Performance of the Sysmex UF-1000i urine analyser in the rapid diagnosis of urinary tract infections in hospitalized patients

Le, Zhian; Li, Fengying; Fei, Chunrong; Ye, Aiqing; Xie, Xinyou; Zhang, Jun

doi:10.1016/j.jiac.2016.02.009

Cited by 18 publications

(15 citation statements)

References 14 publications

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“…Recent studies endorse its diagnostic capability for detecting UTIs (Manoni et al 2009;Pieretti et al 2010;de Rosa et al 2010;Van Der Zwet et al 2010;Broeren et al 2011;Marschal et al 2012;Boonen et al 2013;Mart ın-Guti errez et al 2015). However, the excess of false negative (FN) reported entails unclear optimum cut-offs on particle counts and, therefore, hampering its widespread implementation (Wang et al 2010;Broeren et al 2011;Muñoz-Algarra et al 2013;de Frutos-Serna et al 2014;Le et al 2016;March-Rossell o et al 2016).…”

Section: Resultsmentioning

confidence: 99%

“…; Monsen and Rydén ; Le et al . ). However, controversy about cytometer results can be drawn from medical literature (Jolkkonen et al .…”

Section: Introductionmentioning

confidence: 97%

“…These include urine strip testseither with or without microscopic examination of sedimentand Gram staining (Van Der Zwet et al 2010;Jiang et al 2011;Giesen et al 2013;St€ urenburg et al 2014). Fluorescent flow cytometry performance has spread last years (de Rosa et al 2010;Broeren et al 2011;Muñoz-Algarra et al 2013;Monsen and Ryd en 2015;Le et al 2016). However, controversy about cytometer results can be drawn from medical literature (Jolkkonen et al 2010;de Rosa et al 2010;Kadkhoda et al 2011;Muñoz-Algarra et al 2013;de Frutos-Serna et al 2014).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sysmex UF-1000i flow cytometer to screen urinary tract infections: the URISCAM multicentre study

Herráez

Asencio

Carranza

et al. 2018

Lett Appl Microbiol

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Fluorescent flow cytometry performance has recently spread for urine screening. However, controversy about cytometer results can be drawn from medical literature. This study shows the diagnosis accuracy of Sysmex UF-1000i analyzer by means of a group of decision rules encompassing both demographic variables (age) and cytometer parameters (bacteria, leucocytes and bacteria morphology). After applying the predictive algorithm, the UF-1000i could optimally identify 95% urinary tract infections with high negative predictive value and low diagnostic error. Implementation of UF-1000i would avoid culturing almost 38% of urine samples, thus reducing time to diagnosis, unnecessary antibiotic treatments and consequently improving cost-effectiveness.

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Section: Resultsmentioning

confidence: 99%

“…; Monsen and Rydén ; Le et al . ). However, controversy about cytometer results can be drawn from medical literature (Jolkkonen et al .…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sysmex UF-1000i flow cytometer to screen urinary tract infections: the URISCAM multicentre study

Herráez

Asencio

Carranza

et al. 2018

Lett Appl Microbiol

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“…The death rate was significantly higher among candiduria patients than among those without candiduria (13 [17]. Several studies [11][12][13] Manuscript to be reviewed respectively, using a cutoff value of 84.6 YLCs/μL. According to a report from Gutiérrez-Fernández J et al [11], the sensitivity, specificity, PPV and NPV of urine YLC counts for determining candiduria were 87.3%, 97.0%, 9.3% and 99.9%, respectively, with a cutoff value of ≥ 50 YLCs/μL.…”

Section: Risk Factors and Outcome Of Icu Candiduria Patientsmentioning

confidence: 99%

“…By utilizing fluorescent staining and flow cytometric technology, the Sysmex UF-1000i system measures each detected formed element, including fungi in urine, simply and efficiently. Several studies [11][12][13] have supported the reliability of an automatic urine analysis system for bacteria and yeast-like cell (YLC) detection.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #3 of "Candiduria in hospitalized patients: an investigation with the Sysmex UF-1000i urine analyzer (v0.1)"

Lee¹

2019

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Background. Candiduria is common in hospitalized patients. Its management is limited because of inadequate understanding. Previous epidemiological studies based on culture assay have been limited to small study populations. Therefore, data collected by automated systems from a large target population are necessary for more comprehensive understanding of candiduria in hospitalized patients. Methods. To determine the performance of the Sysmex UF-1000i in detecting candiduria, a cross-sectional study was designed and conducted. A total of 203 yeast-like cell (YLC)-positive and 127 negative samples were randomly chosen and subjected to microbiologic analysis. The receiver operating characteristic curve (ROC) was used to evaluate the ability of YLC counts as measured by the Sysmex UF1000i to predict candiduria. Urinalysis data from 31,648 hospitalized patients were retrospectively investigated, and statistical analysis was applied to the data collected. Results. Using a cutoff value of 84.6 YLCs/μL, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the yeast like cell (YLC) counts to predict candiduria were 61.7%, 84.1%, 88.6% and 66.3%, respectively. C. glabrata (33.6%) and C. tropicalis (31.4%) were more prevalent than C. albicans (24.3%) in the present study. Of the investigated hospitalized patients, 509 (1.61%) were considered candiduria-positive. Age, gender and basic condition were associated with candiduria in hospitalized patients. In the ICU setting, urinary catheterization appeared to be the only independent risk factor contributing to candiduria according to our investigation. Although antibiotic therapy has been reported to be a very important risk factor, we could not confirm its significance in ICU candiduria patients because of excessive antibiotic usage in our hospital. Conclusions. The YLC measured by Sysmex UF-1000i is a practical and convenient tool for clinical candiduria screening prior to microbiologic culture. Candiduria is common in hospitalized patients, and its incidence varies according to age, gender and the wards where it is isolated. Candiduria had no direct connection with mortality but might be considered a marker of seriously ill patients who need particular attention in the clinic.

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High false positive rate of white blood cells in urine samples of pregnant women may be caused by epithelial cells being misclassified by the sysmex UF‐1000i urine flow cytometer

Yang

Liú

et al. 2018

Cytometry Part B Clinical

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BackgroundThe UF‐1000i has been widely used in screening urinary sediments. However, the interference factor of the UF‐1000i in the screening urinary sediments of pregnant women has not been reported. The aim of the study was to demonstrate that epithelial cells (ECs) cause a high false positive rate of white blood cells (WBCs) by the UF‐1000i in pregnant women.MethodsUrine samples were collected from 207 pregnant women. All samples were measured by the UF‐1000i and a microscopic method.ResultsThe areas under the curve (AUC) for WBC and EC counts were 0.837 (95% CI, 0.773–0.901) and 0.844 (95% CI, 0.785–0.903), respectively. The positive rates of the WBC and EC were 73.43% and 37.20%, respectively, by the UF‐1000i, and they were 19.32% and 72.95% by the microscopic method. The positive predictive value, negative predictive value, false positive rates, and false negative rates by the UF‐1000i were for WBC 25.66%, 98.18%, 74.34%, and 1.82%, respectively, and for EC they were 96.1%, 40.77%, 3.9%, and 59.23%, respectively. The coefficient of correlation R value was 0.503 (P < 0.01) between WBC by UF‐1000i and EC by the microscopic method in WBC false positive samples.ConclusionsEC could be an interference factor for the UF‐1000i in screening urinary WBC of pregnant women, and the high false positive rate for WBC may be caused by ECs being misclassified as WBCs by the UF‐1000i. © 2018 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.

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Performance of the Sysmex UF-1000i urine analyser in the rapid diagnosis of urinary tract infections in hospitalized patients

Cited by 18 publications

References 14 publications

Sysmex UF-1000i flow cytometer to screen urinary tract infections: the URISCAM multicentre study

Sysmex UF-1000i flow cytometer to screen urinary tract infections: the URISCAM multicentre study

Peer Review #3 of "Candiduria in hospitalized patients: an investigation with the Sysmex UF-1000i urine analyzer (v0.1)"

High false positive rate of white blood cells in urine samples of pregnant women may be caused by epithelial cells being misclassified by the sysmex UF‐1000i urine flow cytometer

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