Perilipin and adipophilin expression regulated by Rho‐kinase during differentiation of hamster sebocyte

Kaneta, Kazuhiro; Honma, Masaru; Kishibe, Mari; Iizuka, Hajime

doi:10.1111/j.1346-8138.2011.01359.x

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Based on these results, PLIN3 seems to play a role in the synthesis of TGs with high unsaturation degree, suggesting that PLIN3 influences specific pathways governing the transfer of acyl moieties to the receiving glycerol backbone. Moreover, the array of TG species affected by PLIN3 differed from those influenced by PLIN2 expression (17).…”

Section: Resultsmentioning

confidence: 99%

“…Although lipid accumulation is the hallmark of sebocyte differentiation, the composition and regulation of LD-associated proteins in these cells have been poorly characterized (16,17). We showed previously that PLIN2 expression is regulated during sebocyte differentiation and is important for lipid accumulation in vitro and for regulating SG size in vivo (18).…”

Section: Introductionmentioning

confidence: 99%

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Perilipin 3 modulates specific lipogenic pathways in SZ95 sebocytes

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Dahlhoff²,

Ludovici

et al. 2014

Experimental Dermatology

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In recent years, the scientific community has generated an ever-increasing amount of data from a growing number of animal models of human cancers. Much of these data come from genetically engineered mouse models. Identifying appropriate models for skin cancer and related relevant genetic data sets from an expanding pool of widely disseminated data can be a daunting task. The Mouse Tumor Biology Database (MTB) provides an electronic archive, search and analysis system that can be used to identify dermatological mouse models of cancer, retrieve modelspecific data and analyse these data. In this report, we detail MTB's contents and capabilities, together with instructions on how to use MTB to search for skin-related tumor models and associated data.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Perilipin 3 modulates specific lipogenic pathways in SZ95 sebocytes

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Dahlhoff²,

Ludovici

et al. 2014

Experimental Dermatology

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“…Favored examples are mammary epithelial cells, steroidogenic cells of the adrenal cortex and gonads, and macrophages [17]. Although lipid accumulation is a key feature of sebocyte differentiation, the study of the composition and regulation of LD-associated proteins in these cells has been limited to a few reports [18,19]. There are, however, many facts suggesting that LD biology may differ significantly between adipocytes and sebocytes.…”

Section: Introductionmentioning

confidence: 99%

PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo

Dahlhoff¹,

Camera

Picardo

et al. 2013

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background Lipid synthesis and storage are accomplished by lipid droplets (LDs). The perilipin family of LD-associated proteins, comprising 5 members (PLIN1-PLIN5), has been well characterized in adipocytes but not in sebocytes, epithelial cells in which LD formation is a key feature of the cellular differentiation. Methods Perilipin expression in the sebaceous gland cell line SZ95 and in human sebaceous glands was studied by qRT-PCR, Western blots, and immunohistochemistry. Lipid accumulation was evaluated by Nile red staining and mass spectrometry. Results PLIN2 and PLIN3 are the most abundant perilipins in undifferentiated sebocytes. Induction of lipogenesis by linoleic acid (LA) resulted in increased transcript levels of all perilipins except for PLIN3 and in a time-dependent increase of PLIN2 protein. Nile red staining revealed that siRNA-mediated downregulation of PLIN2 significantly impaired basal and LA-induced lipid accumulation. Mass spectrometry revealed PLIN2 deficiency to cause a reduction in the amount of several specific lipid fractions, including di- and triacyl-glycerol esters, phosphatidylcholine lipids, and ceramides in sebocytes under basal conditions. In contrast, PLIN2 downregulation exerted a statistically significant inhibitory effect only on the accumulation of specific LA-induced triglycerides. PLIN2-deficient mice showed normal morphology of sebaceous glands. However, their sebaceous glands were significantly reduced in size and showed less cell proliferation. Conclusions PLIN2 is the major perilipin regulated during sebocyte differentiation in vitro. PLIN2 is also important for sebaceous lipid accumulation in vitro and regulates sebaceous gland size in vivo. General significance Our study provides the first systematic analysis of LD-associated proteins in sebocytes.

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Lipid droplets and associated proteins in the skin: basic research and clinical perspectives

Schneider¹,

Zhang

2015

Arch Dermatol Res

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Lipid droplets (LDs), the major organelles handling fat storage, comprise a hydrophobic neutral lipid core surrounded by a phospholipid monolayer embedded with a protein miscellany. Although lipids of the stratum corneum are essential for the skin barrier, and progressive lipid accumulation culminating in cell disruption is the hallmark of sebaceous differentiation, only a few studies touched on skin LD and associated proteins so far. Here, after briefly introducing the basic facts about LD and associated proteins, we discuss how forthcoming studies may unveil novel players in skin lipid metabolism and candidate target proteins for treating skin diseases.

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Perilipin and adipophilin expression regulated by Rho‐kinase during differentiation of hamster sebocyte

Cited by 4 publications

References 9 publications

Perilipin 3 modulates specific lipogenic pathways in SZ95 sebocytes

Perilipin 3 modulates specific lipogenic pathways in SZ95 sebocytes

PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo

Lipid droplets and associated proteins in the skin: basic research and clinical perspectives

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