Performance levels of four Latin American laboratories for the serodiagnosis of Chagas disease in Mexican sera samples

Luquetti, Alejandro Ostermayer; Espinoza, Bertha; Martı́nez, Ignacio; Hernández-Becerril, Nidia; Ponce, Carlos; Ponce, Elisa; Reyes, Pedro A.; Hernández, Oscar; López, Ruth; Monteón, Vı́ctor

doi:10.1590/s0074-02762009000500023

Cited by 21 publications

(10 citation statements)

References 14 publications

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“…Nonetheless, the high concordance between tests based on the Y and H1 strains, which belong to different lineages, is in agreement with results of studies that showed excellent performance of tests based on a variety of antigen sources with serum from patients in Mexico. 11,12 On the basis of ≥ 2 positive test results, we detected an average seroprevalence of T. cruzi infection of 16.8% (95% CI = 14.2-19.9%) in the study area, which is considerably higher than previous estimates. Seroprevalence rates of 1.3% and 1.8% in the general population and children (< 18 years of age), respectively, have been reported in the jurisdiction of Cordoba, although sample sizes were much smaller and geographic locations were not specified and may have been different.…”

Section: Resultsmentioning

confidence: 51%

Identification of a Hyperendemic Area for Trypanosoma cruzi Infection in Central Veracruz, Mexico

Ramos-Ligonio

López‐Monteon²,

Guzmán-Gómez³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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The state of Veracruz, Mexico, is a well-recognized endemic region for Chagas disease, but the geographic distribution of the disease and its magnitude are still poorly documented. We evaluated the seroprevalence of Trypanosoma cruzi infection in the sanitary jurisdictions of Cordoba and Cosamaloapan in central Veracruz. A total of 654 serum samples from 19 rural localities were tested by using four tests: two enzyme-linked immunosorbent assays, an indirect immunofluorescent, and Western blotting. Overall, 110 (16.8%) of 654 samples were positive for T. cruzi by ≥ 2 tests (95% confidence interval = 14.2–19.9%). The municipality of Tezonapa in the jurisdiction of Cordoba was identified as a potential hyperendemic region with seroprevalence rates ≤ 45% in young children. No cases were detected in the jurisdiction of Cosamaloapan. Further studies should help clarify T. cruzi transmission dynamics in Tezonapa. The magnitude of T. cruzi infection rate in this region calls for the urgent implementation of extensive epidemiologic surveillance and control programs.

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Section: Resultsmentioning

confidence: 51%

Identification of a Hyperendemic Area for Trypanosoma cruzi Infection in Central Veracruz, Mexico

Ramos-Ligonio

López‐Monteon²,

Guzmán-Gómez³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…Also, a recent multicenter study using a panel of serum samples from Mexico confirmed that the geographical origin of the serum samples and the use of T. cruzi I or II as a source of antigens did not affect the performance of serological assays, which included the Stat-Pak rapid test, as well as the Wiener crude extract and recombinant ELISAs (Luquetti et al, 2009). However, as discussed by Otani et al (2009), serum panels are often biased to include “consensus” samples, which may falsely increase the sensitivity of the tests; as such, it remains important to further evaluate the tests in population studies.…”

Section: Discussionmentioning

confidence: 94%

Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?

Gamboa-León

Gonzalez-Ramirez

Padilla-Raygoza

et al. 2011

Journal of Parasitology

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We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8–0.9% of prevalence for T. cruzi–specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.

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“…The antigen for ELISA testing was prepared by means of a mixture of epimastigotes (Luquetti et al 2009) of three strains of T. cruzi (total extract) isolated from triatomines captured in the city of Campeche from a previous study. The mixture was cultivated in LIT media until logarithmic phase was reached, lysed and centrifuged posteriorly.…”

Section: Molecular and Serological Diagnosismentioning

confidence: 99%

Presence of Virginia opossum (Didelphis virginiana) and Pic (Triatoma dimidiata) infected with Trypanosoma cruzi in urban areas: preliminary evaluation in the city of Campeche, Mexico

Tamay-Segovia

Blum-Domínguez

Núñez-Oreza

et al. 2017

Austral j. vet. sci.

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ABSTRACT. The aim of this study was to identify the infection prevalence of Trypanosoma cruzi in opossums and triatomines captured in urban areas of Campeche City, Mexico. We collected 25 specimens of marsupials of Virginia opossums (Didelphis virginiana) species showing a T. cruzi infection prevalence of 52% (13/25). Also, 36 specimens of Pic (Triatoma dimiata) vectors were collected, showing a T. cruzi infection prevalence of 41.6% (15/36). All T. cruzi isolates were TcI, which correspond to the predominant lineage in mammals of Mexico, irrespective of the mammalian host species or vector.Key words: Didelphis virginiana, Triatoma dimidiata, Trypanosoma cruzi, Chagas Disease. RESUMEN.El objetivo del estudio fue identificar la prevalencia de infección con Trypanosoma cruzi en zarigüeyas (Didelphis virginiana) y Pic (Triatoma dimidiata) capturados en áreas urbanas de la ciudad de Campeche, México. Se colectaron 25 zarigüeyas con una prevalencia de infección con T. cruzi del 52% (13/25). También se colectaron 36 vectores de Pic con una prevalencia de infección del 41,6% (15/36) con T. cruzi. Todos los aislados de T. cruzi fueron TcI de acuerdo con la predominancia de este linaje en México independientemente del huésped mamífero o vector.

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Performance levels of four Latin American laboratories for the serodiagnosis of Chagas disease in Mexican sera samples

Abstract: In nearly all of the previous multicentre studies evaluating serological

Cited by 21 publications

References 14 publications

Identification of a Hyperendemic Area for Trypanosoma cruzi Infection in Central Veracruz, Mexico

Identification of a Hyperendemic Area for Trypanosoma cruzi Infection in Central Veracruz, Mexico

Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?

Presence of Virginia opossum (Didelphis virginiana) and Pic (Triatoma dimidiata) infected with Trypanosoma cruzi in urban areas: preliminary evaluation in the city of Campeche, Mexico

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