This study aims to determine the effect of vitamin E and selenium injections on the semen quality of male Bangkok chickens. The study used 18 male Bangkok chickens aged 1.5 years from April 10, 2023, to May 26, 2023, consisting of 3 treatments of 6 repeats using Complete Randomized Design, P0 (without injection of vitamin E and selenium), P1 (0.6 ml/head), P2 (0.9 ml/head). Chickens are kept in individual cages and given feed for corn, bran, and concentrates. Chickens are injected once every 2 weeks for 6 weeks. Data analysis used Analysis of Variance (ANOVA), descriptive analysis, and Kruskal Wallis Test followed by Duncans Multiple Ring Test and Mann-Whitney test. The result of observing the color of the injection treatment on Bangkok chickens was milky white, the smell was specific with medium thickness. pH, mass movement, and spermatozoa abnormalities were not significantly different in treatments P0, P1, and P2, the volume of semen obtained was significantly different in treatments P0, P1, and P2, the result of the mean sperm concentration in treatments P0 and P1 showed the significantly different result, however, P0 and P2 with P1 and P2 show no significant difference. It was concluded that the injection of vitamin E and selenium 0.6 ml/head had a better effect on volume and concentration than without injection and injection 0.9 ml/head.