Performance of MALDI-TOF MS for identification of oral Prevotella species

Gürsoy, Mervi; Harju, Inka; Matomäki, Jaakko; Bryk, Anne; Könönen, Eija

doi:10.1016/j.anaerobe.2017.04.008

Cited by 21 publications

(17 citation statements)

References 38 publications

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“…The genus Prevotella comprises several species of Gram‐negative anaerobic bacilli commonly found in the human oral cavity. Some of the Prevotella species are clinically associated with oral infections . Of interest, P tannerae has not been associated with any of the classic bacterial complexes; however, later studies have recognized the participation of this species in chronic periodontitis .…”

Section: Discussionsupporting

confidence: 92%

Periodontal status, vascular reactivity, and platelet aggregation changes in rats submitted to hypercholesterolemic diet and periodontitis

Silva

Motta

Soares

et al. 2020

J of Periodontal Research

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Background and objectives: Periodontitis can corroborate with development and progression of atherosclerosis and a possible bidirectional interaction between both pathologies has been hypothesized. The aim of this work was to study the interactions between diet-induced hypercholesterolemia and ligature-induced periodontitis in Wistar rats submitted to both conditions. Material and methods: Animals were divided into four experimental groups: C (control: standard diet without periodontitis), Perio (periodontitis plus standard diet), HC (high cholesterol diet without periodontitis), and HC + Perio (high cholesterol diet plus periodontitis). The diets were offered for 45 days and a silk ligature was applied in the lower first molars of Perio and HC-Perio animals on day 34 and maintained for 11 days until euthanasia. The mandibles were excised, and alveolar bone loss was determined by macroscopic and micro-tomographic (µ-CT) imaging. Blood samples were obtained, and platelet aggregation was induced in plasma rich in platelets by adenosine diphosphate (ADP) and collagen. Endothelium-dependent vascular reactivity and protein expression of endothelial (eNOS), phosphorylated endothelial (peNOS), and inducible (iNOS) nitric oxide synthases were evaluated in aorta samples. Results:The HC diet combined with periodontitis (HC + Perio group) was associated with an increased alveolar bone loss, when compared to the other groups. Both in Perio and HC groups, platelet aggregation induced by ADP or collagen was increased, while maximum aortic relaxation induced by acetylcholine was decreased.Periodontitis or HC diet alone decreased the expression of peNOS and HC diet increased the expression of iNOS. In contrast, no additive or synergistic effects were found in vascular reactivity or in platelet aggregation when the two conditions were associated (HC + Perio group). Conclusion:Hypercholesterolemia accelerated the process of bone loss induced by periodontitis while a high cholesterol diet or periodontitis individually increased

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Section: Discussionsupporting

confidence: 92%

Periodontal status, vascular reactivity, and platelet aggregation changes in rats submitted to hypercholesterolemic diet and periodontitis

Silva

Motta

Soares

et al. 2020

J of Periodontal Research

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“…16 The advantages of MALDI-TOF technology are speed, accuracy, and low running costs and it has therefore been widely used for microbiological diagnostics of oral microbiota in human medicine in the last decade. 17,18 Reliability of results is highly dependent on the quality of the associated database, which is becoming increasingly extensive in the veterinary field. 19 Despite published evidence in human literature relating certain features of endodontic microbiota with distinct forms of endodontic disease, 5 we were unable to find any association between the number of bacterial species involved in the root canal infection and clinical or radiographic signs of endodontic disease.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Microbiological Aspects of Naturally Occurring Primary Endodontic Infections in Dogs

et al. 2019

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Dental fractures are common in dogs, but data on microbiology of naturally occurring primary endodontic infections, and their relation to clinical and radiographic signs, are lacking. Samples were obtained from root canals of 32 periodontally healthy fractured teeth under aseptic conditions and immediately cultured for aerobic and anaerobic bacteria. Cultures were further identified by matrix-assisted laser desorption/ionization, time of flight technology. Sixty-one bacteria (30 bacterial species) were isolated from root canals; 54% were Gram-negative bacteria, 53% were facultative anaerobic, and 42% were anaerobic bacteria. Number of bacterial species in the root canals declined with the duration of fractures over 12 months. No statistically significant association was found between the number of bacterial species involved in the root canal infection and any of the clinical or radiographic signs of endodontic disease. Naturally occurring primary endodontic infections in dogs appear to be polymicrobial and involve only a selected number of opportunistic pathogen species.

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“…The data were analyzed by the MALDI Biotyper RTC 3.0 software (Bruker Daltonics). Equipment parameters were adjusted according to the manufacturer's recommendations …”

Section: Methodsmentioning

confidence: 99%

“…Then, the pools were covered with 1 μL of the matrix (α-cyano- 24,25 The sample size was considered with a power of 90% and a significance level of .05 (two-tailed) for Pseudomonas frequencies of 80% and 20%, supported on a preceding investigation in implants restored with screwed and cemented reconstructions, correspondingly. 26 With this information, it was established that ≥15 implants per group would be indispensable.…”

Section: Microbial Samplingmentioning

confidence: 99%

Gram‐negative enteric rods/Pseudomonas colonization in mucositis and peri‐implantitis of implants restored with cemented and screwed reconstructions: A cross‐sectional study

Ramón-Morales

Medina

2019

Clin Implant Dent Rel Res

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Purpose To evaluate the association between Gram‐negative enteric rods and Pseudomonas with mucositis and peri‐implantitis, in implants restored with cemented and screw‐retained reconstructions. Materials and Methods Individuals treated with implants, diagnosed with peri‐implant mucositis and or peri‐implantitis, participated in this study. Microbiota from the implant and the gingival sulcus of two neighboring teeth were also studied. The microorganisms were identified using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Results A total of 52 and 51 implants cemented and screwed restored were evaluated in 31 patients. The presence of Gram‐negative enteric rods/Pseudomonas (P = .01) was significantly higher in the cemented restored implants. When the unit of analysis was the implant, the presence of Gram‐negative enteric rods/Pseudomonas was associated with cemented restored implants after adjustment for possible confounders (odds ratio [OR] = 3.7; 95% CI, 1.4‐10; P = .01); besides, peri‐implantitis was statistically significant in the multivariate model (P = .008). Considering the patient as a unit of analysis, peri‐implantitis was associated with cemented restored implants (OR = 9.4; 95% CI, 2‐46; P = .006); this statistically significant association remained after adjusting for potential confounders (OR = 6.8; 95% CI, 1.3‐37; P = .02). Conclusion The presence of Gram‐negative enteric rods/Pseudomonas was associated with cemented restored implants; besides, peri‐implantitis was associated with cemented restored implants.

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Performance of MALDI-TOF MS for identification of oral Prevotella species

Cited by 21 publications

References 38 publications

Periodontal status, vascular reactivity, and platelet aggregation changes in rats submitted to hypercholesterolemic diet and periodontitis

Periodontal status, vascular reactivity, and platelet aggregation changes in rats submitted to hypercholesterolemic diet and periodontitis

Microbiological Aspects of Naturally Occurring Primary Endodontic Infections in Dogs

Gram‐negative enteric rods/Pseudomonas colonization in mucositis and peri‐implantitis of implants restored with cemented and screwed reconstructions: A cross‐sectional study

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