Performance of 3 real-time PCR assays for direct detection of Staphylococcus aureus and MRSA from clinical samples

Mehta, Maitry S.; McClure, J. Trenton; Mangold, Kathy A.; Peterson, Lance R.

doi:10.1016/j.diagmicrobio.2014.06.005

Cited by 11 publications

(3 citation statements)

References 21 publications

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“…The conventional detection method of S. aureus was based on microbial culturing, followed by biochemical identification and antimicrobial susceptibility testing. Molecular biological assays for S. aureus like polymerase chain reaction (PCR) ( 27 ), real-time PCR ( 28 ) and Multiplex real-time PCR ( 29 ) are commonly used. In addition, high-performance liquid chromatography (HPLC) ( 30 ) and enzyme-linked immunosorbent assay (ELISA) ( 31 ) are also used.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification

Cheng

Zhen

et al. 2023

Microbiol Spectr

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Our study established IMBs-RPA method, which simplified the steps of bacteria separation, shortened the detection time, and realized the convenient detection of S. aureus in milk and pork samples. IMBs-RPA method was also suitable for the detection of other pathogens, providing a new method for food safety monitoring and a favorable basis for rapid and early diagnosis of diseases.

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Section: Introductionmentioning

confidence: 99%

Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification

Cheng

Zhen

et al. 2023

Microbiol Spectr

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“…The traditional bacterial culture method for the detection of S. aureus, although inexpensive and commonly used in resource-limited settings, the method is time-consuming and not able to meet the requirements of rapid detection [6]. PCR method, although highly sensitive, but the instrument is expensive and sophisticated, requires highly qualified personnel to operate, and is difficult to popularize [7]. The traditional ELISA method for the detection of S. aureus is characterized by low chemical stability, high cost, and difficulty in recycling [8].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for Detection of <i>Staphylococcus aureus</i> Based on Nucleic Acid Aptamers

Liang,

2023

JBM

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Staphylococcus aureus is a gram-staining positive cocci bacillus baterium and also one of the foodborne pathogens, which is a serious potential hazard to human health and food safety. We constructed an electrochemical biosensor for the detection of S. aureus based on nucleic acid aptamers to achieve highly specific detection of S. aureus. The detection of S. aureus was realized by using Aptamer (Apt) to capture S. aureus, which resulted in a change in the spatial conformation of Apt and a decrease in the electrochemical signal. Under the optimized experimental conditions, the detected electrochemical signals were positively correlated with the concentration of S. aureus with a linear range of 1 × 10 1 -1 × 10 5 CFU/mL, a detection limit of 4.76 CFU/mL, and an experimental recovery of 97.43% -99.37%. Therefore, we successfully constructed an electrochemical biosensor for the specific detection of S. aureus, which has the advantages of high specificity, sensitive detection and convenient operation.

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“…Polymerase chain reaction (PCR) tests and ELISA (enzyme-linked immunosorbent assay) are also used in S. aureus detection; beyond the present low limit of detection (LoD), they are more expensive and require more qualified professionals to operate. , Not even the PCR, ELISA, or bacterial culture matches the prerequisites for a rapid test . The detection of S.…”

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confidence: 99%

Point-of-Care Electrochemical Immunosensor Applied against Nosocomial Infection: Staphylococcus aureus Detection in Human Hand Skin

de Godoy,

Faria,

Roza

et al. 2024

ACS Infect. Dis.

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Staphylococcus aureus is an important pathogen that causes nosocomial infections, resulting in unacceptable morbidity and mortality rates. In this work, we proposed the construction of a nanostructured ZnO-based electrochemical immunosensor for qualitative and semiquantitative detection of S. aureus using simple methods for growing zinc oxide nanorods (ZnO NRs) on a sensor board and immobilizing the anti-S. aureus antibody on ZnO NRs through cystamine and glutaraldehyde. The immunosensor detected S. aureus in the 103–107 colony-forming unit (CFU) mL–1 range and showed a limit of detection (LoD) around 0.792 × 103 CFU mL–1. Beyond a satisfactory LoD, the developed immunosensor presented other advantages, such as high versatility for point-of-care assays and a suitable selective factor that admits the detection of the S. aureus concentration range in human hand skin after washing. Moreover, the immunosensor showed the potential to be an excellent device to control nosocomial infection by detecting the presence of S. aureus in human hand skin.

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Performance of 3 real-time PCR assays for direct detection of Staphylococcus aureus and MRSA from clinical samples

Cited by 11 publications

References 21 publications

Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification

Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification

Electrochemical Biosensor for Detection of <i>Staphylococcus aureus</i> Based on Nucleic Acid Aptamers

Point-of-Care Electrochemical Immunosensor Applied against Nosocomial Infection: Staphylococcus aureus Detection in Human Hand Skin

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Performance of 3 real-time PCR assays for direct detection of Staphylococcus aureus and MRSA from clinical samples

Cited by 11 publications

References 21 publications

Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification

Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification

Electrochemical Biosensor for Detection of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; Based on Nucleic Acid Aptamers

Point-of-Care Electrochemical Immunosensor Applied against Nosocomial Infection: Staphylococcus aureus Detection in Human Hand Skin

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Electrochemical Biosensor for Detection of <i>Staphylococcus aureus</i> Based on Nucleic Acid Aptamers