Performance of mesophilic biohydrogen-producing cultures at thermophilic conditions

Gupta, Madhulika A.; Gomez-Flores, Maritza; Nasr, Noha; Elbeshbishy, Elsayed; Hafez, Hisham; Naggar, M. Hesham El; Nakhla, George

doi:10.1016/j.biortech.2015.06.047

Cited by 22 publications

(4 citation statements)

References 26 publications

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“…For example, Holwerda and Lynd (2013) found that the best fit to their results on C. thermocellum was with a substrate utilization rate that is both first order with respect to substrate and first order in cells. Recently, Gupta et al (2015) found a µ max of 0.05 d −1 on cellulose using mesophilic anaerobic digested sludge (ADS) and a Ks of 2.1 g l −1 , which is four times lower than that achieved by C. termitidis in the present study.…”

Section: Discussioncontrasting

confidence: 67%

Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose

2017

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Cellulose utilization by hydrogen producers remains an issue due to the low hydrogen yields reported and the pretreatment of cellulose prior to fermentation requires complex and expensive steps. Clostridium termitidis is able to breakdown cellulose into glucose and produce hydrogen. On the other hand, Clostridium beijerinckii is not able to degrade cellulose but is adept at hydrogen production from glucose; therefore, it was chosen to potentially enhance hydrogen production when co-cultured with C. termitidis on cellulose. In this study, batch fermentation tests were conducted to investigate the direct hydrogen production enhancement of mesophilic cellulolytic bacteria C. termitidis co-cultured with mesophilic hydrogen producer C. beijerinckii on cellulose at 2 g l−1 compared to C. termitidis mono-culture. Microbial kinetics parameters were determined by modeling in MATLAB. The achieved highest hydrogen yield was 1.92 mol hydrogen mol−1 hexose equivalentadded in the co-culture compared to 1.45 mol hydrogen mol−1 hexose equivalentadded in the mono-culture. The maximum hydrogen production rate of 26 ml d−1 was achieved in the co-culture. Co-culture exhibited an overall 32 % enhancement of hydrogen yield based on hexose equivalent added and 15 % more substrate utilization. The main metabolites were acetate, ethanol, lactate, and formate in the mono-culture, with also butyrate in the co-culture. Additionally, the hydrogen yield of C. beijerinckii only in glucose was 2.54 mol hydrogen mol−1 hexose equivalent. This study has proved the viability of co-culture of C. termitidis with C. beijerinckii for hydrogen production directly from a complex substrate like cellulose under mesophilic conditions.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0256-2) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 67%

Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose

2017

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“…The reliability of these data was demonstrated by the fact that COD balance was maintained at 90% during dark fermentation. 29 At the end of dark fermentation for bioH 2 production, the final pH dropped to 4.8−5.0 in all reactors. This small change in pH provided opportunities for bioH 2 production bacteria to acclimate to the new environment, had few suppressive effects on the microorganisms, and did not limit bioH 2 production in this study.…”

Section: Resultsmentioning

confidence: 93%

“…Its lag time range for fermentation was 5.98–6.48 h. However, there was an adaptation phase for the microorganisms as the HA concentration increased, and there was no significant change in the lag time of the dark fermentation bioH 2 production system. The reliability of these data was demonstrated by the fact that COD balance was maintained at 90% during dark fermentation . At the end of dark fermentation for bioH 2 production, the final pH dropped to 4.8–5.0 in all reactors.…”

Section: Resultsmentioning

confidence: 99%

Hydroxyapatite Fabrication for Enhancing Biohydrogen Production from Glucose Dark Fermentation

Wang

et al. 2022

ACS Omega

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Hydroxyapatite (HA) had the effect of maintaining the pH balance of the reaction system and promoting enzyme activity. In this work, hydroxyapatite was synthesized by coprecipitation and characterized for biohydrogen (bioH 2 ) production from glucose. The highest bioH 2 yield obtained was 182.33 ± 2.41 mL/g glucose, amended with an optimal dosage of 400 mg/L HA, which was a 55.80% higher bioH 2 yield compared with the control group without any addition. The results indicated that HA facilitated the deterioration of organic substances and increased the concentration of soluble microbial products (SMPs). Microbial community analysis revealed that HA significantly increased the abundance of Firmicutes from 35.27% (0 mg/L, HA) to 76.41% (400 mg/L, HA), which played an essential role in bioH 2 generation. In particular, the abundance of Clostridium sensu stricto 1 increased from 15.33% (0 mg/L HA) to 45.17% (400 mg/L HA) and became the dominant bacteria. The results also indicated that HA likely improves bioH 2 production from organic wastewater in practice.

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“…The experimental design was organised as a 2 × 3 (2 times 3) factorial and examined the main effects and interaction of two factors (incubation temperature, 35 °C or 55 °C, quantitative factor, and diaper conditioning, qualitative or categorical factor) on H 2 production in the batch fermentation of used (waste) disposable diapers (Montgomery, 2012). Temperatures 35 °C and 55 °C were chosen based on this rationale: Typical optimal ranges for anaerobic fermentations are mesophilic (34 °C–37 °C) and thermophilic (52 °C–57 °C) (see Gupta et al, 2015; Valdez-Vázquez and Poggi-Varaldo, 2009a for temperature effects on anaerobic H 2 fermentations). Intermediate temperatures (such as 45 °C Fair and Moore, 1937; Fair et al, 1968) as well as lower temperatures, generally give lower results (Fair et al, 1968; Madigan et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Biohydrogen production from used diapers: Evaluation of effect of temperature and substrate conditioning

Sotelo-Navarro¹,

Poggi-Varaldo

Turpin-Marion³

et al. 2017

Waste Manag Res

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This research assessed the viability to use disposable diapers as a substrate for the production of biohydrogen, a valuable clean-energy source. The important content of cellulose of disposable diapers indicates that this waste could be an attractive substrate for biofuel production. Two incubation temperatures (35 °C and 55 °C) and three diaper conditioning methods (whole diapers with faeces, urine, and plastics, WD; diapers without plastic components, with urine and faeces, DWP; diapers with urine but without faeces and plastic, MSD) were tested in batch bioreactors. The bioreactors were operated in the solid substrate anaerobic hydrogenogenic fermentation with intermittent venting mode (SSAHF-IV). The batch reactors were loaded with the substrate at ca. 25% of total solids and 10% w/w inoculum. The average cumulative bioH production followed the order WD > MSD > DWP. The bio-H production using MSD was unexpectedly higher than DWP; the presence of plastics in the first was expected to be associated to lower degradability and H2 yield. BioH production at 55 °C was superior to that of 35 °C, probably owing to a more rapid microbial metabolism in the thermophilic regime. The results of this work showed low yields in the production of H at both temperatures compared with those reported in the literature for municipal and agricultural organic waste. The studied process could improve the ability to dispose of this residue with H generation as the value-added product. Research is ongoing to increase the yield of biohydrogen production from waste disposable diapers.

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Performance of mesophilic biohydrogen-producing cultures at thermophilic conditions

Cited by 22 publications

References 26 publications

Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose

Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose

Hydroxyapatite Fabrication for Enhancing Biohydrogen Production from Glucose Dark Fermentation

Biohydrogen production from used diapers: Evaluation of effect of temperature and substrate conditioning

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