Performance of 18S rDNA helix E23 for phylogenetic relationships within and between the Rotifera–Acanthocephala clades

Miquelis, Anne; Martin, Jean‐François; Carson, Evan W.; Brun, Guy Le; Gilles, André

doi:10.1016/s0764-4469(00)01230-0

Cited by 17 publications

(16 citation statements)

References 45 publications

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“…Two mitochondrial (mt) and two nuclear (nu) DNA fragments were ampliWed by standard PCR techniques. Primers used for ampliWcation were those described by Salducci et al (2005) for 16S and 12S, by Bossuyt and Milinkovitch (2000) for tyrosinase, and by Miquelis et al (2000) for 18S rDNA. The chosen molecular markers previously have been used successfully to assess the relationships among orders, families and species of amphibians (Hay et al, 1995;Ruvinsky and Maxson, 1996;Vences et al, 2000).…”

Section: Dna Protocolsmentioning

confidence: 99%

Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups

Fouquet

Vences

Salducci³

et al. 2007

Molecular Phylogenetics and Evolution

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Few studies to date have examined genetic variability of widespread tropical amphibian species over their distributional range using diVerent kinds of molecular markers. Here, we use genetic data in an attempt to delimit evolutionary entities within two groups of Neotropical frogs, the Scinax ruber species group and the Rhinella margaritifera species group. We combined mitochondrial and nuclear markers for a phylogenetic (a total of »2500 bp) and phylogeographic study (»1300 bp) to test the reliability of the currently accepted taxonomic assignments and to explore the geographic structure of their genetic variation, mainly based upon samples from the French Guianan region. Phylogenetic analyses demonstrated the polyphyly of Scinax ruber and Rhinella margaritifera. S. ruber consists of six lineages that may all merit species status. ConXicting signals of mitochondrial and nuclear markers indicated, among some Scinax lineages and species, the possibility of ongoing hybridization processes. R. margaritifera consisted of 11 lineages which might represent distinct species as well. Phylogeographic analyses added further information in support of the speciWc status of these lineages. Lineages of low divergence were found in sympatry and were reciprocally monophyletic for mitochondrial as well as nuclear genes, indicating the existence of young lineages that should be awarded species status. Our results highlight the utility of combining phylogenetic and phylogeographic methods, as well as the use of both mitochondrial and nuclear markers within one study. This approach helped to better understand the evolutionary history of taxonomically complex groups of species. The assessment of the geographic distribution of genetic diversity in tropical amphibian communities can lead to conclusions that diVer strongly from prior analyses based on the occurrence of currently recognized species alone. Such studies, therefore, hold the potential to contribute to a more objective assessment of amphibian conservation priorities in tropical areas.

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Section: Dna Protocolsmentioning

confidence: 99%

Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups

Fouquet

Vences

Salducci³

et al. 2007

Molecular Phylogenetics and Evolution

Self Cite

134

143

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“…However, these studies have contributed further to the confusion and proposed acanthocephalans as the sister group to either Seisonidea (Zrzavý, 2001a;Herlyn et al, 2003), Bdelloidea (Garey et al, 1996 Miquelis et al, 2000). In spite of these incongruities, it is, however, noteworthy that all studies that involve molecular evidences unambiguously support Acanthocephala as a rotifer ingroup (but see Near, 2002).…”

Section: Acanthocephalans and Their Implications For Rotifer Monophylymentioning

confidence: 99%

“…(Lorenzen, 1985;Ahlrichs, 1995aAhlrichs, , 1997Ahlrichs, , 1998Ferraguti and Melone, 1999;Sørensen et al, 2000), but all these have been based on dubious homology statements or contradictory details in sperm anatomy (Melone et al, 1998a;Ricci, 1998b;Funch et al, 2005). Hence, morphological data cannot at present provide critical support for Acanthocephala as a rotifer ingroup, although a steadily growing amount of molecular data strongly support its inclusion (Garey et al, 1996Near et al, 1998;Giribet et al, 2000Giribet et al, , 2004Miquelis et al, 2000;Near, 2002).…”

Section: Introductionmentioning

confidence: 99%

A modern approach to rotiferan phylogeny: Combining morphological and molecular data

Sørensen

Giribet

2006

Molecular Phylogenetics and Evolution

142

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“…This also was supported by phylogenetic analyses using 18S rDNA (Miquelis et al, 2000). A cladistic study using morphological characters gave some additional support for this relationship (Sørensen et al, 2000), since spermatozoa of both monogononts and acanthocephalans lack an acrosome.…”

Section: Evaluation Of the Phylogenetic Position Of Acanthocephalamentioning

confidence: 67%

On the Phylogenetic Position of Rotifera – Have We Come Any Further?

2005

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Rotifers are bilateral symmetric animals belonging to Protostomia. The ultrastructure of the rotiferan trophi suggests that they belong to the Gnathifera, and ultrastructural similarities between the integuments and spermatozoa as well as molecular evidence strongly suggest that rotifers and the parasitic acanthocephalans are closely related and form the clade Syndermata. Here we discuss the phylogenetic position of rotifers with regard to the gnathiferan groups.

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Performance of 18S rDNA helix E23 for phylogenetic relationships within and between the Rotifera–Acanthocephala clades

Cited by 17 publications

References 45 publications

Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups

Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups

A modern approach to rotiferan phylogeny: Combining morphological and molecular data

On the Phylogenetic Position of Rotifera – Have We Come Any Further?

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