Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF

Han, Fengxuan; Jia, Xiaoling; Dai, Dongdong; Yang, Xiaoling; Zhao, Jin; Zhao, Yunhui; Fan, Yubo; Yuan, Xiaoyan

doi:10.1016/j.biomaterials.2013.06.006

Cited by 156 publications

(143 citation statements)

References 46 publications

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“…[9][10][11] To date, a variety of natural and synthetic electrospun polymers have been used extensively to prepare vascular scaffolds. [12][13][14] Natural polymers have often been used to prepare vascular scaffolds based on their biocompatibility profiles. For example, collagen and elastin (the primary structural components of the extracellular matrix in vascular tissues) have been electrospun into fibrous scaffolds for vascular grafting applications.…”

Section: Introductionmentioning

confidence: 99%

“…12,17 On the other hand, synthetic electrospun scaffolds containing materials such as polycaprolactone (PCL), poly(L-lactide-co-ε-caprolactone) (PLCL), and poly(L-lactide-co-glycolide) have been shown to have good mechanical strength and tunable biodegradability. 13,18,19 Interestingly, more promising results have been achieved by combining natural and synthetic polymers. Previous reports indicate that an electrospun scaffold containing both PCL and collagen had excellent tensile strength (4.0±0.4 MPa) and burst pressure (4,912±155 mmHg), comparable with native vessels.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Fu¹,

Liu²,

Feng³

et al. 2014

IJN

212

117

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Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-ε-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young’s modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Fu¹,

Liu²,

Feng³

et al. 2014

IJN

212

117

View full text Add to dashboard Cite

show abstract

“…42 Future research may elaborate on the results in this study and may include the (in vitro) preparation and testing of a range of grafts with different pore sizes, thinner walls and/or incorporation of a synthetic polymer mesh or support film. 24,25 Improvements to reduce the wall thickness may include performing controlled compression of the graft's porous wall during construction.…”

Section: Discussionmentioning

confidence: 99%

“…However, in multiple reported cases, the electrospun materials' small pores and dense structure may have limited cell ingrowth and thus tissue remodelling. 24,25,31 Previously, we reported on the controlled construction of an elastin and collagen-based conduit from scratch, containing highly-purified fibrillar type I collagen and elastin fibers. 32 This work demonstrated the compatibility of the constructed natural graft from a biochemical/biophysical, mechanical and haemocompatible point of view, with mechanical properties comparable to human tissue and not inducing platelet aggregation.…”

Section: Introductionmentioning

confidence: 99%

Vascular replacement using a layered elastin-collagen vascular graft in a porcine model: one week patency versus one month occlusion

et al. 2015

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ABSTRACT. A persistent clinical demand exists for a suitable arterial prosthesis. In this study, a vascular conduit mimicking the native 3-layered artery, and constructed from the extracellular matrix proteins type I collagen and elastin, was evaluated for its performance as a blood vessel equivalent.A tubular 3-layered graft (elastin-collagen-collagen) was prepared using highly purified type I collagen fibrils and elastin fibers, resembling the 3-layered native blood vessel architecture. The vascular graft was crosslinked and heparinised (37 § 4 mg heparin/mg graft), and evaluated as a vascular graft using a porcine bilateral iliac artery model. An intra-animal comparison with clinically-used heparinised ePTFE (Propaten Ò ) was made. Analyses included biochemical characterization, duplex scanning, (immuno)histochemistry and scanning electron microscopy.The tubular graft was easy to handle with adequate suturability. Implantation resulted in pulsating grafts without leakage. One week after implantation, both ePTFE and the natural acellular graft had 100% patencies on duplex scanning. ISSN: 1547-6278 print / 1555-8592 online DOI: 10.1080/15476278.2015 endothelium with a laminin-positive basal membrane layer). After one month, layered thrombi were found in the natural (4/4) and ePTFE graft (1/4), resulting in occlusion which in case of the natural graft is likely due to the porosity of the inner elastin layer. In vivo application of a molecularlydefined tubular graft, based on nature's matrix proteins, for vascular surgery is feasible.Organogenesis, 11:105-121, 2015 Copyright Ó Taylor & Francis Group, LLC

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“…Multilayered grafts that architecturally mimic the native vascular wall and mechanically match the gold standard of vessel replacement, saphenous vein, were produced. Han et al (2013) obtained multilayered vascular grafts from poly (ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) (PELCL), poly(L-lactide-co-glycolide) (PLGA), PCL and gelatin with sufficient mechanical properties for vascular tissue engineering applications. Yin et al (2013) developed vascular grafts from poly (L-lactide-co-ε-caprolactone) (PLLACL), collagen and chitosan at different blend ratios by electrospinning, also with similar mechanical properties to those of saphenous vein.…”

Section: Introductionmentioning

confidence: 99%

Mechanical behavior of bilayered small-diameter nanofibrous structures as biomimetic vascular grafts

Montini-Ballarin

Calvο

Caracciolo

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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a b s t r a c tTo these days, the production of a small diameter vascular graft (o6 mm) with an appropriate and permanent response is still challenging. The mismatch in the grafts mechanical properties is one of the principal causes of failure, therefore their complete mechanical characterization is fundamental. In this work the mechanical response of electrospun bilayered small-diameter vascular grafts made of two different bioresorbable synthetic polymers, segmented poly(ester urethane) and poly(L-lactic acid), that mimic the biomechanical characteristics of elastin and collagen is investigated. A J-shaped response when subjected to internal pressure was observed as a cause of the nanofibrous layered structure, and the materials used. Compliance values were in the order of natural coronary arteries and very close to the bypass gold standard-saphenous vein. The suture retention strength and burst pressure values were also in the range of natural vessels. Therefore, the bilayered vascular grafts presented here are very promising for future application as smalldiameter vessel replacements.

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Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF

Cited by 156 publications

References 46 publications

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Vascular replacement using a layered elastin-collagen vascular graft in a porcine model: one week patency versus one month occlusion

Mechanical behavior of bilayered small-diameter nanofibrous structures as biomimetic vascular grafts

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