Perfusion of Gat Kidneys from Heart-Lung Circuits.

Abstract

7and the larger spleens cannot be satisfactorily explained at present.Summary. Liver catalase activity in Friend virus-infected germfree mice is significantly depressed below normal values at a stage when splenomegaly is marked and liver involvement slight. The indications are that splenomegaly alone is related to lo'wered liver catalase rather than mild liver damage or the presence of virus as such. The existence of a spleen-elaborated catalase inhibitory factor is postulated. The value of germfree mice 'in this study lay in the avoidance of the possibility of bacterial products affecting the liver catalase activity.

The influence of the polar anastomotic vessels on autoregulation and the urinary excretion of water and sodium by perfused cat kidneys

“…Kidneys were prepared for perfusion as described by Lockett (1966). The two ventral groups of polar anastomotic vessels in the kidneys of young cats, together with their dorsal counterparts, are often the sole visible vessels leaving the capsule to anastomose with other vessels in the perirenal fat.…”

“…Sixteen heart-lung kidney preparations were made as previously described (Lockett, 1959, 1966, Davey & Lockett 1960 in cats weighing 0-8-1-5 kg. Twenty kidneys, taken from cats weighing 0-7-1-8 kg, were perfused from pump-oxygenator circuits.…”

“…The oxygenator and the valve chambers stood in a tank of water maintained at 390 C and stirred by a combined thermostat and pump unit (Grant Instrument Co. Ltd.) which also perfused the warming jacket of the kidney chamber. Measurements of arterial pressure, haematocrit, renal blood and urine flows, clearances of creatinine and PAH and of concentrations of sodium and potassium in plasma and urine were made as described previously (Lockett, 1966 Pressure change was always effected by adjustment of the peripheral resistance. The PC02, pH and P02 of blood were measured on 0 5 ml.…”

“…By contrast, cat kidneys perfused from heartlung circuits retain considerable ability to concentrate urine but show gross impairment of their autoregulative capacity (Lockett, 1966).…”

Effects of salt loading and haemodilution on the responses of perfused cat kidneys to angiotensin

“…Heart-lung-kidney preparations were made as previously described (Lockett, 1959;1966a;Davey & Lockett, 1960) in cats weighing 1 2-2-1 kg. Anaesthesia was induced with ether and was maintained by the i.v.…”