Performance comparison of three methods for detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in drinking-water treatment sludge

Giglio, Guilherme Lelis; Sabogal-Paz, Lyda Patrícia

doi:10.1007/s10661-018-7057-9

Cited by 15 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies in Brazil utilizing the CCF approach combined with immunomagnetic separation (IMS) have shown slightly improved recovery rates. Giglio and Sabogal-Paz (2018) assessed sludge generated from a water treatment plant and reported an average recovery of 46.1% for Giardia spp. cysts.…”

Section: Resultsmentioning

confidence: 99%

Dairy wastewater treatment employing microencapsulated Pseudomonas aeruginosa on low acyl gellan gum

Cuello,

Alcázar,

Castellanos

2024

Rev. ambiente água

View full text Add to dashboard Cite

This study assessed the ability of Pseudomonas aeruginosa, microencapsulated in gellan gum, to decontaminate dairy wastewater and explored the potential reuse of microcapsules. P. aeruginosa was microencapsulated using the internal ionic gelation technique, employing low-acyl gellan gum as the wall material. The free and microencapsulated P. aeruginosa were inoculated into 150 mL of sterile wastewater and incubated in a shaking flask (150 rpm) at 30°C. Subsequently, the Baranyi Model was employed to calculate the growth parameters of P. aeruginosa. Concurrently, Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) were determined. The obtained results indicated that the microencapsulation process reduced the growth rate of the encapsulated microorganism. However, the microencapsulated bacteria achieved COD and BOD reduction percentages of 61.54% and 64.05%, respectively. Similarly, when reusing the microcapsules, removal percentages exceeding 57.00% were achieved. These findings could have significant implications for the industry in terms of reducing effluent contamination caused by substantial amounts of pollutants. Keywords: dairy wastewater, gellan gum, microencapsulation, Pseudomonas aeruginosa.

show abstract

Section: Resultsmentioning

confidence: 99%

Dairy wastewater treatment employing microencapsulated Pseudomonas aeruginosa on low acyl gellan gum

Cuello,

Alcázar,

Castellanos

2024

Rev. ambiente água

View full text Add to dashboard Cite

show abstract

“…It is noted that IFA refers to the current protocol established by USEPA methods 1623.1 and 1693 for analysis of water and disinfected wastewater (2012,2014), thus it is assumed to be the first resource WASH practitioners would rely on for periodically monitoring quality considering protozoan parasites. In fact, several studies on environmental samples that do not have a standardised detection method include IFA (Olson et al 1999;Greinert et al 2004;Graczyk et al 2008;Grit et al 2012;Giglio & Sabogal-Paz 2018;Sammarro-Silva & Sabogal-Paz 2020, 2021a, 2021bOgura & Sabogal-Paz 2021. The motive for choosing this protocol is assumed to be its high sensitivity, as older references that consider complex matrices were also immunofluorescencebased, as in Olson et al (1999), which used IFA to quantify the survival of cysts seeded into soil and cattle waste.…”

Section: Immunofluorescence Assay and Dapi Stainingmentioning

confidence: 99%

Exploring challenges in Giardia cyst visualisation by common microscopy methods

Fava

Silva

Freitas

et al. 2023

Water Practice and Technology

Self Cite

View full text Add to dashboard Cite

Giardia spp. is an intestinal parasite responsible for worldwide disease outbreaks. Guiding researchers and practitioners to choose among current methods for microscopy detection of the infectious forms may be directly beneficial to public health and the environment. This study provides an overall comparison of brightfield (BF), fluorescence and darkfield (DF) microscopies for detecting Giardia duodenalis and Giardia muris cysts, by illustrating micrographs of such protocols applied to purified samples, as well as discussing advantages and constraints based on secondary information and collected data. BF analysis included Lugol's iodine staining. In fluorescence microscopy, samples were processed by immunofluorescence assay (IFA) with DAPI and by standalone DAPI dye. Cyst suspensions were also analysed by DF microscopy using a recently developed low-cost system. The three techniques enabled detecting Giardia spp. cysts, although they did not provide species identification by morphology. The overview of each method points out some relevant aspects to consider when selecting common optical microscopy techniques, and includes challenges and advantages regarding each of them.

show abstract

“…In this sense, studies that focus on the microbiological composition of WTP sludge, as well as its pathogenicity, are extremely necessary, especially related to environmental regulations, since the microbiological characterization of waste from WTPs can encourage new policies related to their management, where the absence of WTS-specific guidelines currently allows reuse of the WTS without any restrictions (Ahmad et al, 2017;Giglio and Sabogal-Paz, 2018;Bernegossi et al, 2022). Several studies address the microbiological diversity present in WTP sludge, showing the importance of microbiological characterization, especially related to close contact with workers, which can represent a risk of infection (Makovcova et al, 2015;Giglio and Sabogal-Paz , 2018;Xu et al, 2018;Ullmann et al, 2019;Ranković et al, 2020;Wang et al, 2021;Bernegossi et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Microbiological composition of sludge generated in water treatment plants

Leite,

Martins,

Rodgher

et al. 2023

Rev. ambiente água

View full text Add to dashboard Cite

Studies that focus on the microbiological composition of water treatment plants (WTP) sludge, as well as its pathogenicity, are extremely necessary, especially with regard to environmental regulations, where the microbiological characterization of WTP waste can encourage new policies related to its management. In Brazil, few studies address WTP sludge, which, in general, is classified as non-hazardous and non-inert waste, with the microbiological characterization of this material being little explored. This case study performed the microbiological characterization of sludge samples from two WTPs located in the state of São Paulo, before and after the centrifugation process. The determination of microbial density and morphology, Gram staining, and the identification of the presence of total and thermotolerant coliforms were performed with samples produced in two different years, in WTPs that used different coagulants (liquid aluminum sulfate or polyaluminum chloride and ferric chloride). Results were evaluated along with the physicochemical analysis of the composition of this waste. The presence of microalgae and protozoa in non-centrifuged WTP sludge and the presence of total and thermotolerant coliforms in WTP sludge before and after centrifugation are among the main results of this study. Keywords: beneficial use, microbiological composition, water treatment plant sludge.

show abstract

Performance comparison of three methods for detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in drinking-water treatment sludge

Cited by 15 publications

References 22 publications

Dairy wastewater treatment employing microencapsulated Pseudomonas aeruginosa on low acyl gellan gum

Dairy wastewater treatment employing microencapsulated Pseudomonas aeruginosa on low acyl gellan gum

Exploring challenges in Giardia cyst visualisation by common microscopy methods

Microbiological composition of sludge generated in water treatment plants

Contact Info

Product

Resources

About