Perinatal concentrations of 17β-estradiol and testosterone in the toe claws of female and male dogs from birth until 60 days of age

Fusi, Jasmine; Comin, A.; Faustini, M.; Prandi, Alberto; Veronesi, Maria Cristina

doi:10.1016/j.anireprosci.2020.106313

Cited by 5 publications

(14 citation statements)

References 15 publications

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“…However, in valuable dogs, the owners often refuse to shave the hair, even in a small area, limiting the use of the coat as a matrix of study in many instances. A couple of recent studies [26,28] showed the usefulness of the claws as an alternative to the coat for the same long-term studies in newborn puppies. For these reasons, in the present study, both the coat and the claws were collected with the shave-re-shave and clip-re-clip method for the longitudinal analysis of C and DHEA-S long-term changes.…”

Section: Discussionmentioning

confidence: 99%

“…Despite most of the studies referring to concentrations of C and DHEA/DHEA-S in the blood or less invasive matrices such as urines, feces and saliva, all those matrices depict only the concentration of a given time point, and their use is therefore not suitable when long-lasting physiological processes have to be investigated because of the need to repeat multiple samplings. To overcome these issues, in the last years, new matrices, like the hair/coat and nails/claws, have been proposed for long-term hormonal studies in humans and animals [24][25][26][27][28] thanks to their characteristic of providing retrospective information about long-term hormone accumulation. Thus, the coat and claws could be suitable matrices for the study of hormonal changes occurring in the long-lasting phase between mating of the female dog to puppies weaning.…”

Section: Introductionmentioning

confidence: 99%

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How Stressful Is Maternity? Study about Cortisol and Dehydroepiandrosterone-Sulfate Coat and Claws Concentrations in Female Dogs from Mating to 60 Days Post-Partum

Fusi

Peric

Probo

et al. 2021

Animals

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In dogs, the phase from mating to the end of weaning lasts about 120 days and encompasses many aspects that, interacting, contribute to increase the allostatic load. The coat and claws, useful for long-term change assessments, have the advantage of being collectable without invasiveness. In the present study, the Cortisol (C) and Dehydroepiandrosterone-sulfate (DHEA-S) concentration monthly changes in the coat and claws were studied in female dogs from mating to the end of weaning to assess Hypothalamic–Pituitary–Adrenal (HPA) axis activation during pregnancy and the post-partum period. The results from 15 Dobermann Pinscher female dogs showed a trend of increase of the coat C from mating to 60 days post-partum, with significant changes between mating and parturition-60 days post-partum (p < 0.01) and between the 30-day pregnancy diagnosis (PD) and 30–60 days post-partum (p < 0.05). The claws C trend showed significant increases between mating and 30–60 days post-partum (p < 0.05) and between the PD and 60 days post-partum (p < 0.01). DHEA-S in both matrices showed non-significant changes. The results suggest that maternity could play a pivotal role in the HPA axis activation, with a subsequent chronic secretion of C determining an increase in the allostatic load in the mothers. Neither maternal parity nor litter size played a significant role in the accumulation of C and DHEA-S in both matrices.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

How Stressful Is Maternity? Study about Cortisol and Dehydroepiandrosterone-Sulfate Coat and Claws Concentrations in Female Dogs from Mating to 60 Days Post-Partum

Fusi

Peric

Probo

et al. 2021

Animals

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“…The property of accumulating hormones during a long time-window allows to reduce the number of samplings, respecting animal welfare. Another advantage is the possibility to store samples without specific precautions, wrapped in paper envelopes and kept at room temperature until the analysis, as previously reported [ 20 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…Both cortisol and DHEA were detected in fingernails of adult humans [ 22 ], and DHEA was measured in nails of infants [ 2 ], suggesting its potential role as a biomarker of intrauterine response to maternal stress. Similarly, the availability of non-invasive samples for fetal and neonatal biology investigation is opening the doors to a deeper knowledge about both late intrauterine and early neonatal canine physiology [ 20 , 24 , 25 ]. Moreover, the refinement of non-invasive and simple techniques of investigation is particularly valuable in canine puppies, given their small dimensions.…”

Section: Introductionmentioning

confidence: 99%

Hair and Claw Dehydroepiandrosterone Concentrations in Newborn Puppies Spontaneously Dead within 30 Days of Age

Fusi

Veronesi

Prandi

et al. 2022

Animals

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The latest intrauterine fetal developmental stage and the neonatal period represent the most challenging phases for mammalian offspring. Toward the term of pregnancy, during parturition, and after birth, the hypothalamic–pituitary–adrenal axis (HPA) is a key system regulating several physiologic processes, through the production of cortisol and dehydroepiandrosterone (DHEA). This study was aimed to assess DHEA concentrations in hair and claws of 126 spontaneously dead newborn puppies, classified as premature puppies (PRE-P), stillborn puppies (STILL-P) and puppies that died from the 1st to the 30th day of life (NEON-P). The possible influence of newborn sex, breed body size, and timing of death on DHEA concentrations in both matrices was evaluated. Claw DHEA concentrations were higher in the PRE-P group when compared to STILL-P and NEON-P puppies (p < 0.05), whilst no significant differences were found in hair for all the studied factors. The results confirm the hypothesis that higher amounts of DHEA are produced during the intrauterine life in dogs, also in puppies that will die soon after birth.

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“…wurden nicht invasive Steroidmessungen auch in Hautanhangsgebilden wie Haaren[22][23], Federn[24][25] oder Krallen[26][27] beschrieben. Die nicht invasive Steroidanalytik ist im Vergleich zur Messung im Blut aus technischer Sicht meist deutlich anspruchsvoller.…”

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Bestimmung von Sexualsteroiden in abgestoßener Haut der Gila-Krustenechse (Heloderma suspectum)

Schuler

Feller²,

Schwandt³

2020

Tierarztl Prax Ausg K Kleintiere Heimtiere

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Zusammenfassung Gegenstand und Ziel Die Messung in Hautanhangsgebilden wie Haaren oder Klauen bei Säugern oder in Federn bei Vögeln wird zur nicht invasiven Bestimmung von Steroidhormonen angewendet. Ziel dieser Arbeit war, in einer Pilotstudie an der Gila-Krustenechse zu testen, ob die Messung von Sexualsteroiden in abgeschilferter Haut bei Reptilien zur Erfassung der endokrinen Gonadenfunktion bzw. zur Geschlechtsbestimmung eingesetzt werden kann. Material und Methoden Für die Untersuchungen standen abgestoßene Häute von 11 weiblichen und 7 männlichen adulten, geschlechtsreifen Tieren zur Verfügung. Große Hautstücke wurden zunächst mit einer Schere zerkleinert, unter flüssigem Stickstoff fein zermörsert und die Proben schließlich mit organischen Lösungsmitteln extrahiert. Die folgenden Parameter wurden in den getrockneten und rückgelösten Extrakten radioimmunologisch bestimmt: Progesteron (P4), Estradiol-17β (E2), Testosteron (T), freie Gesamtöstrogene (fGÖ) sowie freie plus konjugierte Gesamtöstrogene (fkGÖ). Ergebnisse Für P4 (p = 0,0052) und E2 (p = 0,0079) wurden signifikante Geschlechtsunterschiede mit höheren Konzentrationen bei weiblichen im Vergleich zu männlichen Tieren gefunden. Unerwarteterweise ergaben sich bei weiblichen Tieren auch signifikant (p = 0,0232) höhere T-Messwerte als bei männlichen, wobei die Konzentrationen insgesamt nur geringfügig über der Nachweisgrenze lagen. Im Vergleich zu fGÖ waren die Konzentrationen an fkGÖ nur geringgradig höher und zwischen den beiden Geschlechtern bestanden keine signifikanten Unterschiede. Schlussfolgerung und klinische Relevanz Auch wenn die in dieser Pilotstudie angewendeten Methoden aufgrund von Überlappungen zwischen den Geschlechtern weder allein noch in Kombination eine zuverlässige Geschlechtsbestimmung bei einzelnen Tieren erlaubten, könnte die Messung von Sexualsteroiden in abgestoßenen Häuten grundsätzlich eine nützliche Methode für die nicht invasive Geschlechtsbestimmung oder die Erfassung der endokrinen Gonadenfunktion bei bestimmten Reptilienarten sein.

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Perinatal concentrations of 17β-estradiol and testosterone in the toe claws of female and male dogs from birth until 60 days of age

Cited by 5 publications

References 15 publications

How Stressful Is Maternity? Study about Cortisol and Dehydroepiandrosterone-Sulfate Coat and Claws Concentrations in Female Dogs from Mating to 60 Days Post-Partum

How Stressful Is Maternity? Study about Cortisol and Dehydroepiandrosterone-Sulfate Coat and Claws Concentrations in Female Dogs from Mating to 60 Days Post-Partum

Hair and Claw Dehydroepiandrosterone Concentrations in Newborn Puppies Spontaneously Dead within 30 Days of Age

Bestimmung von Sexualsteroiden in abgestoßener Haut der Gila-Krustenechse (Heloderma suspectum)

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