Perfusing dentine with horse serum or physiologic saline: its effect on adhesion of dentine bonding agents

Augustin, Claudia; Sj, Paul; Lüthy, H; Schärer, P

doi:10.1046/j.1365-2842.1998.00276.x

Cited by 9 publications

(6 citation statements)

References 18 publications

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“…Serum was used to cause a precipitation with dentin bonding agents. A coagulation/precipitation of organic and inorganic constituents seemed to alter the permeability of dentin 30. In the present study, MCN or FBS used in Step 2 should have influenced the surface characteristics of resin composites differently compared to PBS control.…”

Section: Discussionmentioning

confidence: 57%

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Influence of a series of organic and chemical substances on the translucency of resin composites

2005

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The objective of this study was to investigate the changes in the translucency of resin composites following a series of immersion treatments in organic and chemical substances. Color of resin composites was measured according to the CIELAB color scale relative to the standard illuminant D65 over a white and a black background. Translucency parameter (TP) of resin composites was calculated at baseline, and after sequential immersion: Step 1, porcine liver esterase (a substitute for a salivary esterase); Step 2, organic substances (mucin and serum) and phosphate-buffered saline (PBS) as a control; Step 3, chemical alteration agents [chlorhexidine (CH) and carbamide peroxide (CP)]; and Step 4, 2% methylene blue. Porcine liver esterase caused small changes in TP (DeltaTP = -0.5 to 0.2). After Step 2, DeltaTP values of three groups were similar in the range of -0.7 to 1.2. After Step 3, DeltaTP values of all groups were small in the range of -1.5 to 2.2. After Step 4, DeltaTP values were high and differences that varied by the resin composite and the immersion protocol were clearly observed (DeltaTP = -13.4 to -2.5). Changes in TP were mainly influenced by resin composite. After Step 4, mucin and serum groups showed generally small changes in TP compared to PBS group. It is a possibility that the high changes in TP after immersion in methylene blue is an indication of dye absorption which might be an indication of the degree of resin composite degradation.

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Section: Discussionmentioning

confidence: 57%

“…A coagulation/precipitation of organic and inorganic constituents seemed to alter the permeability of dentin. 30 In the present study, MCN or FBS used in Step 2 should have influenced the surface characteristics of resin composites differently compared to PBS control. However, this difference was not reflected in TP value after Step 2.…”

Section: Discussionmentioning

confidence: 71%

Influence of a series of organic and chemical substances on the translucency of resin composites

2005

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“…A wide range of perfusing solutions, such as sterile phosphate-buffered saline, 16 physiologic saline, 20,21 horse serum, 23 bovine serum, 24 and protein solutions, 22 have been tested in different studies in attempts to reproduce the dentinal fluid in vitro. But little is known about the exact composition of this dentinal fluid, although seems to be that albumin and globulins are the main components.…”

Section: Discussionmentioning

confidence: 99%

“…But little is known about the exact composition of this dentinal fluid, although seems to be that albumin and globulins are the main components. 23 The presence of proteins in the perfusing solution may cause a precipitation within the tubules, blocking them and reducing the dentin permeability. 23 -25 This coagulation effect is not likely to occur when distilled water is used, as in this study.…”

Section: Discussionmentioning

confidence: 99%

Inverse relationship between tensile bond strength and dimensions of bonded area

2003

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It is a known fact that there is a relationship between magnitude of bonded area and laboratory tensile test results. This relationship has been described for a range of areas between 1 and 10 mm 2 , in extracted, nonperfused teeth. The aim of this study is to test this relationship in perfused teeth, with bonded areas ranging from 0.7 to 110.9 mm 2 . Dentin of 92 sound third human molars was exposed and perfused, and three groups of bonded areas (

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“…The measurement of bond strength in an in vitro simulated oral environment has become recently a popular method for researchers (Mitchem & Gronas, 1991; Prati, Pashley & Montanari, 1991a; Nikaido et al ., 1995). For this reason, many bond strength studies have been conducted under simulated pulpal pressure in laboratory conditions (Gerzina & Hume, 1995; Camps et al ., 1996; Augustin et al ., 1998). The perfusion of the teeth with a solution, which resembles the dentinal liquid, imitates clinical conditions closer.…”

Section: Introductionmentioning

confidence: 99%

The effect of simulated intrapulpal pressure on bond strength to enamel and dentine

Şengün

Öztürk

Özer

2003

J of Oral Rehabilitation

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The purpose of this study was to evaluate the effect of simulated intrapulpal pressure on the shear bond strength (SBS) of a self-etching bonding system (Clearfil Liner Bond II) to both enamel and dentine surfaces. Forty-two caries-free human molar teeth were randomly assigned to two equal groups (n=21). One group of 21 teeth was connected to a special pulpal pressure machine, which is developed to simulate in vivo conditions. The buccal and lingual surfaces of the teeth were prepared with a diamond wheel to create flat dentine and enamel surfaces. A self-etching bonding system was then applied under pulpal pressure and 2.5 mm diameter composite cylinders were bonded to the prepared surfaces. The samples were then stored in distilled water at room temperature for 24 h under pulpal pressure before SBS was tested. The second restored group was used as a control and the teeth were not connected to the pulpal pressure apparatus during material application and test procedures. They were stored for 24 h in distilled water at room temperature before the SBS test. Fracture analysis of the enamel and dentinal surfaces was performed using a stereomicroscope. Shear bond strength values to dentine was significantly reduced with pulpal pressure (P < 0.001). However, in the enamel, SBS was increased (P < 0.001). In the control group, the SBS values to dentine and enamel did not show any significant difference (P > 0.05). Based on these results, it can be concluded that further analysis of enamel histology and bonding mechanisms are needed.

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Perfusing dentine with horse serum or physiologic saline: its effect on adhesion of dentine bonding agents

Cited by 9 publications

References 18 publications

Influence of a series of organic and chemical substances on the translucency of resin composites

Influence of a series of organic and chemical substances on the translucency of resin composites

Inverse relationship between tensile bond strength and dimensions of bonded area

The effect of simulated intrapulpal pressure on bond strength to enamel and dentine

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