Performance Modelling of the Bioelectrochemical Glycerol Oxidation by a Co‐Culture of <i>Geobacter Sulfurreducens</i> and <i>Raoultella Electrica</i>

Kubannek, Fabian; Thiel, Simone; Bunk, Boyke; Huber, Katharina J.; Overmann, Jörg; Krewer, Ulrike; Biedendieck, Rebekka; Jahn, Dieter

doi:10.1002/celc.202000027

Cited by 8 publications

(7 citation statements)

References 49 publications

(74 reference statements)

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“…These results implied that the various co-cultures and ternary culture systems could utilize CMC to generate electricity and that the maximum current density (796 ± 30 μA cm −2 ) of the ternary culture using CMC was much higher than those other cultures. The ternary consortium also achieved the highest CMCbioelectricity generation comparing with previously reported results for the co-culture system (Table S3) (32)(33)(34)(35). Evidence for Electron shuttles Involved in E-BG.…”

Section: Resultssupporting

confidence: 84%

The enhancement of electricity generation using cellulose based on ternary microbial consortium

Gao,

Mai,

Ding

et al. 2024

Preprint

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Cooperation between microorganisms is crucial to design an efficient inoculum for enhancing the electricity-producing ability of carboxymethyl cellulose (CMC) fed bioreactors. In the present study, the influence of microbial mutualistic interactions and electricity generation capability were investigated by designing several co-culture and ternary culture systems. It was found that a ternary culture system of Cellulomonas Lsc-8, Bacillus subtilis C9 and Geobacter sulfurreducens PCA was used to efficiently convert cellulose into electricity. The maximum current density of 796 ± 30 μA·cm-2were achieved by the ternary culture, which were much higher than that Geobacter sulfurreducens PCA using acetate and co-culture systems to utilize CMC in bioreactors, respectively. In this consortium, Cellulomonas Lsc-8, and Bacillus subtilis C9 simultaneously digested CMC to produce acetate and secreted riboflavin as an electron shuttle; Geobacter sulfurreducens PCA utilized acetate to generate electricity. The introduction of Bacillus subtilis C9 further promoted the degradation of CMC and secreted more riboflavin to enhance electricity generation of the ternary culture. This work suggested that the synergistic interaction between interspecies in microbial consortia is emergent in designing specific community for achieving maximum power generation using CMC as substrate. This research shows new insight into the design of more efficient, stable, and robust microbial consortia applicable in waste treatment and power generation.

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Section: Resultssupporting

confidence: 84%

The enhancement of electricity generation using cellulose based on ternary microbial consortium

Gao,

Mai,

Ding

et al. 2024

Preprint

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“…Model-based kinetic analysis of the surface processes shows that by employing steady-state OER experiments, microkinetic parameters could not be identified. , In contrast, combining dynamic experiments, such as cyclic voltammetry (CV), impedance spectroscopy, or chronopotentiometry/amperometry, with dynamic microkinetic modeling allows to elucidate the surface processes and effects of further chemical or transport processes and to study limitations. This has been demonstrated by revealing the reaction kinetics of acidic , and alkaline methanol oxidation including catalyst passivation by the kinetics and poisoning of the cathode in PEM fuel cells and even for kinetics that involve chemical reactions in the electrolyte or bioelectrochemical reactions . Recently, it was applied to the OER on planar hydrous Ir to identify the kinetic rate constants and the OER mechanism by using CV curves .…”

Section: Introductionmentioning

confidence: 99%

“…This has been demonstrated by revealing the reaction kinetics of acidic 22,23 and alkaline methanol oxidation including catalyst passivation 24 by the kinetics 25 and poisoning 26 of the cathode in PEM fuel cells and even for kinetics that involve chemical reactions in the electrolyte 27 or bioelectrochemical reactions. 28 Recently, it was applied to the OER on planar hydrous Ir to identify the kinetic rate constants and the OER mechanism by using CV curves. 29 No work on kinetic identification based on experimental CV curves of nanoparticulate OER catalysts and on rutile IrO 2 as the technical state-of-the-art OER catalyst is available; however, such analysis would give in-depth insight into the performance limitations and may trigger improved commercial catalysts and electrolysis.…”

Section: Introductionmentioning

confidence: 99%

Microkinetic Analysis of the Oxygen Evolution Performance at Different Stages of Iridium Oxide Degradation

et al. 2022

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The microkinetics of the electrocatalytic oxygen evolution reaction substantially determines the performance in proton-exchange membrane water electrolysis. State-of-the-art nanoparticulated rutile IrO 2 electrocatalysts present an excellent trade-off between activity and stability due to the efficient formation of intermediate surface species. To reveal and analyze the interaction of individual surface processes, a detailed dynamic microkinetic model approach is established and validated using cyclic voltammetry. We show that the interaction of three different processes, which are the adsorption of water, one potential-driven deprotonation step, and the detachment of oxygen, limits the overall reaction turnover. During the reaction, the active IrO 2 surface is covered mainly by *O, *OOH, and *OO adsorbed species with a share dependent on the applied potential and of 44, 28, and 20% at an overpotential of 350 mV, respectively. In contrast to state-of-the-art calculations of ideal catalyst surfaces, this novel model-based methodology allows for experimental identification of the microkinetics as well as thermodynamic energy values of real pristine and degraded nanoparticles. We show that the loss in electrocatalytic activity during degradation is correlated to an increase in the activation energy of deprotonation processes, whereas reaction energies were marginally affected. As the effect of electrolyte-related parameters does not cause such a decrease, the model-based analysis demonstrates that material changes trigger the performance loss. These insights into the degradation of IrO 2 and its effect on the surface processes provide the basis for a deeper understanding of degrading active sites for the optimization of the oxygen evolution performance.

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“…A key aspect of performance optimization in BES lies in the knowledge and comprehensive understanding of the underlying reaction kinetics. Furthermore, the kinetic expression are an essential part of most BES models [ 1 , 2 , 3 , 4 , 5 ]. Unfortunately, the reaction kinetics are not well established in the bioelectrochemical literature.…”

Section: Introductionmentioning

confidence: 99%

“…One of the most important features of the chemostat is that it allows the operator to control μ by adjusting the averaged hydraulic retention time. For a BES, the same approaches apply, except that in the biomass balance the free, planktonic biomass concentration is extended by the term of the immobilized biomass concentration, which is retained by cell immobilization on an electrode resulting in the accumulation of biomass [ 2 , 3 ]. If the BES is operated continuously, this corresponds to a retentostat process.…”

Section: Introductionmentioning

confidence: 99%

Reaction kinetics of anodic biofilms under changing substrate concentrations: Uncovering shifts in Nernst‐Monod curves via substrate pulses

Kubannek

Block

Munirathinam

et al. 2022

Engineering in Life Sciences

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In the present study, it is shown that the concentration dependency of undefined mixed culture anodic biofilms does not follow a single kinetic curve, such as the Nernst-Monod curve. The biofilms adapt to concentration changes, which inevitably have to be applied to record kinetic curves, resulting in strong shifts of the kinetic parameters. The substrate concentration in a continuously operated bioelectrochemical system was changed rapidly via acetate pulses to record Nernst-Monod curves which are not influenced by biofilm adaptation processes. The values of the maximum current density j max and apparent half-saturation rate constant K s increased from 0.5 to 1 mA cm −2 and from 0.5 to 1.6 mmol L −1 , respectively, within approximately 5 h. Double pulse experiments with a starvation phase between the two acetate pulses showed that j max and K s decrease reversibly through an adaptation process when no acetate is available. Pseudocapacitive charge values estimated from non-turnover cyclic voltammograms (CV) led to the hypothesis that biofilm adaptation and the observed shift of the Nernst-Monod curves occurred due to changes in the concentration of active redox proteins in the biofilm. It is argued that concentration-related parameters of kinetic models for electroactive biofilms are only valid for the operating points where they have been determined and should always be reported with those conditions.

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Performance Modelling of the Bioelectrochemical Glycerol Oxidation by a Co‐Culture of Geobacter Sulfurreducens and Raoultella Electrica

Cited by 8 publications

References 49 publications

The enhancement of electricity generation using cellulose based on ternary microbial consortium

The enhancement of electricity generation using cellulose based on ternary microbial consortium

Microkinetic Analysis of the Oxygen Evolution Performance at Different Stages of Iridium Oxide Degradation

Reaction kinetics of anodic biofilms under changing substrate concentrations: Uncovering shifts in Nernst‐Monod curves via substrate pulses

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