Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor

Hanafiah, Zarimah Mohd; Mohtar, Wan Hanna Melini Wan; Hasan, Hassimi Abu; Jensen, Henriette Stokbro; Klaus, Anita; Wan‐Mohtar, Wan Abd Al Qadr Imad

doi:10.1038/s41598-019-52493-y

Cited by 30 publications

(9 citation statements)

References 43 publications

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“…According to the study of Hanafiah et al, the microstructures of PAPS and P. alhagi NX-11 treated under different pH conditions were plotted as shown in Figure 8 and Figure 9 [ 57 ]. Based on the results of previous experiments, we selected several representative samples for analysis (pH 2, pH 7, pH 9, pH 12, and pH 13).…”

Section: Resultsmentioning

confidence: 99%

High-Efficiency Extraction of Pantoea alhagi Exopolysaccharides Driven by pH-Related Changes in the Envelope Structure

Sun

Wang

et al. 2022

Molecules

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Increasing numbers of exopolysaccharides and their properties have been explored. However, the difficulty of extracting high-viscosity exopolysaccharides has hindered their further industrialization. In this research, we explored a strategy based on encapsulated structure control under different pH to efficiently extract Pantoea alhagi exopolysaccharides (PAPS). Results showed that at pH levels of 6, 12, and 13, the extraction efficiency of PAPS was elevated, and the yield did not decrease. The rheological properties of the pH−12-treated PAPS were better than those of PAPS treated at pH 7, while the pH−6-treated PAPS decreased. The effects of pH−12-treated PAPS on soil macroaggregates and soil’s water evaporation rate were similar to those of PAPS treated at pH 7. In addition, we observed that treatment at pH 12 produced a significantly reduced encapsulated structure compared with treatment at pH 7. The proportion of unsaturated fatty acids after treatment at pH 12 was higher than after treatment at pH 7, which may result in reduced encapsulated structure in pH−12 conditions. These results enrich the understanding of the effect that alters pH conditions on the encapsulated structure to improve the extraction efficiency of exopolysaccharides and provide a theoretical basis for the extraction of exopolysaccharides with extreme viscosity.

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Section: Resultsmentioning

confidence: 99%

High-Efficiency Extraction of Pantoea alhagi Exopolysaccharides Driven by pH-Related Changes in the Envelope Structure

Sun

Wang

et al. 2022

Molecules

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“…Table 2 compares the costs of different culture media used for G. lucidum submerged culture, with relation to the one used in this work-study. For the culture media reported in the literature, preparation costs range from 4 to 16 USD/L [31][32][33][34], being carbon and nitrogen sources responsible for more than 70% of the total culture medium cost. The proposed medium based on barley flour has a total preparation cost of 0.14 USD/L, which represents a saving of more than 97% in raw material.…”

Section: Media Composition and Biomass Productionmentioning

confidence: 99%

Volumetric Oxygen Transfer Coefficient Effect on Biomass, Bioactive Compounds Production, and Kinetic Behavior of G. lucidum in Submerged Culture Using a Complex Medium.

Romo-Buchelly

Sepúlveda-Arango

Restrepo-Restrepo

et al. 2022

Braz. arch. biol. technol.

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The effect of volumetric oxygen mass transfer coefficient (KLa) on the biomass and the commercial interest biomolecules production, was studied in the Ganoderma lucidum submerged culture using a lowcost complex medium. The batch culture was carried out in a 14 L stirred tank bioreactor, a KLa initial value set at 5.3 h -1 had a positive effect on exopolysaccharides production, achieving a maximum concentration of 1.15 g/L, under this condition, biomass production was negatively affected. An increase in the KLa initial values between 17.3 -32.8 h -1 , allowed us to obtain a culture with better oxygen and nutrients transfer conditions. These values improved the kinetic carbohydrate uptake, fungal growth, and enabled the adequate supply of oxygen for a long time, achieving a maximum biomass concentration of 9.8 g/L. Under these conditions, the average production of exopolysaccharides is 0.46 g/L, and crude protein extract production was around 0.3 g/L. The complex medium (made from barley flour) used in this study is a low-cost medium that showed great potential for the replacement of synthetic high-cost media that are conventionally used in Ganoderma lucidum submerged cultures in a stirred tank bioreactor.

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“…The isolated pure culture was Gram-stained and observed under a light microscope [27] to identify their morphological characteristics. This isolated pure culture was also observed under 1000× magnification using a Scanning Electron Microscope (SEM) (Brand ZEISS, Model MERLIN Compact, Oberkcohen, Germany) [28] to confirm its morphological structure.…”

Section: Morphological Analysismentioning

confidence: 99%

Isolation, Identification, and Optimization of γ-Aminobutyric Acid (GABA)-Producing Bacillus cereus Strain KBC from a Commercial Soy Sauce moromi in Submerged-Liquid Fermentation

et al. 2020

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A new high γ-aminobutyric acid (GABA) producing strain of Bacillus cereus was successfully isolated from soy sauce moromi. This B. cereus strain named KBC shared similar morphological characteristics (Gram-positive, rod-shaped) with the reference B. cereus. 16S rRNA sequence of B. cereus KBC was found to be 99% similar with B. cereus strain OPWW1 under phylogenetic tree analysis. B. cereus KBC cultivated in unoptimized conditions using De Man, Rogosa, Sharpe (MRS) broth was capable of producing 523.74 mg L−1 of GABA within five days of the cultivation period. By using response surface methodology (RSM), pH level, monosodium glutamate (MSG) concentration and temperature were optimized for a high concentration of GABA production. The pH level significantly influenced the GABA production by B. cereus KBC with p-value = 0.0023. GABA production by B. cereus KBC under the optimized condition of pH 7, MSG concentration of 5 g L−1 and temperature of 40 °C resulted in GABA production of 3393.02 mg L−1, which is 6.37-fold higher than under unoptimized conditions. Overall, this study has shown that B. cereus KBC isolated from soy sauce moromi is capable of producing a high concentration of GABA together with the optimal fermentation conditions that have been statistically analysed using RSM.

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Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor

Cited by 30 publications

References 43 publications

High-Efficiency Extraction of Pantoea alhagi Exopolysaccharides Driven by pH-Related Changes in the Envelope Structure

High-Efficiency Extraction of Pantoea alhagi Exopolysaccharides Driven by pH-Related Changes in the Envelope Structure

Volumetric Oxygen Transfer Coefficient Effect on Biomass, Bioactive Compounds Production, and Kinetic Behavior of G. lucidum in Submerged Culture Using a Complex Medium.

Isolation, Identification, and Optimization of γ-Aminobutyric Acid (GABA)-Producing Bacillus cereus Strain KBC from a Commercial Soy Sauce moromi in Submerged-Liquid Fermentation

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