Performance evaluation of the BC-720 auto hematology analyzer and establishment of the reference intervals of erythrocyte sedimentation rate in healthy adults

Shu, Guofang; Ding, Rui; Ding, Rong; Zhi, He; Shen, Yimin; Li, Dongmei; Duan, Zhaohui

doi:10.21037/atm-22-3486

Cited by 2 publications

(2 citation statements)

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“…Therefore, blood samples, even if stored at 4 °C, cannot be analyzed after 2 days. The results of this work on the stability of samples stored at 4 °C agree with what was reported by other authors [ 26 , 27 , 28 , 29 , 30 , 31 , 32 ], who used samples in EDTA, but not with Hu, who used samples diluted in sodium citrate, finding that the samples are stable at 4 °C for up to 12 h. This consideration highlights the greater stability of erythrocyte morphology when using EDTA samples compared to those diluted in sodium citrate, contributing to the standardization of ESR determination [ 18 ]. Moreover, this study investigated for the first time the ESR stability at 4 °C for 36 h and suggests the possibility of test execution up to this time of refrigeration.…”

Section: Discussionsupporting

confidence: 93%

“…The stability evaluation is a crucial aspect of the preanalytical phase to guarantee the diagnostic quality of laboratory results [ 20 ]. Little research has been conducted on the stability of the EDTA whole blood sample at variable times and temperatures for the ESR test [ 27 , 28 ]. The purpose of this work is to assess both the stability of EDTA samples for ESR analysis at room temperature for 24 h (4 h, 6 h, 8 h, 10 h, 24 h) and at 4 °C (24 h, 36 h, and 48 h), and the accuracy with respect to the Westergren method using the VES-MATIC 5 instrument (DIESSE Monteriggioni, Siena).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Stability and Accuracy Compared to the Westergren Method of ESR Samples Analyzed at VES-MATIC 5

Lorubbio,

Diamanti,

Ghiandai

et al. 2024

Diagnostics

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The Erythrocyte Sedimentation Rate (ESR) is a diagnostic estimator of systemic inflammation as a reflection of acute phase proteins circulating in the blood. The purpose of this manuscript is to evaluate the blood stability at room temperature (RT) and at 4 °C to avoid ESR diagnostic errors, as well as the accuracy of the VES-MATIC 5 analyzer. The ESR stability evaluation at RT for 24 h (4 h “T1”, 6 h “T2”, 8 h “T3”, 10 h “T4”, 24 h “T5”) and at 4 °C (24 h, 36 h, 48 h) was carried out using 635 total samples, starting with T0 (2 h of venipuncture). For method comparison, 164 patients were analyzed using VES-MATIC 5 and then the Westergren reference method. The sample at RT is established by a significant gradual decrease in correlation R = 0.99 (T0 vs. T1), R = 0.97 (T0 vs. T2), R = 0.92 (T0 vs. T3), R = 0.87 (T0 vs. T4), and R = 0.40 (T0 vs. T5). The stability at 4 °C after 24 h, 36 h, and 48 h showed a regression of R = 0.99, R = 0.97, and R = 0.95, respectively. Therefore, ESR measurements on RT samples beyond 6 h after collection cannot be carried out, but the ESR can be measured until 36 h for samples stored at 4 °C. Moreover, the VES-MATIC 5 accuracy performance compared to the Westergren method (R = 0.96) is confirmed.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Evaluation of Stability and Accuracy Compared to the Westergren Method of ESR Samples Analyzed at VES-MATIC 5

Lorubbio,

Diamanti,

Ghiandai

et al. 2024

Diagnostics

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Performance evaluation of alternate ESR measurement method using BC-780 automated hematology analyzer: a comparison study with the Westergren reference method

Prompetchara,

Parnsamut,

Wangviwat

et al. 2023

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives Implementation of alternate erythrocyte sedimentation rate (ESR) measurement method is increasing worldwide due to its various advantages. In this study, we aim to evaluate the analytical performance of the BC-780 automated hematology analyzer in measurement of ESR value. Methods Analyzer performance including precision study, carryover, sample stability and potential interferences are examined. Samples with ESR values spanning the whole analytical ESR range are included for method comparison study. Samples with different hematocrit (Hct) and mean corpuscular volume (MCV) values are also analyzed and compared with the results obtained from the Westergren reference method. Results Precisions and carryover results are consistent with the manufacturers’ claim. ESR values do not change significantly in the samples stored at 2–8 °C for 24 h (h) or at room temperature (RT) for 8 h, but significantly decreased (p<0.001) when stored at RT for 24 h. Significant increase in ESR value is documented in samples that are hemolyzed (hemoglobin concentration ranged from 1.28–6.01 g/L) (p=0.010) or lipemic (triglyceride above 4.75 mmol/L) (p=0.001). Method comparison study yields a proportional difference with a regression equation=3.08+ 0.98x. Bland–Altman analysis shows a mean absolute bias of 3.12 mm. The obtained absolute mean biases are below 5 mm in all analytical categories except for the group where MCV>100 fL. Conclusions Most tested parameters met the manufacturer’s specifications and were comparable to the reference method. Despite the presence of positive bias, it falls within acceptable criteria. Extensive validation against potential interferences such as hemolysis/lipemia is still necessary in future.

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Performance evaluation of the BC-720 auto hematology analyzer and establishment of the reference intervals of erythrocyte sedimentation rate in healthy adults

Abstract: Contributions: (I) Conception and design: G Shu, Z Duan; (II) Administrative support: G Shu; (III) Provision of study materials or patients: Rui Ding, Rong Ding, Y Shen; (IV) Collection and assembly of data: Z He, D Liu; (V) Data analysis and interpretation: G Shu;

Cited by 2 publications

References 14 publications

Evaluation of Stability and Accuracy Compared to the Westergren Method of ESR Samples Analyzed at VES-MATIC 5

Evaluation of Stability and Accuracy Compared to the Westergren Method of ESR Samples Analyzed at VES-MATIC 5

Performance evaluation of alternate ESR measurement method using BC-780 automated hematology analyzer: a comparison study with the Westergren reference method

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