Textile wastewater contains many pollutants which is hazardous if directly discharged. Various technologies such as adsorption, advanced oxidation processes, biological treatment, membrane technology, coagulation, and flocculation have been explored and utilized to treat textile wastewater. However, coagulation followed by flocculation is still widely used. Usually, inorganic salts such as alum, ferric chloride, PAC, are widely used to treat textile wastewater. However, utilization of inorganic salts poses some drawbacks, such as high coagulant cost, a large volume of sludge produced, and potential health problems if the water is consumed. In this study, we explore the utilization of natural coagulant as an alternative to inorganic salts to treat textile wastewater. This study describes the utilization of papaya seeds powder as a natural coagulant for synthetic textile wastewater of drimarene dark red (DDR) with an initial concentration of 10 mg/L. The effect of coagulant dosage and pH to the %removal of color in wastewater was studied using central composite design. The potential active functional groups in papaya seeds powder were characterized using Fourier Transform Infrared Spectroscopy (FTIR). It was found that papaya seeds powder contained-OH,-NH, C=O functional groups that indicate good potential as natural coagulant. The cubic model obtained was in a good fit with experimental data, which was shown in the R-squared value of 0.995. It was found that coagulant dosage, pH, and its interaction were significant to the removal of synthetic dye in wastewater. The decrease of pH gave higher %removal due to protonation of papaya seeds powder active coagulating agent resulting in better electrostatic interaction with dyes. The increase of dosage also gave an increase in %removal until its optimum condition. After optimum condition, the %removal decreased due to colloid re-stabilization. The optimum condition was obtained at a dosage of 0.57g/L, and pH 1.97 with 84.77% of predicted color removal and this result was in agreement with experimental response value.