Membrane distillation (MD) is a non-isothermal membrane separation process. It is based on the phenomenon that pure water in its vapor state can be extracted from aqueous solutions, with vapor passing through a hydrophobic microporous membrane when a temperature difference is established across it. In this work, three commercially available hydrophobic microporous membranes were used for seawater desalination via direct contact MD. The effects of pertinent operating parameters on the permeation flux have been studied. A plate and frame module was used for seawater desalination. Long-term performance evaluation was carried out to evaluate the process as a stand-alone desalination alternative. The results indicated that polytetrafluoroethylene membrane had the best performance when a hot feed temperature of 80˚C with 800 ml/min flow rate was used. At optimum condition a 99.99% salt rejection was achieved.