This experimental study investigates the performance, combustion, and emission characteristics of a common rail direct injection diesel engine running on used cooking biodiesel prepared through transesterification. The nanoparticles calcium oxide (CaO) and alumina (Al2O3) are mixed with the B20 blend using an ultrasonicator to create a homogeneous mixture. The following five fuel series are used in the study: a B20 blend with three different nanoparticle ppms are blended to form Al2O3CaO 90 ppm, Al2O3 + CaO 60 ppm, and Al2O3 + CaO 30 ppm. The enhanced surface area to volume ratio of nanoparticles enhances the degree of mixing and chemical reactivity during combustion. Also, the characteristics of diesel engines result in reduced specific fuel consumption. The test fuel B20 + 90 ppm (90 ppm nanoparticles dispersion) results 9% and 11% increase in brake thermal efficiency compared to diesel and B20, respectively. The presence of nanoparticles in biodiesel has initiated the combustion slightly earlier due to its improved ignition qualities that permit catalytic combustion and a shorter ignition delay. In comparison to diesel and B20, the Al2O3CaO90 ppm fuel blend lowers NOx emissions by 18% and 21%, carbon monoxide emissions by 40% and 43%, hydrocarbon emissions by 35% and 40%, and smoke emissions by 24% and 29%, respectively.