Performance of ELISA and Western blot to detect antibodies against HSV-2 using dried blood spots

García-Cisneros, Santa; Sánchez-Alemán, Miguel Ángel; Conde-Glez, Carlos J.; Lara-Zaragoza, Sergio J.; Herrera-Ortíz, Antonia; Plett-Torres, Tanya; Olamendi-Portugal, María

doi:10.1016/j.jiph.2018.10.007

Cited by 17 publications

(16 citation statements)

References 27 publications

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“…In addition, we measured the presence of HBV surface antigens in eluted DBS using ELISA (Murex HBsAg Version3, Diasonin S.p.A. UK Branch). As the dried blood samples contain lysed red blood cells, which can interfere with ELISA readout and give falsepositive results, we increased the cut-off values provided by the manufacturers of the ELISA kits by multiplying with a factor of 1.5 [17,18].…”

Section: Serologymentioning

confidence: 99%

“…Purified DNA from DCCS were assessed by a Taqman real-time PCR assay which identifies 12 high-risk (16,18,31,33,35,39,45,51,52, 56, 58 and 59) and two low-risk (6 and 11) types, by targeting segments of the E6/E7 region [20]. The amplification was performed in 8 duplex real-time PCR reactions, including the 14 HPV types and the human gene (the β-globin gene) which served as a control for sample quality and overall PCR efficiency.…”

Section: Hpv Typingmentioning

confidence: 99%

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Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in the Department of La Paz, Bolivia

et al. 2020

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Background: Bolivia has the highest prevalence of cervical cancer in South America and the prevalence of viral sexually transmitted infections (STIs) among people in urban cities is increasing. Little is known about the prevalence of viral STIs in rural communities, which generally have limited access to health care. In order to study the prevalence of viral STIs in rural Bolivia, we recruited women from villages and towns in the Department of La Paz in Bolivia. Methods: Three hundred ninety-four female participants were assessed for IgG-antibodies to herpes simplex virus type 2 (HSV-2), human immunodeficiency virus (HIV) and hepatitis B virus (HBV, anti-HBc), as well as for the presence of HBV surface antigen (HBsAg) in dried blood spots. The prevalence of 12 high-risk types of human papillomavirus (HPV) was assessed by qPCR in dried cervicovaginal cell spots from 376 of these women. χ 2 test was used to compare variables between the populations and binary logistic regression was used to identify risk factors associated with the positivity of the tests. Results: The seroprevalence of HSV-2 was 53% and of HBV 10.3%. HBAg was detected in 15.8% of women with anti-HBV antibodies indicating chronic infection. The frequency of high-risk HPV infection was 27%, with the most prevalent high-risk HPV types being HPV 56, 39 and 31 followed by HPV 16 and 18. Finally, none of the 394 women were seropositive for HIV, and about 64% of the studied population was positive for at least one of the viral infections. Conclusions: Women in Bolivian rural communities in La Paz show a high prevalence of HBV, HPV and, in particular, HSV-2. In contrast, none of the women were HIV positive, suggesting that the HIV prevalence in this population is low. The pattern of high-risk HPV types differed from many other countries with a predominance of HPV-types not included in the Gardasil vaccine which was officially introduced in Bolivia in April 2017.

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Section: Serologymentioning

confidence: 99%

Section: Hpv Typingmentioning

confidence: 99%

Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in the Department of La Paz, Bolivia

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In addition, we measured the presence of HBV surface antigens in eluted DBS using ELISA (Murex HBsAg Version3, Diasonin S.p.A. UK Branch). As the dried blood samples contain lysed red blood cells, which can interfere with ELISA readout and give false-positive results, we increased the cut-off values provided by the manufacturers of the ELISA kits by multiplying with a factor of 1.5 (17,18).…”

Section: Serologymentioning

confidence: 99%

“…Purified DNA from DCCS were assessed by a Taqman real-time PCR assay which identifies 12 high-risk (16,18,31,33,35,39,45…”

Section: Hpv Typingmentioning

confidence: 99%

Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in the Department of La Paz, Bolivia

Patzi-Churqui

Terrazas-Aranda

Liljeqvist

et al. 2020

Preprint

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Background: Bolivia has the highest prevalence of cervical cancer in South America and the prevalence of viral sexually transmitted infections (STIs) among people in urban cities is increasing. Little is known about the prevalence of viral STIs in rural communities, which generally have limited access to health care. In order to study the prevalence of viral STIs in rural Bolivia, we recruited women from villages and towns in the Department of La Paz in Bolivia. Methods: 394 female participants were assessed for IgG-antibodies to herpes simplex virus type 2 (HSV-2), human immunodeficiency virus (HIV) and hepatitis B virus (HBV, anti-HBc), as well as for the presence of HBV surface antigen (HBsAg) in dried blood spots. The prevalence of 12 high-risk types of human papillomavirus (HPV) was assessed by qPCR in dried cervicovaginal cell spots from 376 of these women. χ 2 test was used to compare variables between the populations and binary logistic regression was used to identify risk factors associated with the positivity of the tests. Results: The seroprevalence of HSV-2 was 53% and of HBV 10.3%. HBAg was detected in 15.8% of women with anti-HBV antibodies indicating chronic infection. The frequency of high-risk HPV infection was 27%, with the most prevalent high-risk HPV types being HPV 56, 39 and 31 followed by HPV 16 and 18. Finally, none of the 394 women were seropositive for HIV, and about 64% of the studied population was positive for at least one of the viral infections. Conclusions: Women in Bolivian rural communities in La Paz show a high prevalence of HBV, HPV and, in particular, HSV-2. In contrast, none of the women were HIV positive, suggesting that the HIV prevalence in this population is low. The pattern of high-risk HPV types differed from many other countries with a predominance of HPV-types not included in the Gardasil vaccine which was officially introduced in Bolivia in April 2017. Key words : Prevalence, Sexually transmitted infections, women, La Paz, Bolivia, rural communities, HSV-2, HIV, high-risk HPV, HBV.

show abstract

“…In addition, we measured the presence of HBV surface antigens in eluted DBS using ELISA (Murex HBsAg Version3, Diasonin S.p.A. UK Branch). As the dried blood samples contain lysed red blood cells, which can interfere with ELISA readout and give false-positive results, we increased the cut-off values provided by the manufacturers of the ELISA kits by multiplying with a factor of 1.5 [16,17].…”

Section: Serologymentioning

confidence: 99%

Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in Bolivia

Patzi-Churqui

Terrazas-Aranda

Liljeqvist

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Bolivia has the highest prevalence of cervical cancer in South America and the prevalence of viral sexually transmitted infections (STIs) among people in urban cities is increasing. Little is known about the prevalence of viral STIs in rural communities, which generally have limited access to health care. In order to study the prevalence of viral STIs in rural Bolivia, we recruited women from villages and towns in the Department of La Paz in Bolivia. Methods: 394 female participants were assessed for IgG-antibodies to herpes simplex virus type 2 (HSV-2), human immunodeficiency virus (HIV) and hepatitis B virus (HBV, anti-HBc), as well as for the presence of HBV surface antigen (HBsAg) in dried blood spots. The prevalence of 12 high-risk types of human papillomavirus (HPV) was assessed by qPCR in dried cervicovaginal cell spots from 376 of these women. χ 2 test was used to compare variables between the populations and binary logistic regression was used to identify risk factors associated with the positivity of the tests. Results: The seroprevalence of HSV-2 was 53% and of HBV 10.3%. HBAg was detected in 15.8% of women with anti-HBV antibodies indicating chronic infection. The frequency of high-risk HPV infection was 27%, with the most prevalent high-risk HPV types being HPV 56, 39 and 31 followed by HPV 16 and 18. Finally, none of the 394 women were seropositive for HIV, and about 64% of the studied population was positive for at least one of the viral infections. Conclusions: Women in Bolivian rural communities in La Paz show a high prevalence of HBV, HPV and, in particular, HSV-2, but a low prevalence of HIV. The pattern of high-risk HPV types differed from e.g. Sweden with a predominance of HPV-types not included in the Gardasil vaccine which was officially introduced in Bolivia in April 2017.

show abstract

Performance of ELISA and Western blot to detect antibodies against HSV-2 using dried blood spots

Cited by 17 publications

References 27 publications

Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in the Department of La Paz, Bolivia

Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in the Department of La Paz, Bolivia

Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in the Department of La Paz, Bolivia

Prevalence of viral sexually transmitted infections and HPV high-risk genotypes in women in rural communities in Bolivia

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