Performance of rapid immunochromatographic assay in the diagnosis of Trichomoniasis vaginalis

Hegazy, Mamdouh M.; El-Tantawy, Nora; Soliman, Mosaad; El-Sadeek, Ehaab S.; El-Nagar, Hassan S.

doi:10.1016/j.diagmicrobio.2012.05.003

Cited by 43 publications

(34 citation statements)

References 38 publications

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“…27 28 The GeneXpert, already shown to be highly accurate, 14 15 is now used routinely in a number of non-POCT settings. 16 The extra TV diagnosis is consistent with other reports of high OSOM TV test sensitivity, 17 18 29 whereas the six BV cases that were diagnosed by the POCT alone, and consequently treated, are more difficult to interpret as the few performance evaluations of VS-SENSE use pH paper as the reference standard, 21 22 and results should be interpreted together with clinical examination. 20 In our service evaluation, it would appear that in some instances, the VS-SENSE BV POCT was used as a diagnostic tool as opposed to as a diagnostic aid, as had been instructed.…”

Section: Discussionsupporting

confidence: 81%

“…16 OSOM TV has reported sensitivities of 88.2%-98.0% and specificities of 99.4%-100% compared with wet mount microscopy, culture or a composite gold standard including PCR assays. [17][18][19] The Alere VS-SENSE BV test, an acidity test based on pH levels designed to be used together with additional clinical data/indications such as Amsel or Hay-Ison criteria or Nugent Gram stain, 20 has reported sensitivities of 82.3%-91% and specificities of 94.2%-97.8% compared with pH paper. 21 22 On the basis of the tests' intention for use, clinicians were informed to use the GeneXpert CT/NG and OSOM TV tests as diagnostic tests with definitive results.…”

Section: Introductionmentioning

confidence: 99%

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Impact of deploying multiple point-of-care tests with a ‘sample first’ approach on a sexual health clinical care pathway. A service evaluation

et al. 2017

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ObjectivesTo assess clinical service value of STI point-of-care test (POCT) use in a ‘sample first’ clinical pathway (patients providing samples on arrival at clinic, before clinician consultation). Specific outcomes were: patient acceptability; whether a rapid nucleic acid amplification test (NAAT) for Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) could be used as a POCT in practice; feasibility of non-NAAT POCT implementation for Trichomonas vaginalis (TV) and bacterial vaginosis (BV); impact on patient diagnosis and treatment.MethodsService evaluation in a south London sexual health clinic. Symptomatic female and male patients and sexual contacts of CT/NG-positive individuals provided samples for diagnostic testing on clinic arrival, prior to clinical consultation. Tests included routine culture and microscopy; CT/NG (GeneXpert) NAAT; non-NAAT POCTs for TV and BV.ResultsAll 70 (35 males, 35 females) patients approached participated. The ‘sample first’ pathway was acceptable, with >90% reporting they were happy to give samples on arrival and receive results in the same visit. Non-NAAT POCT results were available for all patients prior to leaving clinic; rapid CT/NG results were available for only 21.4% (15/70; 5 males, 10 females) of patients prior to leaving clinic. Known negative CT/NG results led to two females avoiding presumptive treatment, and one male receiving treatment directed at possible Mycoplasma genitalium infection causing non-gonococcal urethritis. Non-NAAT POCTs detected more positives than routine microscopy (TV 3 vs 2; BV 24 vs 7), resulting in more patients receiving treatment.ConclusionsA ‘sample first’ clinical pathway to enable multiple POCT use was acceptable to patients and feasible in a busy sexual health clinic, but rapid CT/NG processing time was too long to enable POCT use. There is need for further development to improve test processing times to enable POC use of rapid NAATs.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Impact of deploying multiple point-of-care tests with a ‘sample first’ approach on a sexual health clinical care pathway. A service evaluation

et al. 2017

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“…vaginalis is one of the common causes for genital discharge. Wet mount evaluation and modified Diamond's base are routinely used for diagnosis, and higher sensitivity was detected for the latter test method [26]. A wide range of presence was reported (1%-23.3%) [20,21].…”

Section: Discussionmentioning

confidence: 99%

Comparison of cervico-vaginal colonization among sexually active women by intrauterine device use

Dağlı

Demir

2015

J Infect Dev Ctries

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Introduction: In this study we aimed to evaluate the effect of intrauterine devices (IUDs) on cervico-vaginal colonization. Methodology: Cervico-vaginal samples of 96 patients with vaginal discharge were included in the study. Microscopic evaluation, bacteriological and fungal culture, and antigen testing for Chlamydia trachomatis using an immunochromatographic test method were performed. Results: Trichomonas vaginalis was not detected by wet mount examination. Gram smear revealed that seven patients (7.3%) had Candida spp. and five (5.2%) had clue cell. Of the 96 swabs tested for conventional culture, pathogenic microorganisms were isolated from 24 patients. While Neisseria gonorrhoeae was not found in any of the sample, five (5.2%) were positive for Gardnerella vaginalis. Five (5.2%) were positive for C. trachomatis antigen, while three positivity only for C. trachomatis antigen, one had G. vaginalis additionally, and the other had a mixed infection. Chlamydial antigen positivity was higher among women over 30 years of age (p = 0.157). Increase in polymorphonuclear leukocytes (PNL) was detected 40% and 35.2% of samples, positive and negative, for chlamydial antigen, respectively (p = 1.000). Among IUD+ cases, increase in PNL, fungal elements, E. coli and Gram-positive bacteria and decrease in Lactobacillus spp. were observed, compared to IUD-cases. No statistically significant relationship was detected between IUD and chlamydial antigen with the reported rates of 4.8% and 5.6% for IUD+ or IUD-, respectively (p > 0.05). Conclusion: Statistically significant relationship was not detected between IUD and cervico-vaginal colonization. More comprehensive studies using specific test methods should be conducted to better understand the relationship.

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“…29,30 The sensitivity and specificity has been reported to be 80-94% and greater than 95%, respectively, depending on the comparator. 27,28,34,35 This test requires no instrumentation and provides a result within 30 min and is a suitable alternative to culture or molecular testing. Although these tests are more sensitive than those requiring vaginal wet preparation, false positives might occur, especially in populations with a low prevalence of disease, so consideration should be given to confirming positives in that situation.…”

Section: Point Of Care Tests Level Of Evidence: Iib Bmentioning

confidence: 99%

United Kingdom National Guideline on the Management of Trichomonas vaginalis 2014

et al. 2014

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Performance of rapid immunochromatographic assay in the diagnosis of Trichomoniasis vaginalis

Cited by 43 publications

References 38 publications

Impact of deploying multiple point-of-care tests with a ‘sample first’ approach on a sexual health clinical care pathway. A service evaluation

Impact of deploying multiple point-of-care tests with a ‘sample first’ approach on a sexual health clinical care pathway. A service evaluation

Comparison of cervico-vaginal colonization among sexually active women by intrauterine device use

United Kingdom National Guideline on the Management of Trichomonas vaginalis 2014

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