Performance of Five Agar Media for Recovery of Fungi from Isolator Blood Cultures

Procop, Gary W.; Cockerill, Franklin R.; Vetter, Emily A.; Harmsen, William S.; Hughes, John; Roberts, Glenn D.

doi:10.1128/jcm.38.10.3827-3829.2000

Cited by 15 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…e 30-day follow-up CT showed that the right lung cavity was reduced, and the left lung fibrous foci existed (d). [15]. Even with the newer diagnostic methods, such as hemolytic precipitation and culture of Candida, the positive rate is not high enough.…”

Section: Discussionmentioning

confidence: 99%

Imaging Characteristics of Invasive Pulmonary Fungal Infection Secondary to Hematological Diseases and Comparison before and after Treatment

Wang

Yang

Zhang

et al. 2021

Journal of Healthcare Engineering

View full text Add to dashboard Cite

Fungal infections have become crucial factors that threaten the prognosis and survival of blood disease patients. Here, we aim to analyze the epidemiological characteristics and early and advanced CT (computed tomography) manifestations of patients with invasive pulmonary fungal infections secondary to blood system diseases. 65 hospitalized patients from October 2018 to October 2020 with invasive pulmonary fungal infections secondary to blood diseases were enrolled. Blood diseases were recorded according to clinical and imaging data, and the serum galactomannan test (GM test) was conducted. Two senior radiologists analyzed the CT data and recorded the distribution of the lesions and CT signs. We analyzed and counted the first chest CT scan images of patients with nodule/mass type secondary to hematological diseases and invasive pulmonary fungal infection. The first CT nodules or mass-type lesions were statistically significant in nodule size, the number of lesions, distribution, and accompanying signs. Pulmonary fungal infection was common in both lungs during 7-day, 14-day, and 30-day follow-up CT. We also found that the nodular mass type was the main manifestation in the positive group of the GM test. Both the positive group and the negative group had the highest incidence of nodules. The incidence of air crescent signs in nodules or mass lesions in the positive group was higher than in the negative group, and the difference was statistically significant. To conclude, follow-up CT signs after antifungal treatment were highly sensitive to the early diagnosis of hematological diseases and secondary invasive pulmonary Eumycetes infection, which could be used for clinical treatment to provide help. GM test results were also related to CT manifestations such as air crescent sign, cavity, and halo sign.

show abstract

Section: Discussionmentioning

confidence: 99%

Imaging Characteristics of Invasive Pulmonary Fungal Infection Secondary to Hematological Diseases and Comparison before and after Treatment

Wang

Yang

Zhang

et al. 2021

Journal of Healthcare Engineering

View full text Add to dashboard Cite

show abstract

“…Furthermore, it can be difficult to determine whether a positive culture from a non‐sterile site is attributed to colonization or active infection (10). Nonetheless, improvements in culture techniques, such as the development of lysis centrifugation, new formulations of culture media, and automated blood culturing and monitoring systems, have led to a higher frequency and a more rapid detection of Candida species from blood samples (13–16). Unfortunately, sensitivity remains low, with the rate of positive samples from patients with invasive candidiasis at approximately 50% (17, 18).…”

Section: Traditional Methods Of Diagnosing Fungal Infectionsmentioning

confidence: 99%

Diagnostic challenges and recent advances in the early management of invasive fungal infections

Chandrasekar

2010

European J of Haematology

View full text Add to dashboard Cite

During the past 20 yr, the population of immunocompromized patients at risk of developing invasive fungal infections (IFIs) has increased, and there has been a shift in fungal epidemiology, with more infections caused by non‐Aspergillus molds and yeasts, which are often resistant to one or more antifungal drugs. Traditional diagnostic methods, such as culture and the histopathology of infected tissue, often fail to detect IFIs until the later stages. Furthermore, invasive diagnostic methods to obtain tissue may be contraindicated in severely ill patients; even when tissue is available, the morphology of several filamentous fungi is identical, or the cultures may fail to grow the pathogen. Recently developed non‐invasive diagnostic techniques, such as tests for serum markers and polymerase chain reaction assays, may allow for earlier and more accurate diagnoses – crucial in the effort to reduce morbidity and the risk of mortality. This article reviews current approaches to diagnosis and treatment, focusing on how an early and accurate diagnosis can guide treatment and improve outcomes. Strategies for improving the management of IFIs also are discussed.

show abstract

“…The sterile molten agar was poured inside the inoculated Petri dishes, swirled gently before allowing it to set. Culture plates were incubated at 25 o C for 72 hours [15]. Twenty-seven (27) yeasts were isolated from forty-three (n=43) milk samples cultured.…”

Section: Microbiological Analysis Of the Samples [14]mentioning

confidence: 99%

Molecular Characterization of Multi-Drug Resistant Fungal Isolates Obtained from Raw Cow Milk in Bowen University, Nigeria

Oluranti,

Majolagbe,

Elutade

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Cow milk is a highly nutritious food but many factors predispose it to microbial contamination. There is paucity of information on antifungal-resistant pathogens. Hence the study investigated the fungi from fresh cow milk samples and their resistance to some antifungals. Twenty-seven (27) fungi were isolated from forty-three (n=43) milk samples on PDA (Potato dextrose agar) media, re-cultured in glucose yeast extract broth, and incubated for 24 hours at 25°C. Taxonomic characterization on the isolates was done using photomicrography. Percentage occurrence of the isolates was determined. Molecular characterization was carried out on some isolates which were 100% resistant to antifungals; ketoconazole, amphotericin B, and clotrimazole using ITS1 and ITS4 primers. Isolate sequences were subjected to BLAST analysis and compared with representatives in GenBank. Kruskal-Wallis and Mann-Whitney Tests were used to analyze the data. Phylogenetic analysis and morphological characterization identified isolates as Rhodotorula paludigena, Candida sp and Candida tropicalis which had occurrence of 31.8%, 31.8% and 36.6% respectively. Level of resistance to ketoconazole (100%) and amphotericin B (92%) was significantly higher than clotrimazole (59%) (p< 0.05), while between ketoconazole and amphotericin B, there was no significant (p> 0.05) difference. Unprocessed raw milk is a potential source of drug-resistant pathogenic fungi. Pasteurization of raw milk is highly encouraged before consumption.

show abstract

Performance of Five Agar Media for Recovery of Fungi from Isolator Blood Cultures

Cited by 15 publications

References 33 publications

Imaging Characteristics of Invasive Pulmonary Fungal Infection Secondary to Hematological Diseases and Comparison before and after Treatment

Imaging Characteristics of Invasive Pulmonary Fungal Infection Secondary to Hematological Diseases and Comparison before and after Treatment

Diagnostic challenges and recent advances in the early management of invasive fungal infections

Molecular Characterization of Multi-Drug Resistant Fungal Isolates Obtained from Raw Cow Milk in Bowen University, Nigeria

Contact Info

Product

Resources

About