Performance of a full-scale modified anaerobic/anoxic/oxic process: High-throughput sequence analysis of its microbial structures and their community functions

Chen, Yasong; Zhao, Zheng; Peng, Yuke; Li, Jie; Xiao, Lin; Yang, Liuyan

doi:10.1016/j.biortech.2016.07.095

Cited by 107 publications

(40 citation statements)

References 35 publications

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“…Although the members of Anaerolineae have been described as strictly anaerobic 35 , 36 , Chen et al . 22 also found a high representation of Anaerolineae in the aerobic treatment unit of a WWTP which is located only 150 kilometers away from the QG-A2O WWTP of this study. Both Chen’s WWTP and QG-A2O adopt A2/O processes and treat domestic sewage.…”

Section: Discussionsupporting

confidence: 64%

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Seasonal bacterial community succession in four typical wastewater treatment plants: correlations between core microbes and process performance

Zhang

Yu²,

Yan

et al. 2018

Sci Rep

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To understand the seasonal variation of the activated sludge (AS) bacterial community and identify core microbes in different wastewater processing systems, seasonal AS samples were taken from every biological treatment unit within 4 full-scale wastewater treatment plants. These plants adopted A2/O, A/O and oxidation ditch processes and were active in the treatment of different types and sources of wastewater, some domestic and others industrial. The bacterial community composition was analyzed using high-throughput sequencing technology. The correlations among microbial community structure, dominant microbes and process performance were investigated. Seasonal variation had a stronger impact on the AS bacterial community than any variation within different wastewater treatment system. Facing seasonal variation, the bacterial community within the oxidation ditch process remained more stable those in either the A2/O or A/O processes. The core genera in domestic wastewater treatment systems were Nitrospira, Caldilineaceae, Pseudomonas and Lactococcus. The core genera in the textile dyeing and fine chemical industrial wastewater treatment systems were Nitrospira, Thauera and Thiobacillus.

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Section: Discussionsupporting

confidence: 64%

“…All plants operated stably whilst performing carbon and nitrogen removal. QG-A2O was also subjected to an enhanced biological phosphorus removal process 20 , 22 , 29 . In winter, their auxiliary heating system would maintain the biological treatment units at temperatures not lower than 13 °C 30 .…”

Section: Resultsmentioning

confidence: 99%

Seasonal bacterial community succession in four typical wastewater treatment plants: correlations between core microbes and process performance

Zhang

Yu²,

Yan

et al. 2018

Sci Rep

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“…The reaction mixtures and cycling conditions were the same as for the first round, with modifications of the template using the product of the first amplification. After purification, nested PCR products were quantified using QuantiFluor™-ST (Promega, USA) (Chen et al 2016 ), and then normalized in equimolar ratios and sequenced using the Illumina MiSeq platform. Raw sequencing reads were deposited in the NCBI Sequence Read Archive with accession number SRX3209560.…”

Section: Methodsmentioning

confidence: 99%

Chronic fertilization of 37 years alters the phylogenetic structure of soil arbuscular mycorrhizal fungi in Chinese Mollisols

Ongena

Wang

et al. 2018

AMB Expr

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Arbuscular mycorrhizal fungi (AMF) play vital roles in sustaining soil productivity and plant communities. However, adaption and differentiation of AMF in response to commonly used fertilization remain poorly understood. In this study, we showed that the AMF community composition was primarily driven by soil physiochemical changes associated with chronic inorganic and organic fertilization of 37 years in Mollisols. High-throughput sequencing indicated that inorganic fertilizer negatively affected AMF diversity and richness, implying a reduction of mutualism in plant–AMF symbiosis; however, a reverse trend was observed for the application of inorganic fertilizer combined with manure. With regards to AMF community composition, order Glomerales was dominant, but varied significantly among different fertilization treatments. All fertilization treatments decreased family Glomeraceae and genus Funneliformis, while Rhizophagus abundance increased. Plant-growth-promoting-microorganisms of family Claroideoglomeraceae and genus Claroideoglomus were stimulated by manure application, and likely benefited pathogen suppression and phosphorus (P) acquisition. Family Gigasporaceae and genus Gigaspora were negatively correlated with available P in soil. Additionally, redundancy analysis further suggested that soil available P, organic matter and pH were the most important factors in shaping AMF community composition. These results provide strong evidence for niche differentiation of phylogenetically distinct AMF populations under different fertilization regimes. Manure likely contributes to restoration and maintenance of plant–AMF symbiosis, and the balanced fertilization would favor the growth of beneficial AMF communities as one optimized management in support of sustainable agriculture in Mollisols.

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“…Glucose was used as cosubstrate and the concentration of influent 2‐chlorophenol was increased gradually. Changes in microbial community composition and the diversity of microbes in the activated sludge were investigated using high‐throughput sequencing (HTS), which is widely used in research into biological wastewater treatment (Chen et al., ; Wang, Miao, Kong, & Ni, ; Ye & Zhang, ). The results of this study can potentially be applied in the biodegradation of 2‐chlorophenol and provide useful guidance for the biodegradation of chlorophenols.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Acclimation of 2‐chlorophenol‐biodegrading activated sludge and microbial community analysis

Sun

Zhang

Yang

et al. 2019

Water Environment Research

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Using glucose as cosubstrate, activated sludge that could effectively biodegrade 40 mg/L 2‐chlorophenol was successfully domesticated in sequencing batch reactors. To acclimate the sludge, 2‐chlorophenol was increased stepwise from 0 to 40 mg/L. High‐throughput sequencing revealed that the microbial community richness increased during the first 5 days of acclimation to 5 mg/L 2‐chlorophenol and then decreased after another 20 days as 2‐chlorophenol was increased. The original sludge obtained from a water resource recovery facility had the highest microbial diversity. As the acclimation continued further, community richness and diversity both increased, but they decreased again, significantly, when 2‐chlorophenol reached 40 mg/L. Saccharibacteria_norank, Bacillus, Saprospiraceae_uncultured, and Lactococcus were the dominant bacteria. Bacillus and Pseudomonas were the main known chlorophenol‐degrading bacteria. WCHB1‐60_norank, Tetrasphaera, Comamonadaceae_unclassified, and Haliangium showed poor tolerance to 2‐chlorophenol. Higher bacterial tolerance to chlorophenols does not mean higher degrading capability. The degradation of chlorophenols was not positively correlated with the detected abundance of known 2‐chlorophenol‐degrading bacteria. Practitioner points Activated sludge that could effectively biodegrade 40 mg/L 2‐chlorophenol was successfully domesticated using glucose as cosubstrate in sequencing batch reactors. Saccharibacteria_norank, Bacillus, Saprospiraceae_uncultured, and Lactococcus were the dominant bacteria. Bacillus and Pseudomonas were the main known chlorophenol‐degrading bacteria detected in this study. The degrading capability and tolerance of bacteria to chlorophenols were relatively independent and the degradation of chlorophenols may be the synergistic effect of various bacteria.

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Performance of a full-scale modified anaerobic/anoxic/oxic process: High-throughput sequence analysis of its microbial structures and their community functions

Cited by 107 publications

References 35 publications

Seasonal bacterial community succession in four typical wastewater treatment plants: correlations between core microbes and process performance

Seasonal bacterial community succession in four typical wastewater treatment plants: correlations between core microbes and process performance

Chronic fertilization of 37 years alters the phylogenetic structure of soil arbuscular mycorrhizal fungi in Chinese Mollisols

Retracted: Acclimation of 2‐chlorophenol‐biodegrading activated sludge and microbial community analysis

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