Performance characteristics of a real-time direct deposition gas chromatography/Fourier transform infrared spectrometry system

Bourne, Sidney.; Haefner, Andrew M.; Norton, Kelly L.; Griffiths, Peter R.

doi:10.1021/ac00221a007

Cited by 89 publications

(24 citation statements)

References 11 publications

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“…An FTS-60A FT-IR spectrometer (Bio-Rad, Digilab Division, Cambridge, MA), equipped with a direct deposition Tracer TM interface operating near 77K under vacuum, was used. This system has been described in detail (11). (B) Separation of same mixture of FAME by silver ion-high-performance liquid chromatography (HPLC).…”

Section: Methodsmentioning

confidence: 99%

“…Several fractions, including the one containing the trans monounsaturated C 18 FAME, were isolated by Ag + -HPLC. The double bond configuration for the FAME in the trans 18:1 HPLC fraction was confirmed by using GC-direct deposition-Fourier transform infrared (DD-FTIR) spectroscopy (11). A portion of this HPLC fraction was converted to the 2-alkenyl-4,4-dimethyloxazoline (DMOX) derivative (12,13) and used to confirm the double bond position for individual trans 18:1 FAME positional isomers by GC-electron ionization mass spectrometry (EIMS).…”

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confidence: 99%

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Spectral confirmation of trans monounsaturated C₁₈ fatty acid positional isomers

Mossoba

McDonald²,

Roach

et al. 1997

J Americ Oil Chem Soc

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The trans octadecenoic acid methyl ester isomers were obtained from a partially hydrogenated soybean oil sample and isolated by silver-ion high-performance liquid chromatography. The double bond configuration was confirmed to be trans by using gas chromatography-direct deposition-Fourier transform infrared spectroscopy. The double bond positions for nine individual trans octadecenoic acid positional isomers were confirmed by gas chromatography-electron ionization mass spectrometry after derivatization to 2-alkenyl-4,4-dimethyloxazoline. These nine trans positional isomers were resolved on either one of the two polar 100% cyanopropylpolysiloxane 100-m capillary columns, SP 2560 and Cp-Sil 88, at an isothermal temperature of 140°C. These nine isomers were confirmed to have double bonds at carbons C-8 through C-16. The pair of trans octadecenoic acid positional isomers with double bonds at C-13 and C-14 are reported for the first time to be resolved by gas chromatography. JAOCS 74, 125-130 (1997).KEY WORDS: 4,4-Dimethyl oxazoline (DMOX), direct deposition, trans double bonds, fatty acid methyl esters (FAME), gas chromatography, infrared spectroscopy, mass spectrometry, monounsaturated fatty acids, positional isomers. FIG. 4. GC-EIMS spectra observed for the ∆8, ∆9, and ∆10 trans 18:1 DMOX positional isomers; a gap of 12 mass units was observed between the pairs of adjacent ions at m/z 182 and 194; 196 and 208; and 210 and 222, respectively; the ions due to allylic cleavage were found at m/z 168, 222, and 236; 182, 236, and 250; and 196, 250, and 264, respectively. See Figure 3 for abbreviations. 130 M.M. MOSSOBA ET AL. JAOCS, Vol. 74, no. 2 (1997) FIG. 6. GC-EIMS spectra for ∆6 and ∆7 trans 18:1 DMOX positional isomer standards. The inset shows the m/z 113 chromatographic trace observed for a co-injected qualitative test mixture of these two standards. See Figure 3 for abbreviations.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Spectral confirmation of trans monounsaturated C₁₈ fatty acid positional isomers

Mossoba

McDonald²,

Roach

et al. 1997

J Americ Oil Chem Soc

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“…[1617] A unified interface for GC, HPLC, and SFC hyphenation to FTIR applying IR microscopic technique is also available today. [18]…”

Section: Available Hyphenated Techniquesmentioning

confidence: 99%

Introduction to hyphenated techniques and their applications in pharmacy

Patel¹,

Patel²,

Patel³

et al. 2010

Pharm Methods

115

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The hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopic detection technology. The remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products. In this article, recent advances in the applications of various hyphenated techniques, e.g., GC-MS, LC-MS, LC-FTIR, LC-NMR, CE-MS, etc. in the context of pre-isolation analyses of crude extracts or fraction from various natural sources, isolation and on-line detection of natural products, chemotaxonomic studies, chemical fingerprinting, quality control of herbal products, dereplication of natural products, and metabolomic studies are discussed with appropriate examples.

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“…When the composition of the GC-eluant is altered, along with the retention time, the changes in the frequency of the sound produced can be detected using a microphone. After a Fourier transform, 26-31 a very sharp frequency peak can be observed. In terms of sensitivity, this method is superior to that for a typical thermal conductivity detector, and can be very useful in the analysis of gases, irrespective of whether they are organic, inorganic or rare gases.…”

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confidence: 99%

The Use of a Milli-whistle as a Detector in Gas Analysis by Gas Chromatography

Lin

et al. 2014

ANAL. SCI.

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Performance characteristics of a real-time direct deposition gas chromatography/Fourier transform infrared spectrometry system

Cited by 89 publications

References 11 publications

Spectral confirmation of trans monounsaturated C₁₈ fatty acid positional isomers

Spectral confirmation of trans monounsaturated C₁₈ fatty acid positional isomers

Introduction to hyphenated techniques and their applications in pharmacy

The Use of a Milli-whistle as a Detector in Gas Analysis by Gas Chromatography

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Performance characteristics of a real-time direct deposition gas chromatography/Fourier transform infrared spectrometry system

Cited by 89 publications

References 11 publications

Spectral confirmation of trans monounsaturated C18 fatty acid positional isomers

Spectral confirmation of trans monounsaturated C18 fatty acid positional isomers

Introduction to hyphenated techniques and their applications in pharmacy

The Use of a Milli-whistle as a Detector in Gas Analysis by Gas Chromatography

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Spectral confirmation of trans monounsaturated C₁₈ fatty acid positional isomers

Spectral confirmation of trans monounsaturated C₁₈ fatty acid positional isomers