Performance of GeneXpert ultra in the diagnosis of Tuberculous Cervical lymphadenitis in formalin fixed paraffin embedded tissues

Arfaoui, A.; Benabdessalem, Chaouki; Ksentini, Meriem; Ferjani, Asma; Dekhil, Naira; Lahiani, Rim; Bchiri, Soumaya; Mardassi, Helmi; Barbouche, Mohamed‐Ridha; Boubake, Ilhem Boutiba Ben; Salah, Mamia Ben; Rammeh, Soumaya

doi:10.1016/j.tube.2020.102012

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…Therefore, the conclusions might be biased, which is not uncommon for this type of study design, and should be interpreted with caution and further validated elsewhere. Second, this laboratory protocol has a relatively high requirement for aseptic conditions, and the degree of sample grinding may affect the positive rate of tests due to the heterogeneous distribution of MTB in histological sections ( 29 ). Third, tissue sampling is an invasive procedure and the sample volume is limited, and thus the patient’s clinical conditions, financial burden, and laboratory testing capacity should be comprehensively considered before all three tests are recommended.…”

Section: Discussionmentioning

confidence: 99%

“…Third, tissue sampling is an invasive procedure and the sample volume is limited, and thus the patient’s clinical conditions, financial burden, and laboratory testing capacity should be comprehensively considered before all three tests are recommended. Finally, the GeneXpert MTB/RIF Ultra has been developed, based on GeneXpert MTB/RIF, to increase the positivity rate of MTB by using two additional multicopy targets ( IS6110 , IS1081 ) and four rpoB -specific probes ( 29 , 30 ). This was unavailable in low- and middle-income countries, including China.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study

Zhao

Zhang

et al. 2023

Microbiol Spectr

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study

Zhao

Zhang

et al. 2023

Microbiol Spectr

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“…Excision biopsy was performed to collect lymph nodes from CTL and CNTL patients. After an extensive diagnosis based on clinical symptoms, echography, histological, and bacteriological ( 29 ) arguments, patients were categorized into two groups: (i) CTL patients ( n = 27) with confirmed TB based on the presence of epithelioid granulomas and/or necrosis as well as positive culture and/or positive GeneXpert. (ii) CNTL patients ( n = 21).…”

Section: Methodsmentioning

confidence: 99%

HBHA-IGRA and cytotoxic mediators release assays for the diagnosis of cervical tuberculous lymphadenitis

Bchiri,

Bouzekri,

Ouni

et al. 2023

Microbiol Spectr

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Cervical tuberculous lymphadenitis (CTL), the most frequent extrapulmonary form of tuberculosis, is a serious health problem in Tunisia. CTL diagnosis is challenging mainly due to the paucibacillary nature of the disease and the potential misdiagnosis as cervical non-tuberculous lymphadenitis (CNTL). Here, we evaluated the performance of heparin-binding hemagglutinin (HBHA) interferon-gamma (IFN-γ) release assay (IGRA) for the diagnosis of CTL. In addition, we evaluated granzyme B, granulysin, and perforin release assays as CTL biomarkers and assessed their potential contribution to improve HBHA-IGRA performance. Peripheral blood mononuclear cells from CTL-suspected patients were stimulated with HBHA, early secreted antigenic target 6 (ESAT-6), or purified protein derivative (PPD) for 24 h in the presence of IL-7. Cytotoxic mediators and IFN-γ release were assessed by enzyme-linked immunosorbent assay. Receiver operating characteristic curves were used to evaluate the capacity of HBHA, ESAT-6, and PPD to discriminate between CTL ( n = 27) and CNTL ( n = 21). After applying bivariate and multivariate analyses, IFN-γ responses to HBHA appeared to offer the best distinction between CTL and CNTL, with an area under the curve of 0.9947, associated with 95.24% and 100% sensitivity and specificity, respectively. A principal component analysis showed clear clustering of the CTL versus the CNTL groups. This clustering was mainly attributed to HBHA-induced IFN-γ, PPD-induced granzyme B, and PPD-induced IFN-γ. These results thus suggest that the HBHA-IGRA provides high diagnostic accuracy for CTL versus CNTL, with high sensitivity and specificity. Combining HBHA-induced IFN-γ and PPD-induced granzyme B improves the accuracy to identify CTL. IMPORTANCE Cervical tuberculous lymphadenitis (CTL), the most frequent extrapulmonary form of tuberculosis, is currently a major health problem in Tunisia and in several regions around the world. CTL diagnosis is challenging mainly due to the paucibacillary nature of the disease and the potential misdiagnosis as cervical non-tuberculous lymphadenitis. This study demonstrates the added value of the heparin-binding hemagglutinin-interferon-gamma release assay as an immunoassay in the context of CTL.

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Pathology of Tuberculosis

Rammeh

2022

Imaging of Tuberculosis

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Performance of GeneXpert ultra in the diagnosis of Tuberculous Cervical lymphadenitis in formalin fixed paraffin embedded tissues

Cited by 8 publications

References 23 publications

Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study

Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study

HBHA-IGRA and cytotoxic mediators release assays for the diagnosis of cervical tuberculous lymphadenitis

Pathology of Tuberculosis

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