, and 3 m s-1) and air temperatures (17, 23, 29, and 35°C). The behavior “stop” was greater when the birds were subjected to 17°C. At 35°C, a significant reduction (p<0.05) was observed in the behavior “eat” at 0 m s-1, compared with the other velocities. The behaviors of laying quail are similar in the morning and in the afternoon. Quail remain stopped for a longer time under cold stress conditions, at 17°C.