Perfusion immunoassay for acetylcholinesterase: analyte detection based on intrinsic activity

Vanderlaan, Martin; Lotti, Robert; Siek, Gordon; King, D. T.; Goldstein, M. M.

doi:10.1016/0021-9673(95)00059-v

Cited by 14 publications

(9 citation statements)

References 11 publications

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“…Depending on the desired level of detection, UV/visible absorbance, fluorescence and MS have all been used for detecting analytes in the on/off mode of IAC. Specific examples of analytes that have been measured by this approach include human serum albumin [59,60], recombinant tissue-type plasminogen activator [61], recombinant antithrombin III [61], IgG [59,62], Escherichia coli [63], isoproturon [64], phenylurea herbicides [64,65], benzidine [66], dichlorobenzidine [66], aminoazobenzene [66], azo dyes [66], triazine [65], diethylstilbestrol [67], acetylcholinesterase [68], transferrin [69] and insulin [70]. …”

Section: Formats and Applications Of Iacmentioning

confidence: 99%

Immunoaffinity Chromatography: An Introduction to Applications and Recent Developments

2010

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Immunoaffinity chromatography (IAC) combines the use of LC with the specific binding of antibodies or related agents. The resulting method can be used in assays for a particular target or for purification and concentration of analytes prior to further examination by another technique. This review discusses the history and principles of IAC and the various formats that can be used with this method. An overview is given of the general properties of antibodies and of antibody-production methods. The supports and immobilization methods used with antibodies in IAC and the selection of application and elution conditions for IAC are also discussed. Several applications of IAC are considered, including its use in purification, immunodepletion, direct sample analysis, chromatographic immunoassays and combined analysis methods. Recent developments include the use of IAC with CE or MS, ultrafast immunoextraction methods and the use of immunoaffinity columns in microanalytical systems.

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Section: Formats and Applications Of Iacmentioning

confidence: 99%

Immunoaffinity Chromatography: An Introduction to Applications and Recent Developments

2010

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“…Irth et al presented different strategies to accomplish immunochemical interactions in on-line post-column detection techniques (Irth et al, 1995). Either labeled antibodies (Irth et al, 1993;Oosterkamp et al, 1994aOosterkamp et al, 1998Irth et al, 1995;Miller and Herman, 1996;Cho et al, 1996) or labeled antigens (Oosterkamp et al, 1994b;Irth et al, 1995;Vanderlaan et al, 1995;Oosterkamp et al, 1996aOosterkamp et al, , 1996bOosterkamp et al, , 1997aOosterkamp et al, , 1997bCho et al, 1996) can be used to detect analytes eluting from the HPLC column. Bound and unbound labeled antibodies or labeled antigens are separated prior to detection.…”

Section: Post-column Immunoassay Coupled To Lcmentioning

confidence: 99%

“…Other formats of immunoassay used in the postcolumn mode include direct detection immunoassays (Vanderlaan et al, 1995;Cho et al, 1996), one-site immunoassays (Irth et al, 1993(Irth et al, , 1995Oosterkamp et al, 1994aOosterkamp et al, , 1997aOosterkamp et al, , 1998Miller and Herman, 1996), sandwich immunoassays (Cho et al, 1996) and competitive binding immunoassays (Oosterkamp et al, 1994b(Oosterkamp et al, , 1996a(Oosterkamp et al, , 1996b(Oosterkamp et al, , 1997a(Oosterkamp et al, , 1997bIrth et al, 1995;Shahdeo and Karnes, 1999;Shahdeo et al, 2000;Graefe et al, 2000).…”

Section: Post-column Immunoassay Coupled To Lcmentioning

confidence: 99%

“…GHRF was measured using an UV detector at a wavelength of 280 nm. A second example of the direct mode in post-column immunodetection was provided by Vanderlaan et al (1995). In their work, amniotic fluid proteins containing acetylcholinesterases (AChE) were first fractionated on a size-exclusion column, the fraction containing neural AChE was then diverted to a second column, an immobilized immunoaffinity column with anti-AChE antibodies.…”

Section: Post-column Immunoassay Coupled To Lcmentioning

confidence: 99%

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Coupling immunoassays with chromatographic separation techniques

Tang

Karnes

2000

Biomed. Chromatogr.

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Coupling immunoassays with HPLC separation techniques is becoming increasingly useful in the analysis of biological and nonbiological samples of both large and small molecules. This is because it provides both sensitivity and selectivity for molecular analysis at relatively low cost, low maintenance and with excellent potential for automation. This paper reviews application of this hyphenated approach both in the pre-column immunoextraction and post-column immunodetection modes. Systems in which immunoassays are interfaced to chromatographic separations in order to separate bound and free fractions of the immunoassay will not be included since these systems do not provide the enhanced selectivity common to hyphenated systems. Post-column immunodetection is based on various immunoassay formats such as direct detection, one-site, competitive and sandwich immunoassays. Homogeneous immunodetectors are more convenient than heterogeneous immunodectors since there are no separation and column regeneration steps involved in homogeneous immunoassays. On the other hand, heterogeneous immunoassays are generally more sensitive than homogeneous immunoassays since interfering substances are removed prior to immunodetection. Advantages and limitations for the various approaches will be discussed.

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“…Fluorescein-labeled F ab fragments (raised against digoxigenin) and immobilized digoxin were used in this postcolumn detection system. Related methods based on direct detection or sandwich immunoassays have been used to monitor acetylcholinesterase and bovine growth hormone releasing factor (36,37). A competitive binding immunoassay has not formally been used for postcolumn detection, but such an approach has been demonstrated in principle with other affinity systems, such as the binding of avidin to biotin (35).…”

Section: P Po Os St Tc Co Ol Lu Um Mn N I Im Mm Mu Un No Od De Et Te mentioning

confidence: 99%

Peer Reviewed: Chromatographic Immunoassays

Hage¹,

Nelson²

2001

Anal. Chem.

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Perfusion immunoassay for acetylcholinesterase: analyte detection based on intrinsic activity

Cited by 14 publications

References 11 publications

Immunoaffinity Chromatography: An Introduction to Applications and Recent Developments

Immunoaffinity Chromatography: An Introduction to Applications and Recent Developments

Coupling immunoassays with chromatographic separation techniques

Peer Reviewed: Chromatographic Immunoassays

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