Performance of electrolyte assays in the United Kingdom?reference target values

Thomas, M. A.; Smith, Stanley W.; Sall, Serigne Touba; Newsam, A. J.; Ducroq, David

doi:10.1007/s00769-003-0733-6

Cited by 2 publications

(2 citation statements)

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“…The two reference materials to be certified for electrolytes were analysed by the WEQAS reference laboratory using a published reference method in 2003 and 2005. 10 The samples had been stored at −70°C. All four reference materials were analysed for creatinine by LGC in 2003 and in 2006 using HPLC-IDMS.…”

Section: Resultsmentioning

confidence: 99%

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Production and certification of four frozen human serum certified reference materials containing creatinine and electrolytes

Barbagallo¹,

Boley²,

Holcombe³

et al. 2008

Ann Clin Biochem

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Background: This paper describes the preparation, analysis and certification of four frozen human serum certified reference materials (CRMs) containing creatinine and the electrolytes calcium, lithium, magnesium, potassium and sodium. These materials have been prepared to give concentrations of these analytes that cover the currently accepted analytical range. Methods: The analysis of the materials for certification purposes has been carried out using methodology traceable to primary standards, and which is acceptable as a reference method. The certification methods include liquid chromatography-mass spectrometry (LC-MS) with exact-matching isotope dilution calibration (EM-IDMS) for creatinine, inductively-coupled plasma optical emission spectroscopy (ICP-OES), ICP-MS and isotope-dilution inductively-coupled plasma mass spectroscopy (ID-ICP-MS) for the electrolytes. Results: The uncertainties estimated for these certified values include a component from the characterization measurements, as well as contributions from possible inhomogeneity and long-term instability. The certified values have been corroborated by measurements obtained in a major UK External Quality Assessment scheme, which have, with the exception of the determination of creatinine at a particularly low concentration, given excellent agreement. Conclusions: The materials are intended for use by pathology laboratories and manufacturers of in vitro diagnostic (IVD) kits for validation of existing routine methodology to a traceable standard, which will promote harmonization between the different methods, instruments and IVD kits used in these laboratories.

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Section: Resultsmentioning

confidence: 99%

“…10 The samples had been stored at 2708C. All four reference materials were analysed for creatinine by LGC in 2003 and in 2006 using HPLC-IDMS.…”

Section: Homogeneity and Stabilitymentioning

confidence: 99%