Perfusion Chambers

Groot, Philip G. de; Sixma, Jan J.

doi:10.1016/b978-012369367-9/50794-1

Cited by 12 publications

(11 citation statements)

References 69 publications

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“…Gray horizontal line indicates platelet response for channel without collagen coating. B. Inhibition of thrombus formation data generated using microfluidic devices at low and high shear on different coatings using a single donor (C) IC 50 dose response for Abciximab on collagen I using 0e20 dyn/ cm 2 microfluidic device at 10 dyn/cm 2 (-) IC 50 dose response for Abciximab on VWF using a 0e200 dyn/cm 2 …”

Section: Discussionmentioning

confidence: 99%

“…Controls of no coating and no Abciximab were included in each experiment. Fibrillar collagen I experiments were performed for 2 or 5 min (dose response) at 10 dyn/ cm 2 . VWF experiments were performed at 200 dyn/cm 2 for 5 min.…”

Section: Microfluidic Device Studiesmentioning

confidence: 99%

“…2,4 Common perfusion configurations include parallel plate-flow chambers (PPFCs) or glass capillary tube-flow chambers. Typically, the flow is driven by a syringe or peristaltic pump placed alongside the chamber to generate wall shear stress that is similar to blood flow in the vasculature.…”

Section: Introductionmentioning

confidence: 99%

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Using Well-Plate Microfluidic Devices to Conduct Shear-Based Thrombosis Assays

Conant

Nevill

Zhou

et al. 2011

JALA: Journal of the Association for Laboratory Automation

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S hear stress plays a critical role in regulating platelet adhesion and thrombus formation at the site of vascular injury. As such, platelets are often examined in vitro under controlled shear flow conditions for their hemostatic and thrombotic functions. Common shear-based platelet analyses include the evaluation of genetic mutants, inhibitory or experimental compounds, matrix substrates, and the effects of different physiological and pathological shear forces. There are several laboratory instruments widely used for studying shear flow, including cone and plate viscometers and parallel plate perfusion chambers. These technologies vary widely in the types of samples, substrates, blood volumes, and throughput that are involved. Here, we describe a microfluidic system for platelet analysis under shear flow. We used the devices to study thrombus formation on collagen I and von Willebrand factor. The system was also used to investigate dose response to the antiplatelet compound, Abciximab, under shear flow conditions with an emphasis on maximizing the number of data points per single patient sample. The presented method confers multiple advantages over conventional approaches. These include the ability to assess up to 24 conditions simultaneously in real time, maintain identical physical conditions across experiments, and use extremely low donor volumes. ( JALA 2011;16:148-52)

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Section: Discussionmentioning

confidence: 99%

Section: Microfluidic Device Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Using Well-Plate Microfluidic Devices to Conduct Shear-Based Thrombosis Assays

Conant

Nevill

Zhou

et al. 2011

JALA: Journal of the Association for Laboratory Automation

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“…Perfusion chambers with matrix proteins or complete ECM have been introduced in the 1970s [93][94][95]. Under flow conditions adherence of platelets is affected by rheological conditions such as shear rate, presence of red blood cells, red blood cell deformability, and viscosity of the medium [96,97]. Later perfusion chambers were adapted to smaller volumes of blood [98].…”

Section: Platelet Adhesion and Spreadingmentioning

confidence: 99%

State of the Art in Platelet Function Testing

Kehrel

Brodde

2013

Transfus Med Hemother

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Platelets perform many functions in hemostasis but also in other areas of physiology and pathology. Therefore, it is obvious that many different function tests have been developed, each one conceived and standardized for a special purpose. This review will summarize the different fields in which platelet function testing is currently in use; diagnostics of patients with bleeding disorders, monitoring patients' response to anti-platelet therapy, monitoring in transfusion medicine (blood donors, platelet concentrates, and after transfusion), and monitoring in perioperative medicine to predict bleeding tendency. The second part of the review outlines different methods for platelet function testing, spanning bleeding time, and platelet counting as well as determining platelet adhesion, platelet secretion, platelet aggregation, platelet morphology, platelet signal transduction, platelet procoagulant activity, platelet apoptosis, platelet proteomics, and molecular biology.

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“…Shear loading within blood vessels in healthy individuals varies from 0.1 to 20 dyn/cm 2 and affects many biological phenomena from biochemistry to gene expression. 3 Thus, cell adhesion and transmigration are often studied within parallel plate flow chambers to mimic the fluid dynamics found within the circulatory system.…”

mentioning

confidence: 99%