In this paper, the optimal refrigerant mass charge amount of R417A, as a substitute for R22, was experimentally investigated in a split-type air-conditioner with no changes in system configuration. Experiments were conducted for both refrigerants over a range of mass charge from 500 to 850 g in a 12,000 Btuh À1 split air-conditioner as the case study which is a conventional refrigeration system in residential sector of Iran. In order to study the effect of refrigerant mass charge on performance of refrigeration cycle, energy and exergy analysis were performed to determine cooling capacity, energy efficiency ratio (EER) and exergy efficiency.Results showed that the maximum EER for R22 and R417A are 2.48 and 2.41, and the maximum exergy efficiencies obtained for R22 and R417A are around 7.3% and 7%, respectively. Based on both EER and exergy efficiency, the optimum mass charge for R22 and R417A refrigerants are around 640 g and 650 g, respectively. In addition, it was found that under optimal charge conditions, the cooling capacity, EER and exergy efficiency of the system while working with R417A are lower than R22, just by 2.5%, 3%, and 4.5% respectively. Comparison results indicate that R417A can be considered as a suitable substitute for R22, which can almost provide the same performance without need to change the system components.