Performance evaluation of the Abbott CELL‐DYN Ruby and the Sysmex XT‐2000i haematology analysers

Abstract

Two mid-range haematology analysers (Abbott CELL-DYN Ruby and Sysmex XT-2000i) were evaluated to determine their analytical performance and workflow efficiency in the haematology laboratory. In total 418 samples were processed for determining equivalence of complete blood count (CBC) measurements, and 100 for reticulocyte comparison. Blood smears served for assessing the agreement of the differential counts. Inter-instrument agreement for most parameters was good although small numbers of discrepancies were observed. Systematic biases were found for mean cell volume, reticulocytes, platelets and mean platelet volume. CELL-DYN Ruby WBC differentials were obtained with all samples while the XT-2000i suppressed differentials partially or completely in 13 samples (3.1%). WBC subpopulation counts were otherwise in good agreement with no major outliers. Following first-pass CBC/differential analysis, 88 (21%) of XT-2000i samples required further analyser processing compared to 18 (4.3%) for the CELL-DYN Ruby. Smear referrals for suspected WBC/nucleated red blood cells and platelet abnormalities were indicated for 106 (25.4%) and 95 (22.7%) of the XT-2000i and CELL-DYN Ruby samples respectively. Flagging efficiencies for both analysers were found to be similar. The Sysmex XT-2000i and Abbott CELL-DYN Ruby analysers have broadly comparable analytical performance, but the CELL-DYN Ruby showed superior first-pass efficiency.

Extracorporeal photopheresis as second‐line therapy for patients with acute graft‐versus‐host disease: does the number of cells treated matter?

“…The CELL‐DYN Ruby (Abbott Laboratories, Diagnostics Division) with a 632.8‐nm helium‐neon laser and four optical detectors was used to analyze cell samples. WBC and differential counts were obtained from a single sample dilution, and each of the five main WBC subpopulations (neutrophils, eosinophils, basophils, lymphocytes, and monocytes) was classified on a cell‐by‐cell basis using the four optical scatter signals in a simultaneous process known as multi‐angle polarized scatter separation …”

Verification and quality control of routine hematology analyzers

“… In a verification report, the results for imprecision (repeatability and between batch) should be reported including the mean and CV (%). The state‐of‐the‐art CV (%) for imprecision (reproducibility and between‐batch) is based on the current literature and the experience of the authors. The fourth column represents the ‘Ricos criteria’ that illustrate biological variation , and these performance criteria are based on biological variability.…”

Full Blood Count Estimation Using Abbott Cell-Dyn Ruby 5-Parts in Comparison with Boule Medonic M-Series 3-Parts Differential Haematology Analyser

“…On the other hand, for the parameters whose differences data followed a skewed distribution, the median of the difference at 95% CI interpolation values represented the bias [7]. We established that the two methods can be used interchangeably for a given haematological index if the bias and related 95% CI fell within the limits of clinical acceptability which we had defined a priori in collaboration with clinicians and in consideration of the total allowable errors [20,21].…”