Performance of the BioFire Blood Culture Identification 2 panel for the diagnosis of bloodstream infections

Peri, Anna Maria; Bauer, Michelle J; Bergh, Haakon; Butkiewicz, Dominika; Paterson, David L.; Harris, Patrick N A

doi:10.1016/j.heliyon.2022.e09983

Cited by 18 publications

(10 citation statements)

References 28 publications

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“…We observed no false‐positive BCID2 results. The high analytic sensitivity and specificity are consistent with the high concordance between BCID2 and culture‐based diagnostics in previous studies [8, 9]. A systematic review on the performance of the BCID2 panel found only few false‐positives in the 10 studies included, mainly S. epidermidis, without further specification [10].…”

Section: Discussionsupporting

confidence: 80%

“…The importance of timely and effective antimicrobial therapy in BSI is well documented [18–20]. Several studies have calculated the time gain of BCID2 compared to culture‐based diagnostics, with mean gain ranging from 9.7 h to at least 1 day [9, 21]. In our study, the use of BCID2 provided genus and/or species identification on average nearly 2 days earlier than the first preliminary culture‐based results.…”

Section: Discussionmentioning

confidence: 69%

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FilmArray (BCID2) provides essential and timely results in bloodstream infections in small acute care hospitals without conventional microbiology services

Harboe‐Sjåvik,

Endresen,

Åsheim

et al. 2024

APMIS

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We have evaluated the performance of FilmArray BCID2 in reactive blood cultures in a small acute care hospital compared to conventional diagnostics at a regional microbiological laboratory. This is a retrospective observational study of BactAlert reactive blood cultures (n = 160) from Helgeland Hospital, July–December 2021, analysed by BCID2 locally and conventional culture at a regional laboratory. The overall clinical and analytic sensitivity with BCID2 were 87.2% and 97.8%, respectively. The false‐negative BCID2 rate was low (n = 4; 2.9%). No false‐positive BCID2 results were observed. The BCID2 data were available on average 1.88 days earlier than culture‐based results, due to long transport time to the regional laboratory. The BCID2 provided results to support a significantly earlier optimized targeted antibiotic treatment in 27% of the cases according to national guidelines for empirical treatment of BSI. The high clinical and analytical sensitivity, and specificity support the use of BCID2 as a robust supplement to traditional cultivation of positive blood cultures. The significant time gain to microbial identification and detection of resistance determinants suggests a great clinical importance of BCID2 in small acute care hospitals with long transport time to conventional clinical microbiology services.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 69%

FilmArray (BCID2) provides essential and timely results in bloodstream infections in small acute care hospitals without conventional microbiology services

Harboe‐Sjåvik,

Endresen,

Åsheim

et al. 2024

APMIS

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“…In 4 out of 60 cases, BCID2 did not detect any target and these all grew BCID2 off-panel bacteria. 12 …”

Section: Discussionmentioning

confidence: 99%

Clinical Utility of Blood Culture Identification 2 Panel in Flagged Blood Culture Samples from the Intensive Care Unit of a Tertiary Care Hospital

Gopalakrishnan,

Sethuraman,

Nambi

et al. 2024

Indian Journal of Critical Care Medicine

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Background The availability of rapid diagnostic platforms for positive blood cultures has accelerated the speed at which the clinical microbiology laboratory can identify the causative organism and facilitate early appropriate antimicrobial therapy. There is a paucity of data regarding the clinical utility of the blood culture identification 2 (BCID2) panel test and its correlation with phenotypic drug susceptibility testing (DST) in flagged blood culture bottles from intensive care units (ICUs) in countries such as India, which have high rates of multidrug-resistant gram-negative bacteria (MDR-GNB). Materials and methods We conducted a retrospective observational study in a tertiary care ICU on 200 patients above 18 years of age in whom a BCID2 test was ordered when blood cultures flagged positive. Results We found 99% concordance between BCID2 and cultures in the identification of bacteria and yeasts and 96.5% concordance between phenotypic and genotypic DST. Furthermore, BCID2 was available about 1.5 days earlier than conventional ID and DST and played a key role in tailoring antimicrobials in 82.5% of the patients. Polymyxin-based therapy was discontinued earlier after an empiric dose in 138 patients (69%) based on BCID2 reports. Conclusion In critically ill patients with monomicrobial bacteremia, BCID2 rapidly identifies bacteria and antimicrobial resistance (AMR) genes and is significantly faster than conventional culture and sensitivity testing. Antibiotics were escalated in more than a third of patients and de-escalated in almost a fifth on the same day. We recommend that all ICUs routinely incorporate the test in their antibiotic decision-making process and in antimicrobial stewardship. How to cite this article Vineeth VK, Nambi PS, Gopalakrishnan R, Sethuraman N, Ramanathan Y, Chandran C, et al. Clinical Utility of Blood Culture Identification 2 Panel in Flagged Blood Culture Samples from the Intensive Care Unit of a Tertiary Care Hospital. Indian J Crit Care Med 2024;28(5):461–466.

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“…The concordance rate was significantly high at 98.1% (102/104) for the on-panel microbial species targets, which is consistent with previous studies. 15 , 20 21 22 23 24 25 The performance of BCID2 was excellent, with 100% sensitivity and 100% specificity achieved for most targets included in the panel. In the case of C. tropicalis , we concluded that these additional Candida detections were false-positives based on further investigations involving the absence of fungal nucleic acids (data not shown) and failure to recover the isolates after repeat subculture.…”

mentioning

confidence: 99%

Rapid Direct Identification of Microbial Pathogens and Antimicrobial Resistance Genes in Positive Blood Cultures Using a Fully Automated Multiplex PCR Assay

Kim,

Yun,

Cho

et al. 2024

J Korean Med Sci

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This study assessed the performance of the BioFire Blood Culture Identification 2 (BCID2) panel in identifying microorganisms and antimicrobial resistance (AMR) profiles in positive blood cultures (BCs) and its influence on turnaround time (TAT) compared with conventional culture methods. We obtained 117 positive BCs, of these, 102 (87.2%) were correctly identified using BCID2. The discordance was due to off-panel pathogens detected by culture (n = 13), and additional pathogens identified by BCID2 (n = 2). On-panel pathogen concordance between the conventional culture and BCID2 methods was 98.1% (102/104). The conventional method detected 19 carbapenemase-producing organisms, 14 extended-spectrum beta-lactamase-producing Enterobacterales, 18 methicillin-resistant Staphylococcus spp., and four vancomycin-resistant Enterococcus faecium . BCID2 correctly predicted 53 (96.4%) of 55 phenotypic resistance patterns by detecting AMR genes. The TAT for BCID2 was significantly lower than that for the conventional method. BCID2 rapidly identifies pathogens and AMR genes in positive BCs.

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Performance of the BioFire Blood Culture Identification 2 panel for the diagnosis of bloodstream infections

Cited by 18 publications

References 28 publications

FilmArray (BCID2) provides essential and timely results in bloodstream infections in small acute care hospitals without conventional microbiology services

FilmArray (BCID2) provides essential and timely results in bloodstream infections in small acute care hospitals without conventional microbiology services

Clinical Utility of Blood Culture Identification 2 Panel in Flagged Blood Culture Samples from the Intensive Care Unit of a Tertiary Care Hospital

Rapid Direct Identification of Microbial Pathogens and Antimicrobial Resistance Genes in Positive Blood Cultures Using a Fully Automated Multiplex PCR Assay

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