Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture

Abstract

ABSTRACT. To develop new ways to breed peanut, we irradiated seeds of the Luhua 11 cultivar with a mixed high-energy particle field at different doses. The embryonic leaflets were extracted as explants and incubated on somatic embryo induction medium and then on somatic embryo germination and regeneration medium. After being grafted, the M 1 -generation plants were transplanted, and seeds from each M 1 -generation plant were harvested. In the following year, the M 2 -generation seeds were planted separately. Some M 2 -generation plants showed distinct character segregation relative to the mutagenic parent in terms of vigor, fertility, plant height, branch number, and pod size and shape. M 2 -generation plants that had a high pod weight per plant tended to produce M 3 -generation offspring that also had a high pod weight per plant, much higher than that of the mutagenic parent, Luhua 11. M 4 -10838 J.S. Wang et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 10837-10848 (2015) generation seeds varied greatly in quality, and 35 individuals with an increased fat content (>55%) were obtained. Overall, the results indicate that the combination of mutagenesis via mixed high-energy particle field exposure and tissue culture is promising for peanut breeding.

Study on the multiple characteristics of M3 generation of pea mutants obtained by neutron irradiation

“…Since then, scientists worldwide have studied radiation mutation breeding and the biological effects caused by radiation. Much research has been conducted in the last decade [3][4][5][6][7][8][9][10][11]. According to statistical data from the FAO and International Atomic Energy Agency (IAEA), a total of 3,322 plant mutant species have been developed around the world as of March 2020 [12].…”

Analysis of Morphological Features and Flavonoid Compositions of Crabapple Exposed to 60Coγ Radiation Treatments

“…Moreover, in attempts to improve seed yield, some studies screen out superior populations of faba beans (Vicia faba L.) in terms of plant height, number of flowers, fresh weight, dry weight, root length, nodule number, root fresh weight and root dry weight (Backouchi et al, 2015). Some investigators irradiated the seeds of the 'Luhua 11' cultivar with a mixed high-energy particle field at different doses, and then they developed new ways to breed peanuts by observing the vigor, fertility, plant height, branch number, and pod size and shape of M2-generation plants (Wang et al, 2015). Thus, for a variety of perennial horticultural plants, the external shape and internal structure of the plant are widely used during resource identification and selection of breeding materials as the primary morphological markers that indicate mutations caused by radiation.…”