Performance comparison of two analytical methods for the detection of tissues of the central nervous system in sausages: results of an interlaboratory study

Agazzi, Marie-Elisabeth; Barrero-Moreno, Josefa; Lücker, Ernst; Holst, Christoph von; Anklam, Elke

doi:10.1007/s00217-002-0558-2

Cited by 27 publications

(16 citation statements)

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“…They noted that the GFAP assay was easier to use and to interpret the data than the NSE assay. In a similar study by Agazzi et al [138], the sensitivity of the NSE test kit was reported as being 0.5% (g/g) CNS tissue in raw and medium-heated (80 °C for 20 min) meat samples, rising to 2.0% (g/g) in strongly heated (120 °C for 20 min) meat materials. Another study [146] also found that the NSE immunoreactivity in samples of pure brain and brain muscle dropped signifi-cantly with time at 100 °C, almost disappearing after a 2 h heat treatment.…”

Section: Neuron-specific Enolase (Nse)mentioning

confidence: 68%

“…PCR methods are also prone to contamination. [130][131][132] Immunochemical detection methods based on the specific immunoreactions between an antibody and its target antigen have been extensively employed for the detection of CNS contamination in meat (Table 1), generally in the form of an enzyme-linked immunosorbent assay (ELISA), [76,128,[133][134][135][136][137][138][139][140][141][142][143] immunoblotting [137][138][139][140][144][145][146][147][148][149] or immunohistochemistry. [145,146,[150][151][152] There are a number of advantages associated with using an immunoassay.…”

Section: Detection Methods For Surveillance Of Prion Diseasesmentioning

confidence: 99%

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Spread and Control of Prion Diseases in the Food and Feed Chains

Rao¹,

Hsieh²

2016

Significance, Prevention and Control of Food Related Diseases

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Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a group of chronic, progressive, and fatal neurodegenerative disorders that affect a variety of mammalian species. This chapter discusses the issues raised by two foodborne prion diseases, namely bovine spongiform encephalopathy (BSE) and variant Creutzfeldt-Jakob disease (vCJD), particularly those related to their spread in cattle and humans, the contamination of specified risk material (SRM) in meat, the relevant regulations, and appropriate detection methods for surveillance.

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Section: Neuron-specific Enolase (Nse)mentioning

confidence: 68%

Section: Detection Methods For Surveillance Of Prion Diseasesmentioning

confidence: 99%

Spread and Control of Prion Diseases in the Food and Feed Chains

Rao¹,

Hsieh²

2016

Significance, Prevention and Control of Food Related Diseases

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“…Under certain pre-treatment conditions proteins are denatured and antigenicity is decreased or will be lost. In raw or only moderately heated sausages GFAP and NSE were detected at a limit of 0.5% CNS tissue [23]. For strongly heated meat the limit decreased to 2% for NSE but remained constant for GFAP determined with single commercial test kits [23].…”

Section: Resultsmentioning

confidence: 95%

Immunological one‐step determination of the central nervous system indicator proteins, neuron‐specific enolase and glial fibrillary acidic protein, in meat products

Kuczius

Böhler

Karch

2010

Molecular Nutrition Food Res

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The determination of specific marker proteins is important in the prevention of infections and transmission of disease. Several diagnostic assays have been developed but these are mostly restricted to the detection of single antigens. Thus there is a need for multiplex detection assays for the simultaneous analysis of several disease indicators. Consumer protection against the transmission of prion diseases is ensured by the removal of specified risk material from cattle meat during slaughtering and this is regulated by law. The investigation reported here describes a one-step determination on immunoblots of the simultaneous detection of two indicator proteins, neuron-specific enolase and glial fibrillary acidic protein, in tissues of the central nervous system. Although the two proteins run in polyacrylamide gels with similar molecular masses the indicators are differentiated by immunological reactions followed by visualizing the different coloured specific protein bands which develop. The enolase exhibits a brown band, whereas the glial fibrillary acidic protein is red. Immunoblotting has proved to be a suitable assay for multiplex analysis of marker proteins possessing similar molecular weights and is therefore a suitable tool for application in food, veterinary and medical facilities.

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“…In one study the NSE test was more consistent in detecting the presence of CNS tissues below the 1% level, but the GFAP-ELISA kit was technically easier to use and the data simpler to interpret (Hughson et al, 2003). In two other studies the GFAP test kit was more sensitive, easier, faster to run and less expensive than the NSE kit (Agazzi et al, 2002;Hajmeer et al, 2003). However, it was demonstrated that this commercially available GFAP-ELISA would produce false-positive results in samples with high blood content (Schurr et al, 2004).…”

Section: Of 25mentioning

confidence: 99%

Opinion of the Scientific Panel on biological hazards (BIOHAZ) on BSE risk from dissemination of brain particles in blood and carcass following stunning

2004

EFSA Journal

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SUMMARYThe European Commission (EC) requested the European Food Safety Authority (EFSA) and its Scientific Panel on Biological Hazards for an update of the latest SSC opinion of 2002 related to the risk of dissemination of brain particles into the blood and carcass when applying certain stunning methods.The Scientific Panel on Biological Hazards concludes that brain damage caused by both penetrating and non-penetrating captive bolt stunning in cattle, as well as that caused by penetrating captive bolt stunning in sheep, can result in occurrence of Central Nervous System (CNS) tissue emboli in venous blood draining the head. Therefore, the nonpenetrating method currently used cannot be considered as an alternative method to the penetrating method. It also concluded that following penetrating captive bolt stunning, the likelihood of CNS tissue embolism is higher in sheep than in cattle. New data, based on the results of detection methods currently available, confirm the occurrence of CNS embolism following penetrating or non-penetrating stunning methods. However, methods for examination of different tissue/organs for the presence and quantification of the CNS material have not been optimised. It was also concluded that analysis of the available data can not confirm either an association, or the absence of an association, of CNS tissue embolism with a number of alternative methods, including electrical stunning method.The Scientific Panel on Biological Hazards recommends that further studies in particular on modifications of the current captive bolt stunning methods so as to prevent CNS embolism are required. Alternatively, the replacement of these methods should be investigated. In addition, also validation studies on the occurrence of stunning-associated CNS embolism conducted under commercial conditions are required. Such studies should focus on the involvement of systemic arterial circulation in the distribution of CNS emboli. Research should also focus on optimized methods for the detection and quantification of stunning-induced dissemination of CNS material to different organs/tissues and on optimal sampling methods/plans, which can provide comparable results between various studies.

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Performance comparison of two analytical methods for the detection of tissues of the central nervous system in sausages: results of an interlaboratory study

Cited by 27 publications

References 0 publications

Spread and Control of Prion Diseases in the Food and Feed Chains

Spread and Control of Prion Diseases in the Food and Feed Chains

Immunological one‐step determination of the central nervous system indicator proteins, neuron‐specific enolase and glial fibrillary acidic protein, in meat products

Opinion of the Scientific Panel on biological hazards (BIOHAZ) on BSE risk from dissemination of brain particles in blood and carcass following stunning

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