Bioaerosol refers to a state in which biological factors are dispersed as fine particles in a gaseous environment. It has been reported that it not only pollutes indoor air quality but is also included in outdoor atmospheric aerosols, affecting the spread of contagious diseases. Therefore, it is necessary to develop technologies to effectively detect and reduce it. In this study, a nanobubble generating system was developed using the potential energy of nanobubbles and applying an electrochemical process using DSA electrodes for concentrating negative ions on the surface to increase the residence time by improving the stability of nanobubbles. In addition, an air purification system for reducing bioaerosols was developed, and the reduction trend was confirmed through the analysis of ATP activity of Staphylococcus epidermidis, and performance evaluation were performed to optimize operating conditions through airborne microorganism and airborne virus reduction chamber tests. The nanobubble generator was operated under the optimal operating conditions derived from previous research. Experiment were conducted to select the contact method of nanobubbles and bioaerosol in the air purification system, the amount of air flow, and the input amount of feed water. As a result of the experiments, the reduction efficiency trends were changed according to the level of nano-bubble water in the air purification system reaction tank and the amount of air flow. It was confirmed that the method of directly contacting the bioaerosol to the nanobubbles was efficiently reduced compared to the method of spraying the nanobubbles. Finally, the experiment was carried out by spraying and direct contact mixing, and the optimal operating conditions were selected as nanobubble nozzle spraying amount of 10 L/min, blowing amount of 2.25 m 3 /min, and supply water amount of 40 L. When operating the system for 10 minutes, Staphylococcus epidermidis showed a reduction efficiency of about 99% or more, showing the highest reduction tendency. In addition, as a result of the reduction efficiency test of airborne microorganisms and airborne viruses through chamber accreditation tests, it was confirmed that each was reduced by more than 99.9%, and bioaerosols were effectively reduced through the device developed in this study.