Performance of a modified ovum pick-up system using three different FSH stimulation protocols in ewes

Alberio, R. H.; Olivera, Julio; Roche, A.; Alabart, J. L.; Folch, J.

doi:10.1016/s0921-4488(02)00157-8

Cited by 31 publications

(35 citation statements)

References 9 publications

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“…Teixeira et al (6) , utilizando a mesma técnica de aspiração folicular, obtiveram resultado inferior em número de oócitos (5,8 ± 2,3) e taxa de recuperação semelhante (51,7%) e Alberio et al (17) relataram resultados inferiores tanto em número de oócitos (4,9 ± 1,3) quanto na taxa de recuperação (33,6%). De forma semelhante, os resultados desse experimento divergem dos relatados por Wieczorek et al (3) (3,1 oócitos/doadora), Rodriguez et al (13) (8,7 oócitos/doadora), Gibbons et al (10) (6,8 oócitos/doadora) e Avelar et al (15) (11,1 oócitos/doadora), que realizaram aspirações foliculares por laparoscopia.…”

Section: Resultsunclassified

“…Esse resultado pode ter sido influenciado pelos diversos fatores relacionados à técnica e aos equipamentos utilizados, dentre os quais, de grande relevância, o diâmetro da agulha e o fluxo e a pressão de aspiração, que influenciam diretamente a qualidade oocitária (13) . A viabilidade oocitária, objetivo final da aspiração folicular como passo inicial para o desenvolvimento de biotecnologias como a produção in vitro de embriões, foi satisfatória, alcançando-se resultados superiores e semelhante aos resultados obtidos por Rodriguez et al (13) (58,1%) e Gibbons et al (10) (63,2%); entretanto, inferior aos resultados de Max et al (18) (81,6%), Alberio et al (17) (87,0%) e Koeman et al (12) , em dois grupos estudados, fêmeas adultas (82,0%) e pré-púberes (87,0%). Oócitos considerados viáveis são aqueles classificados como Grau I, II e III; por isso, uma vez que a qualidade oocitária for baixa, refletirá diretamente na viabilidade.…”

Section: Resultsunclassified

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Aspiração Folicular Videolaparoscópica Comparativa Em Ovelhas Dorper E Santa Inês

Araújo

Oliveira

Tabet³

et al. 2016

Ciênc. anim. bras.

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ResumoEste estudo teve por objetivo comparar o número de folículos aspirados, oócitos recuperados, qualidade e viabilidade oocitária entre ovelhas das raças Dorper e Santa Inês, submetidas à aspiração folicular por vídeolaparoscopia. Para realização deste estudo, foram efetuadas 118 aspirações foliculares, das quais 65 foram realizadas em ovelhas da raça Dorper e 53 em ovelhas da raça Santa Inês. Os resultados encontrados para as ovelhas Dorper foram 28,31 ± 17,42 folículos/ovelha, 12,21 ± 6,74 oócitos/ovelha, taxa de recuperação de 47,9% e 8,93 ± 5,25 oócitos viáveis/ovelha. Para as ovelhas Santa Inês foram, 22,14 ± 16,08 folículos/ovelha, 12,64 ± 8,29 oócitos/ovelha, taxa de recuperação de 55% e 8,92 ± 6,58 oócitos viáveis/ovelha. Não houve influência das raças sobre o número de folículos aspirados, oócitos recuperados, taxa de recuperação, qualidade e viabilidade oocitária em aspiração folicular por vídeolaparoscopia. Palavras-chave: folículo; oócito; ovino. AbstractThis study aimed to compare the number of aspirated follicles, recovered oocytes, oocyte quality and viability between Dorper and Santa Inês ewes submitted to the videolaparoscopic ovum pick-up. To conduct this study, 118 follicular aspirations were performed, of which 65 were performed in Dorper ewes and 53 in Santa Ines ewes. The results for Dorper sheep were 28.31 ± 17.42 follicles/ewe, 12.21 ± 6.74 oocytes/ewe, recovery rate of 47.9% and 8.93 ± 5.25 viable oocytes/ewe. For Santa Inês ewes the results were 22.14 ± 16.08 follicles/ewe, 12.64 ± 8.29 oocytes/ewe, recovery rate of 55% and 8.92 ± 6.58 viable oocytes/ewe. There was no influence of breed on the number of aspirated follicles, recovered oocytes, recovery rate, oocyte quality and viability in videolaparoscopic ovum pick-up.

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Section: Resultsunclassified

Aspiração Folicular Videolaparoscópica Comparativa Em Ovelhas Dorper E Santa Inês

Araújo

Oliveira

Tabet³

et al. 2016

Ciênc. anim. bras.

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“…Several authors reported oocyte recovery rates ranging from 16 to 98% (Alberio et al, 2002;Gibbons et al, 2007;Teixeira et al, 2011;Cordeiro et al, 2014) in small ruminants. Due to the shortage of LapOPU data in rodents, it can be assumed that the recovery rate achieved in our study (32.56±27.32%) is in accordance with what Rodríguez et al (2006) considered normal (from 32 to 90%).…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic ovum pick-up in spotted paca ( Cuniculus pacas )

Barros

Teixeira

Uscátegui

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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The aim of this work is study the laparoscopic ovum pick-up (LapOPU) technique in spotted paca, describing surgery details, complications and oocyte recovery rate. Nine healthy adult non-pregnant captive females were used, in a total of 39 procedures. When the surgical plane of anaesthesia was achieved, the females were positioned at 20º Trendelenburg. Three 6mm trocars were placed on right and left inguinal and hypogastric regions. Abdomen was inflated with CO 2 and the intra-abdominal pressure was stablished in 10mmHg. Follicular punctures were performed moving the ovaries with atraumatic forceps. For punctures, an 18-gauge 3.5 inch long needle attached to a vacuum system with pressure not exceeding 65mmHg was used. Oocytes were recovered into 50mL centrifuge tubes with media composed of PBS supplemented with 10 IU/mL of heparin and kept at 36°C. R Software was used for statistical analysis. Data normality distribution (Shapiro test) and variances homoscedasticity (Bartlett test) were tested and descriptive statistics (mean±SD) was used to present the results. It was only possible to perform LapOPU in 30 of 39 laparoscopies (76.92%). The surgical total time was 37.34 ± 18.53 minutes. The total number of visualized follicles, aspirated follicles, and retrieved oocytes were 502, 415, and 155, respectively. And the same parameters per animal were: 14.34 ± 12.23, 11.86 ± 10.03, and 4.43 ± 4.69 respectively. Oocyte recovery rate was 32.56 ± 27.32%. In conclusion, caudal positioning of portals with slight triangulation allows good viewing of the abdominal cavity and eases the manipulation of the ovaries. Thus this described LapOPU technique is feasible in spotted paca and easy to perform. 34 ± 12,23, 11,86 ± 10,03 e 4,43 ± 4,69, respectivamente Keywords

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“…This method is often called as laparoscopic ovum pick-up (LOPU) and seems to be very efficient for the recovery and quality of the COCs obtained in sheep and goats. Usually, in sheep, a short repeated gonadotrophin treatment is followed by LOPU [35][36][37][38]. In contrast, goats can be stimulated with a 'one shot' regime of gonadotrophins (one shot of follicular stimulating hormone (FSH) plus equine chorionic gonadotrophin (eCG), compared to the traditional multi-dose and F), insertion of trocar and cannula, cannula is left on the abdomen through which endoscope is inserted into the abdomen; (G), insertion of the endoscope through the cannula to visualize the ovaries, corpora lutea and reproductive tract; (H), a uterine horn is punctured close to the utero-tubular junction using a blunt-end 18 Gauge needle, embryos are loaded into an ET catheter and transferred into the lumen.…”

Section: Oocyte Collection and In Vitro Maturationmentioning

confidence: 99%