Solar thermal energy storage improves the practicality and efficiency of solar systems for space heating by addressing the intermittent nature of solar radiation, leading to enhanced energy utilization, cost reduction, and a more sustainable and environmentally friendly approach to meeting heating needs in residential, commercial, and industrial settings. In this study, an indoor experimental setup was employed to investigate the impact of a water-based Al2O3 nanofluid on the storage capacity of a flat plate solar collector under varying flow rates of the heat transfer fluid. The nanofluid, introduced at specific concentrations, was incorporated into a water-contained storage tank through which the hot heat transfer fluid circulated within a heat exchanger. This process resulted in the storage of thermal energy for future applications. The research identified that the optimal flow rate of the heat transfer fluid, corresponding to the maximum storage temperature, was 15 L per hour, and the ideal nanofluid concentration, associated with the maximum specific heat capacity of the storage medium, was 0.6%. Furthermore, the introduction of nanoparticles into the storage tank led to a significant increase in the specific heat of the water, reaching a maximum of 19% from 4.18 to 5.65 kJ/(kg·°C).