Perinatal expression of adenylyl cyclase subtypes in rat brown adipose tissue

Chaudhry, Archana; Muffler, Laurie A.; Yao, Ruihong; Granneman, James G.

doi:10.1152/ajpregu.1996.270.4.r755

Cited by 11 publications

(8 citation statements)

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“…Of the nine mammalian AC isoforms identified, three have been shown to be calmodulinsensitive: AC1, -3, and -8 (11). AC1 and -8 are expressed mainly in tissues of the central nervous system, whereas AC3 has a broader profile, having been found in olfactory neuroepithelium (12), testes (13), brown adipose tissue (14), and uterus (15). Our studies demonstrated the presence of a single CaMsensitive adenylyl cyclase isoform in IMCD, namely AC3.…”

mentioning

confidence: 57%

Calmodulin Is Required for Vasopressin-stimulated Increase in Cyclic AMP Production in Inner Medullary Collecting Duct

Hoffert

Chou

Fenton

et al. 2005

Journal of Biological Chemistry

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Calmodulin plays a critical role in regulation of renal collecting duct water permeability by vasopressin. However, specific targets for calmodulin action have not been thoroughly addressed. In the present study, we investigated whether Ca 2؉ /calmodulin regulates adenylyl cyclase activity in the renal inner medullary collecting duct. Rat inner medullary collecting duct suspensions were incubated in the presence or absence of 0.1 nM vasopressin and the calmodulin inhibitors, monodansylcadaverine, W-7, and trifluoperazine, followed by measurement of cAMP. Vasopressin-stimulated cAMP elevation was significantly attenuated in the presence of calmodulin inhibitors. Analysis of transglutaminase 2 knock-out mice confirmed that these compounds were not acting through inhibition of transglutaminase 2 activity. Calmodulin inhibitors also blocked both cholera toxin-and forskolinstimulated cAMP accumulation. In isolated perfused tubules, W-7 reversibly blocked vasopressin-stimulated urea permeability, a process that requires a rise in intracellular cAMP but does not appear to involve protein trafficking to the apical plasma membrane. These results suggest that calmodulin is required for vasopressinstimulated adenylyl cyclase activity in the intact inner medullary collecting duct. Reverse transcription-PCR, immunoblotting, and immunohistochemistry revealed the presence of the calmodulin-sensitive adenylyl cyclase type 3 in the rat collecting duct, an isoform previously not known to be expressed in the collecting duct. Long-term treatment of Brattleboro rats with a vasopressin analog markedly decreased adenylyl cyclase type 3 protein abundance, providing an explanation for long-term down-regulation of vasopressin response in the collecting duct. These studies demonstrate the importance of calmodulin in the regulation of collecting duct adenylyl cyclase activity and transport function.

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mentioning

confidence: 57%

Calmodulin Is Required for Vasopressin-stimulated Increase in Cyclic AMP Production in Inner Medullary Collecting Duct

Hoffert

Chou

Fenton

et al. 2005

Journal of Biological Chemistry

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“…The differing results for the effects of 5HT receptor activation on basal AC and on forskolin-stimulated AC thus suggested to us that the unique of effects of the OBX lesion in the older animals reflected alterations in AC itself, either in the isoform being expressed or in the association between AC and G-proteins (Chaudhry et al, 1996;Gao et al, 1998Gao et al, , 1999Scarpace et al, 1996;Zeiders et al, 1999b), which is influenced by factors such as phosphorylation (Premont et al, 1992). This explanation was then verified by assessing the ratio of AC responses to forskolin vs Mn 2 þ , two stimulants that act at different AC epitopes and whose relative activities therefore reflect the AC isoform and the effectiveness of G-protein association with AC (Zeiders et al, 1999b).…”

Section: Figurementioning

confidence: 94%

Serotonergic Cell Signaling in an Animal Model of Aging and Depression: Olfactory Bulbectomy Elicits Different Adaptations in Brain Regions of Young Adult vs Aging Rats

Slotkin

Cousins

Tate

et al. 2004

Neuropsychopharmacol

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Aging involves neuronal and synaptic loss, and maintenance of function depends on adaptations in cellular responsiveness. We studied olfactory bulbectomy (OBX), a model that recapitulates monoaminergic dysfunction in depression, in 10-week vs 19-month-old rats, and evaluated 5HT (5-hydroxytryptamine, serotonin) mechanisms. OBX elicited little change in 5HT1A receptors in the cerebral cortex or striatum of either age group. In contrast, 5HT2 receptors showed disparate effects, with a decrease in the cerebral cortex of young OBX but not aging OBX rats, whereas the latter group showed a selective decrease in striatal 5HT2 receptors. Greater differences were apparent for 5HT-mediated cell signaling, assessed for the adenylyl cyclase (AC) cascade. In young animals, 5HT had a stimulatory effect on AC that was unaltered by OBX. However, in aging animals, the pattern of 5HT responses showed marked alterations in response to OBX: under basal conditions, stimulatory effects were enhanced but when AC was activated with forskolin, 5HT became markedly inhibitory in the striatum of aged OBX animals. Assessment of the relative AC responses to two direct stimulants that act on different epitopes of the enzyme, forskolin and Mn2+, pointed to a shift in the AC isoform and/or its ability to associate with G-proteins as the mechanism underlying the age-related differences for OBX effects. These data indicate that there are biological distinctions in the response of 5HT systems to OBX in young adult vs aging animals, which, if present in geriatric depression, could provide a mechanistic basis for differences in responses to antidepressants that act on 5HT.

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“…This suggests that, in sensory neurons, the expression of these genes is coordinated and involves tissue-specific transcription factors. AC3 has also been found expressed in many other tissues, including bovine adipose tissue (20,58), male germ cells (35,57), and luteal cells from bovine ovaries (100). Expression of AC3 mRNA has also been detected in human islets isolated from nondiabetic individuals (193).…”

Section: Transcriptional Regulation Of Ac Genesmentioning

confidence: 99%

Tissue specificity and physiological relevance of various isoforms of adenylyl cyclase

Defer

Best‐Belpomme

Hanoune

2000

American Journal of Physiology-Renal Physiology

358

322

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The present review focuses on the potential physiological regulations involving different isoforms of adenylyl cyclase (AC), the enzymatic activity responsible for the synthesis of cAMP from ATP. Depending on the properties and the relative level of the isoforms expressed in a tissue or a cell type at a specific time, extracellular signals received by the G protein-coupled receptors can be differently integrated. We report here on various aspects of such regulations, emphasizing the role of Ca(2+)/calmodulin in activating AC1 and AC8 in the central nervous system, the potential inhibitory effect of Ca(2+) on AC5 and AC6, and the changes in the expression pattern of the isoforms during development. A particular emphasis is given to the role of cAMP during drug dependence. Present experimental limitations are also underlined (pitfalls in the interpretation of cellular transfection, scarcity of the invalidation models, and so on).

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Perinatal expression of adenylyl cyclase subtypes in rat brown adipose tissue

Cited by 11 publications

References 0 publications

Calmodulin Is Required for Vasopressin-stimulated Increase in Cyclic AMP Production in Inner Medullary Collecting Duct

Calmodulin Is Required for Vasopressin-stimulated Increase in Cyclic AMP Production in Inner Medullary Collecting Duct

Serotonergic Cell Signaling in an Animal Model of Aging and Depression: Olfactory Bulbectomy Elicits Different Adaptations in Brain Regions of Young Adult vs Aging Rats

Tissue specificity and physiological relevance of various isoforms of adenylyl cyclase

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