The goal of this study was to analyze the genetic and environmental factors of selected meat yield indicators in Fleckvieh cattle in the Czech Republic, through the application of station (S) and field (F) testing methods. Data collected from fattened bulls were analyzed for F (n = 9378) and for S (n = 6346). In the F method and the S method, the values of the main meat yield indicators were as follows: carcass weight 402.91 kg (F), 339.37 kg (S); carcass daily gain 626.05 g/day (F), 609.74 g/day (S); SEUROP carcass classification 2.73 (F), 2.19 (S). Environmental factors were found to have a significant impact on the selected meat yield indicators; their heritability ranged from 0.14 (SEUROP classification) to 0.33 (dressing percentage). The genetic trend was significantly positive only in relation to those meat yield traits, which had a positive link to the size or weight of the animal. The genetic correlation between observations obtained in the S and F methods of testing was very high in relation to the carcass daily gain (0.8351) and carcass weight (0.8244), while slightly lower correlations were calculated for the SEUROP classification. A genetic evaluation of the degree of fatness is not routinely performed in Fleckvieh populations, and the newly established heritability for this trait ranges between 0.17–0.20. The genetic correlation between beef yield indicators and the exterior trait of muscularity was also established, and shows a strong link to the net daily gain, the SEUROP classification and body weight (0.79–0.97). The aim of the study was to evaluate the genetic and environmental effects on meat yield and also estimate genetic parameters for new traits. We can also state, based on the results, that a strong positive genetic trend is confirmed, especially in traits related to the size or weight of animals. This result can be used in breeding programs of dual-purpose cattle, where we can genetically improve the meat and milk yield through the body size.