Performance assessment of the two oxygen sensor based respirometric platforms with complex media and in selective bacterial assays

Elisseeva, Sophia; Santovito, Elisa; Linehan, Eva; Kerry, Joe P.

doi:10.1016/j.snb.2023.133582

Cited by 8 publications

(26 citation statements)

References 32 publications

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“…They are better suited for use in high throughput cell-based screening assays of small samples on standard bioassay substrates and equipment (i.e., microrespirometry). However, liquid probes have their own challenges, such as (i) being poorly usable in large samples (costly); (ii) being more susceptible to optical and quenching interferences than 'shielded' solid-state sensors and nanoparticles [69]; (iii) unwanted interactions with cells and surfaces during the assay (e.g., non-specific binding); (iv) long-term storage in liquid form or drying/reconstitution issues and (v) complex synthesis.…”

Section: O 2 Sensing Materialsmentioning

confidence: 99%

“…Of note, low O 2 levels in the sample in Phases 2/3 may trigger other processes, such as (i) inhibition of respiration, i.e., a reduction of v o ; (ii) anoxic cell death reducing the number of respiring cells, N, and (iii) increased O 2 back-diffusion due to a higher gradient. These factors can alter the classical sigmoidal shape and produce more complex respiration profiles [69]. However, the initial part of Phase 2 of the profile for which dissolved O 2 levels still remain close to ambient O 2 is usually stable and less affected by such factors.…”

Section: Mammalian Cell Respirationmentioning

confidence: 99%

“…Additional equipment includes just media and a pipette/dispenser for Platform 6, a simple incubator or block heater for Platforms 7-9 and a heat-sealing machine for Platform 9. Detection equipment for O 2 respirometry is mostly LT based, which provides stable and accurate measurement of sensor signals and resilience to sample matrix effects and optical interferences that may occur in TVC assays [69]. These features are also aided by the use of indicator dyes with high brightness and longwave spectral characteristics, such as PtBP used in Platforms 3, 6-9 and 11.…”

Section: Existing Formats and Applications Of Bacterial Respirometrymentioning

confidence: 99%

“…Conversely, certain chemical ingredients present in complex and even regular growth media may affect the sensor/probe signals [87]. Thus, several pH indicators, chromogenic substrates, metal ions, surfactants and aromatics present in common selective media were shown to cause optical and quenching interferences [69]. Such effects, if seen, should be tackled by optimizing the sensor material and assay settings.…”

mentioning

confidence: 99%

“…Such effects, if seen, should be tackled by optimizing the sensor material and assay settings. In this regard, 'shielded' solid-state sensors look more favorable than soluble probes [69].…”

mentioning

confidence: 99%

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Oxygen Sensor-Based Respirometry and the Landscape of Microbial Testing Methods as Applicable to Food and Beverage Matrices

Papkovsky

Kerry

2023

Sensors

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The current status of microbiological testing methods for the determination of viable bacteria in complex sample matrices, such as food samples, is the focus of this review. Established methods for the enumeration of microorganisms, particularly, the ‘gold standard’ agar plating method for the determination of total aerobic viable counts (TVC), bioluminescent detection of total ATP, selective molecular methods (immunoassays, DNA/RNA amplification, sequencing) and instrumental methods (flow cytometry, Raman spectroscopy, mass spectrometry, calorimetry), are analyzed and compared with emerging oxygen sensor-based respirometry techniques. The basic principles of optical O2 sensing and respirometry and the primary materials, detection modes and assay formats employed are described. The existing platforms for bacterial cell respirometry are then described, and examples of particular assays are provided, including the use of rapid TVC tests of food samples and swabs, the toxicological screening and profiling of cells and antimicrobial sterility testing. Overall, O2 sensor-based respirometry and TVC assays have high application potential in the food industry and related areas. They detect viable bacteria via their growth and respiration; the assay is fast (time to result is 2–8 h and dependent on TVC load), operates with complex samples (crude homogenates of food samples) in a simple mix-and-measure format, has low set-up and instrumentation costs and is inexpensive and portable.

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Section: O 2 Sensing Materialsmentioning

confidence: 99%

Section: Mammalian Cell Respirationmentioning

confidence: 99%

Section: Existing Formats and Applications Of Bacterial Respirometrymentioning

confidence: 99%

mentioning

confidence: 99%

“…Such effects, if seen, should be tackled by optimizing the sensor material and assay settings. In this regard, 'shielded' solid-state sensors look more favorable than soluble probes [69].…”

mentioning

confidence: 99%

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