Performance of pigs with or without the intestinal receptor for<i>Escherichia coli</i>K88

Edfors-Lilja, Inger; Petersson, H; Gahne, B.

doi:10.1017/s000335610001816x

Cited by 36 publications

(33 citation statements)

References 19 publications

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“…They performed the adhesion assay by incubating single enterocytes with bacteria as described by Edfors-Lilja et al (1986). Ten to 20 epithelial cells were examined for adhesion by interference contrast microscopy.…”

Section: Resultsmentioning

confidence: 99%

Screening of pigs resistant to F4 enterotoxigenic Escherichia coli (ETEC) infection

Rasschaert

Verdonck

Goddeeris

et al. 2007

Veterinary Microbiology

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The present study analysed quantitatively the mucin 4 polymorphism for determing the F4ac/ab receptor status of a total of 63 pigs by comparing it with the in vitro villous adhesion assay. The probability of a susceptible genotype for the mucin 4 increases significantly with increasing F4ab or F4ac ETEC adhesion per 250 mm villi (P = 0.029 for F4ab, P = 0.030 for F4ac), with the odds ratio for each unit increase of F4ab or F4ac equal to, respectively, 1.036 (95% CI [1.004-1.069]) and 1.018 (95% CI [1.002-1.034]). In the phenotypic in vitro villous adhesion test, a cut-off value of 5 bacteria was chosen as a criteria for the distinction between an F4R positive and F4R negative pig. The sensitivity and specificity for the in vitro villous adhesion test, with the genotyping test for mucin 4 as golden standard, is 100% and 24%, respectively, for F4ab as well as F4ac. Absence of adhesion of F4ac and F4ab ETEC to the villous brush borders was not associated with genotypic resistance suggesting that there is at least one other receptor for F4ab/ac Escherichia coli. As a consequence, not only mucin 4 gene polymorphism but also expression of these other receptor(s) has to be included in a screening assay for F4ac/ab receptor negative pigs. #

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Section: Resultsmentioning

confidence: 99%

Screening of pigs resistant to F4 enterotoxigenic Escherichia coli (ETEC) infection

Rasschaert

Verdonck

Goddeeris

et al. 2007

Veterinary Microbiology

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“…High costs, laboriousness and the fact that pigs have to be slaughtered and, therefore, cannot be used for breeding purposes are serious drawbacks for the common adhesion tests to be used on large scale, as is also discussed for selection on E. coli-F18 resistance [30,31]. Moreover, it is debatable whether it is advisable to breed out a trait that might have an unknown beneficial function [32,33] or that will change the selection pressure on pathogenic E. coli. We calculated that, assuming that the transmission from F4Rx piglets to other piglets is 0, the maximum fraction of F4R+ piglets should be 0 .…”

Section: Discussionmentioning

confidence: 99%

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Geenen

Meulen²,

Bouma

et al. 2004

Epidemiol. Infect.

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SUMMARY F4+Escherichia coli is an important agent of post-weaning diarrhoea in piglets. Piglets that express an adhesion site for F4+ E. coli in their small intestine (F4R+) shed higher numbers of F4+ E. coli than piglets lacking this site (F4Rx). We hypothesized that F4R+ piglets are more infectious and more susceptible for F4+ E. coli. This implies that in populations with F4R+ and F4Rx piglets, the transmission would be dependent on the frequency of both types of animals. To quantify the difference in infectiousness and susceptibility, a one-to-one transmission experiment was performed with 20 pairs consisting of one inoculated and one contact piglet. Based on the contact infections observed, transmission parameters were estimated with generalized linear models. F4R+ piglets were infectious for other piglets and the reproduction ratio (R 0 ) for homogeneous F4R+ populations, that is the average number of secondary infections that one F4R+ pig will cause during its entire infectious period in a population of susceptible F4R+ individuals only, was estimated as 7 . 1. F4R+ piglets were more susceptible than F4Rx piglets and reducing the fraction of F4R+ piglets of a population will reduce transmission. It was calculated that in order to prevent major outbreaks of F4+ E. coli (R 0 <1), the fraction of F4R+ piglets must be lower than 0 . 14.

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“…Huang (1977) found no evidence of an association between early growth in pigs and ability to develop humoral immune response to synthetic antigens. Higher gains and feed efficiency were found by Edfors-Lilja et al (1986) to be related to susceptibility to K88 E. coil scours. Correlations of production traits and immune response to PR and B. bronchiseptica vaccines from this study are shown in table 6.…”

Section: Resultsmentioning

confidence: 89%

“…A primary component of disease resistance is the humoral immune response (Gavora and Spencer, 1983 ;Buschmann et al, 1985;Warner et al, 1987 (Buschman et al, 1974), hen egg white lysozyme (Vaiman et al, 1978), E. coli vaccine (Edfors-Lilja et al, 1984, 1985, Bordetella bronchiseptica vaccine (Rothschild et al, 1984a) and pseudorabies vaccine (Rothschild et al, 1984b). Little is known about the relationships among immune response traits and production traits (Rothschild, 1985;Edfors-Lilja et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Genetic Control of Immune Response to Pseudorabies and Atrophic Rhinitis Vaccines: I. Heterosis, General Combining Ability and Relationship to Growth and Backfat

Meeker¹,

Rothschild²,

Christian³

et al. 1987

Journal of Animal Science

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Data from 988 pigs from 119 litters farrowed in two seasons of a three-breed diallel crossbreeding experiment were analyzed to estimate general combining abilities of breeds and heterosis for humoral immune response to pseudorabies virus and atrophic rhinitis vaccines. Twenty purebred boars and 85 sows of the Duroc, Landrace and Yorkshire breeds were mated to provide the nine breed-of-sire and breed-of-dam combinations. Immune response was measured after vaccination. A modified-live pseudorabies virus (PR) vaccine was administered to piglets at 28 d of age and response measured as log 2 serum neutralization titers at 56 d. An inactivated B. bronchiseptica bacterin was administered at 28, 42 and 112 d. Antibody levels were measured relative to positive and negative controls at 28, 56 and 119 d by using an enzyme-linked immunosorbent assay. The results of this study showed that ranking by breed of sire and breed of dam did not differ for general combining ability, and no evidence of significant heterosis for any immune responses was observed. Higher immune response at 56 d to B. bronchiseptica vaccine was associated with lower weaning weight (r = −.09, P<.01). Correlations of days to 100 kg with 56-d and 119-d B. bronchiseptica antibody levels were .15 (P<.01) and .12 (P<.01). The relationship between humoral immune response to PR vaccine and growth traits was similar to that observed for B. bronchiseptica vaccine. Immune response to both antigens was not associated with backfat thickness. Further research using more specifically defined antigens and homogeneous populations of animals is needed to examine nonadditive gene action on the humoral immune response in swine. Iowa State University, Ames 50011 ABSTRACTData from 988 pigs from 119 litters farrowed in two seasons of a three-breed diallel crossbreeding experiment were analyzed to estimate general combining abilities of breeds and heterosis for humoral immune response to pseudorabies virus and atrophic rhinitis vaccines. Twenty purebred boars and 85 sows of the Duroc, Landrace and Yorkshire breeds were mated to provide the nine breed-of-sire and breed-of-dam combinations. Immune response was measured after vaccination. A modified-live pseudorabies virus (PR) vaccine was administered to piglets at 28 d of age and response measured as log 2 serum neutralization titers at 56 d. An inactivated B. bronchiseptica bacterin was administered at 28, 42 and 112 d. Antibody levels were measured relative to positive and negative controls at 28, 56 and 119 d by using an enzyme-linked immunosorbent assay. The results of this study showed that ranking by breed of sire and breed of dam did not differ for general combining ability, and no evidence of significant heterosis for any immune responses was observed. Higher immune response at 56 d to B. bronchiseptica vaccine was associated with lower weaning weight (r = -.09, P<.01). Correlations of days to 100 kg with 56-d and 119-d B. bronchiseptica antibody levels were .15 (P<.01) and .12 (P<.01). The relationship between ...

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Performance of pigs with or without the intestinal receptor forEscherichia coliK88

Cited by 36 publications

References 19 publications

Screening of pigs resistant to F4 enterotoxigenic Escherichia coli (ETEC) infection

Screening of pigs resistant to F4 enterotoxigenic Escherichia coli (ETEC) infection

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Genetic Control of Immune Response to Pseudorabies and Atrophic Rhinitis Vaccines: I. Heterosis, General Combining Ability and Relationship to Growth and Backfat

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