Performance of the BacT/Alert Virtuo Microbial Detection System for the culture of sterile body fluids: prospective multicentre study

She, Rosemary C.; Romney, Marc G.; Jang, Willson; Walker, Tamar; Karichu, James; Richter, Sandra

doi:10.1016/j.cmi.2017.12.011

Cited by 16 publications

(6 citation statements)

References 21 publications

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“…In this study, the bile samples were incubated and separated by a BacT/Alert3D automated blood culture instrument [ 12 ] in accordance with international standard operation procedures for bacterial detection [ 13 , 14 ]; this method can guarantee the growth and accurate identification of specific organisms, even if the samples contained multiple species [ 13 ]. According to the data obtained from bile specimens from 560 AP patients in hospitals, 120 positive cases and 132 strains of pathogenic bacteria were identified, with a positive infection rate of 21.43%, which was lower than that obtained in an analysis without stratification by sample type [ 15 ], similar to those in blood culture specimens in some hospitals in northern China without disease classification [ 16 ], and higher than those in blood culture specimens in some hospitals in central cities of China [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of and risk factors for biliary pathogen infection in patients with acute pancreatitis

et al. 2021

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Background Infection in patients with acute pancreatitis, especially severe acute pancreatitis patients, is a common and important phenomenon, and the distributions and drug resistance profiles of bacteria causing biliary infection and related risk factors are dynamic. We conducted this study to explore the characteristics of and risk factors for bacterial infection in the biliary tract to understand antimicrobial susceptibility, promote the rational use of antibiotics, control multidrug-resistant bacterial infections and provide guidance for the treatment of acute pancreatitis caused by drug-resistant bacteria. Methods The distribution of 132 strains of biliary pathogenic bacteria in patients with acute pancreatitis from January 2016 to December 2020 were analyzed. We assessed drug resistance in the dominant Gram-negative bacteria and studied the drug resistance profiles of multidrug-resistant bacteria by classifying Enterobacteriaceae and nonfermentative bacteria. We then retrospectively analyzed the clinical data and risk factors associated with 72 strains of Gram-negative bacilli, which were divided into multidrug-resistant bacteria (50 cases) and non-multidrug-resistant bacteria (22 cases). Results The main bacteria were Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa. Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli had a 66.67% detection rate. Acinetobacter baumannii had more than 50.00% drug resistance to carbapenems, ESBL-producing Klebsiella pneumoniae had 100.00% drug resistance, and Pseudomonas aeruginosa had 66.67% resistance to carbapenems. Multivariate logistic regression analysis suggested that the administration of third- or fourth-generation cephalosporins was an independent risk factor for Gram-negative multidrug-resistant biliary bacterial infection in acute pancreatitis patients. Conclusion Drug resistance among biliary pathogens in acute pancreatitis patients remains high; therefore, rational antimicrobial drug use and control measures should be carried out considering associated risk factors to improve diagnosis and treatment quality in acute pancreatitis patients.

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Section: Discussionmentioning

confidence: 99%

Characteristics of and risk factors for biliary pathogen infection in patients with acute pancreatitis

et al. 2021

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“…Fastidious and slowly growing microorganisms or broad-spectrum antimicrobial treatment before obtaining pleural/peritoneal fluids for culture often leads to false-negative results. Moreover, bacterial culture usually takes 1 to 5 or more days (She et al 2018 ). For highly suspected patients with infections, the shorter the diagnostic procedure, the better the clinical outcome may be.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR

Zhou

Sun

et al. 2020

Folia Microbiol

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Pleural and peritoneal infections cause substantial morbidity and mortality. Traditional diagnostic methods rely on the cultivation of clinical samples, which usually takes days to obtain report and holds a low detection sensitivity. In this study, we evaluated a 5-fluorescent-channel droplet digital PCR (ddPCR) system and 5 assay panels for culture-independent rapid pathogen detections directly from pleural and peritoneal fluid samples. Traditional culture of the same sample was used as reference. A total of 40 pleural fluid samples and 19 peritoneal fluid samples were tested in this study. Twenty-five positives including 4 polymicrobial infections by culture and 26 positives including 11 polymicrobial infections by ddPCR were detected for pleural fluid samples; 14 positives including 2 polymicrobial infections by culture and 15 positives including 3 polymicrobial infections by ddPCR were detected for peritoneal fluid samples. Klebsiella pneumoniae was the most common bacterium detected both in pleural and in peritoneal fluid samples. The sensitivity of the ddPCR assay for pleural and peritoneal fluid samples was 96% (95% confidence interval (CI) = 79.65 to 99.90%) and 92.86% (95% CI = 66.13 to 99.82%), respectively. The turnaround time of the ddPCR assay was approximately 3 h comparing with 38.30 ± 22.44 h for culture-based identifications. Our results demonstrated that the ddPCR assay is a rapid and sensitive method for identifying pathogens responsible for pleural and peritoneal infections and would be a promising approach for early diagnosis and optimizing treatment of infections.

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“…They reported that different blood culture systems gave close results to each other. Duration time of culture positivity was shorter and isolation rate was higher than the standard culture (16).…”

Section: Discussionmentioning

confidence: 85%

Impact of Using Automated Blood Culture System on the Isolation Success of Causative Agents of Parapneumonic Effusions

Batum¹,

Şenol²,

Çoşkun³

et al. 2021

hamidiyemedj

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Background: Complicated parapneumonic pleural effusion causes high morbidity and mortality. Identification of the etiological agent is the key element for appropriate treatment. The aim of this study is to investigate whether getting the higher isolation rate of causative bacterial agent is possible by additional bedside blood culture method for parapneumonic effusion samples.Materials and Methods: Parapneumonic effusion samples taken from patients with pneumonia between January 2015 and January 2018 were studied in the referral hospital for thoracic diseases in Turkey. Samples were processed by both standard and bedside BacT/Alert blood culture method. Isolation and identification of bacterial agents were done by standard microbiological methods. The descriptive statistics were applied.Results: Fifty one patients with pneumonia accompanied by parapneumonic effusion, who met the inclusion criteria, were included in the study. Bacterial agents were isolated by standard microbiological method in 3 (5.9%) patients and in 11 (21.5%) patients by blood culture bottle at the bedside method. Conclusion:The bedside blood culture bottle method has been found more sensitive than the standard culture method for the detection of bacterial pathogens in parapneumonic effusions.

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Performance of the BacT/Alert Virtuo Microbial Detection System for the culture of sterile body fluids: prospective multicentre study

Abstract: The BacT/Alert Virtuo system was equivalent to the 3D system in organism recovery from sterile body fluid culture but showed faster detection of growth as a result of design enhancements.

Cited by 16 publications

References 21 publications

Characteristics of and risk factors for biliary pathogen infection in patients with acute pancreatitis

Characteristics of and risk factors for biliary pathogen infection in patients with acute pancreatitis

Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR

Impact of Using Automated Blood Culture System on the Isolation Success of Causative Agents of Parapneumonic Effusions

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