Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro

Emter, Roger; Ellis, Graham; Natsch, Andreas

doi:10.1016/j.taap.2010.03.009

Cited by 292 publications

(210 citation statements)

References 44 publications

Supporting

Mentioning

204

Contrasting

Unclassified

Order By: Relevance

“…Partial cytotoxicity of a chemical may lead to a luciferase induction in dying cells and therefore chemicals are rated as sensitizers only when luciferase induction starts at non-cytotoxic concentrations. 42 For compound 2, >1.5-fold induction can only be noted at cytotoxic concentrations, and thus 2 is classified as a non-sensitizer by this method. The KeratinoSens™ results for both biaromatic compounds are in agreement with the LLNA data.…”

Section: Skin Sensitization Potency Studies: Llna and Keratinosens™mentioning

confidence: 99%

Analogues of the Epoxy Resin Monomer Diglycidyl Ether of Bisphenol F: Effects on Contact Allergenic Potency and Cytotoxicity

O’Boyle

Delaine

Luthman

et al. 2012

Chem. Res. Toxicol.

Self Cite

View full text Add to dashboard Cite

Diglycidyl ethers of bisphenol A (DGEBA) and bisphenol F (DGEBF) are widely used as components in epoxy resin thermosetting products. They are known to cause occupational and non-occupational allergic contact dermatitis. The aim of this study is to investigate analogues of DGEBF with regard to contact allergy and cytotoxicity. A comprehensive knowledge of the structural features that contribute to the allergenic and cytotoxic effects of DGEBF will guide the development of future novel epoxy resin systems with reduced health hazards for those coming into contact with them. It was found that the allergenic effects of DGEBF were dependent on its terminal epoxide groups. In contrast, it was found that the cytotoxicity in monolayer cell culture was not only dependent on the presence of epoxide groups, but also on other structural features.

show abstract

Section: Skin Sensitization Potency Studies: Llna and Keratinosens™mentioning

confidence: 99%

Analogues of the Epoxy Resin Monomer Diglycidyl Ether of Bisphenol F: Effects on Contact Allergenic Potency and Cytotoxicity

O’Boyle

Delaine

Luthman

et al. 2012

Chem. Res. Toxicol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other types of assays are in development and include peptide reactivity and single cellbased assays using, for example, human keratinocytes and dendritic cells (DC). They assess the activation of the innate immune system and of the antioxidant response which are hallmarks of the reactivity of such chemicals [16][17][18][19][20][21][22][23]. The innate immune response is essential for both the sensitization to the chemical and the subsequent adaptive T-cellmediated immune response upon further contacts [18][19][20].…”

Section: Replacement Of Animal Testing: T Cells As Tools In Immunotoxmentioning

confidence: 99%

“…The innate immune response is essential for both the sensitization to the chemical and the subsequent adaptive T-cellmediated immune response upon further contacts [18][19][20]. In the single cell-based assays the sensitization potential of chemicals is tested by measuring, for example, chemicalinduced antioxidant responses [21,22] or IL-18 production in keratinocytes [23] or the induction of activation markers such as CD86 on the cell surface of DC [16,17].…”

Section: Replacement Of Animal Testing: T Cells As Tools In Immunotoxmentioning

confidence: 99%

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

Martin

Esser

Schmucker

et al. 2010

Cell. Mol. Life Sci.

133

View full text Add to dashboard Cite

Chemicals can elicit T-cell-mediated diseases such as allergic contact dermatitis and adverse drug reactions. Therefore, testing of chemicals, drugs and protein allergens for hazard identification and risk assessment is essential in regulatory toxicology. The seventh amendment of the EU Cosmetics Directive now prohibits the testing of cosmetic ingredients in mice, guinea pigs and other animal species to assess their sensitizing potential. In addition, the EU Chemicals Directive REACh requires the retesting of more than 30,000 chemicals for different toxicological endpoints, including sensitization, requiring vast numbers of animals. Therefore, alternative methods are urgently needed to eventually replace animal testing. Here, we summarize the outcome of an expert meeting in Rome on 7 November 2009 on the development of T-cell-based in vitro assays as tools in immunotoxicology to identify hazardous chemicals and drugs. In addition, we provide an This article is dedicated to the memory of our colleague and friend Reinhard Wanner, died 2 April 2010. Cellular and Molecular Life Sciences overview of the development of the field over the last two decades.

show abstract

“…Response Element -Nuclear Factor Erythroid 2 (ARE-Nrf2) luciferase testing methods covered by KeratinoSens TM (Emter et al, 2010;Natsch et al, 2011) and the Human Cell 7…”

Section: Addressing the Development Of Efficient Toxicity Testing Strmentioning

confidence: 99%

“…Of these, one in chemico method and two in vitro methods were formally validated, i.e. the Direct Peptide Reactivity Assay (DPRA; (Gerberick et al, 2007)), the human Cell-Line Activation Test (h-CLAT; (Sakaguchi et al, 2006)) and the ARE-Nrf2 luciferase method covered by KeratinoSens TM (Emter et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

An economic approach to non-animal toxicity testing for skin sensitisation

Leontaridou¹

View full text Add to dashboard Cite

Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro

Cited by 292 publications

References 44 publications

Analogues of the Epoxy Resin Monomer Diglycidyl Ether of Bisphenol F: Effects on Contact Allergenic Potency and Cytotoxicity

Analogues of the Epoxy Resin Monomer Diglycidyl Ether of Bisphenol F: Effects on Contact Allergenic Potency and Cytotoxicity

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

An economic approach to non-animal toxicity testing for skin sensitisation

Contact Info

Product

Resources

About