Perinatal exposure to a dietary pesticide cocktail does not increase susceptibility to high-fat diet-induced metabolic perturbations at adulthood but modifies urinary and fecal metabolic fingerprints in C57Bl6/J mice

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

“…During this phase, food (mg/day) and water (ml/day) intake were closely monitored, and no significant differences were observed among the two groups (Table 1). This protocol was previously used by our research consortium (Smith et al, 2020). At weaning, all offspring were fed using standard control pellet and animals were kept in cages of 4 individuals.…”

“…At weaning, all offspring were fed using standard control pellet and animals were kept in cages of 4 individuals. The generation of the chow pellet containing pesticides is identical to the one we have previously described and used in (Klement et al, 2020;Lukowicz et al, 2018;Smith et al, 2020). The selected cocktail contains fungicides and insecticides (ziram, thiophanate, captan, chlorpyrifos, boscalid, and thiacloprid) that are relevant to agriculture, as suggested by the European Food Safety Authority reports (EFSA, 2018;Klement et al, 2020;Lukowicz et al, 2018;Smith et al, 2020).…”

“…Here, by using two distinct mouse and zebrafish models we provide evidence for an impact of maternal or developmental exposure to dietary and environmental pesticides on neuronal functions. In one model, C57BL6/j mice were fed immediately prior to and during mating, during pregnancy and lactation with chow pellets containing a cocktail of six pesticides, with relevance to agriculture (EFSA, 2018; Klement et al, 2020;Lukowicz et al, 2018;Smith et al, 2020) and exposing mice to the TDI for each pesticide. Using male and female offspring, we tracked behavior and in vivo brain electrophysiological outcomes.…”

Toxicology and Microbiota: How Do Pesticides Influence Gut Microbiota? A Review

“…Lukowicz et al aimed to assess the toxicity of a mixture of six pesticides (boscalid, captan, chlorpyrifos, thiophanate, thiacloprid, and ziram) in mice, suggesting that pesticide treatment could affect the regular gut microbiota composition and the host metabolic homeostasis [ 110 ]. The perinatal exposure to a low-dose pesticide cocktail made up of six pesticides (chlorpyrifos, thiacloprid, thiophanate, captan, ziram and boscalid) administered to mice resulted in alterations in the urinary and faecal metabolite due to the alterations in the gut microbial communities [ 111 ].…”

Short-Term and Long-Term Carcinogenic Effects of Food Contaminants (4-Hydroxynonenal and Pesticides) on Colorectal Human Cells: Involvement of Genotoxic and Non-Genomic Mechanisms

“…However, the scientific literature does not cover the areas of multiple pesticide exposure and health consequences. In vivo studies on mixtures of pesticides have shown that dietary exposure may promote the occurrence of chronic diseases, but no mechanistic results are available to date [29,30]. The occurrence of CRC results from the interplay of genetic, environmental and microenvironmental factors [31].…”