Perinatal high fat diet exposure alters oxytocin and corticotropin releasing factor inputs onto vagal neurocircuits controlling gastric motility

Carson, Kaitlin E.; Alvarez, Jared; Mackley, Jasmine; Browning, Kirsteen N.

doi:10.1152/physiol.2023.38.s1.5733077

Cited by 1 publication

(2 citation statements)

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“…Receptors for CRH, OXT, PENK, and SST were present, as well as the receptor for TRH to a lesser extent. This receptor expression profile is consistent with literature on PVN‐DMV neurons, which reports the expression of AVP, OXT, PENK, SST, and CRH in preparasympathetic neurons 8,49 . The presence of TRH receptor might indicate relevance for this neuropeptide in PVN‐DMV circuitry as well, though TRH projections could also originate from brain regions other than the PVN.…”

Section: Discussionsupporting

confidence: 91%

“…This receptor expression profile is consistent with literature on PVN-DMV neurons, which reports the expression of AVP, OXT, PENK, SST, and CRH in preparasympathetic neurons. 8,49 The presence of TRH receptor might indicate relevance for this neuropeptide in PVN-DMV circuitry as well, though TRH projections could also originate from brain regions other than the PVN. As the DMV-projecting AVP and OXT neurons were reportedly parvocellular, we can infer that the Avp-Adarb2 and Oxt-Adarb2 populations are the most likely candidates for the vasopressinergic and oxytocinergic PVN-DMV connections.…”

Section: Discussionmentioning

confidence: 99%

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An integrated single‐cell RNA‐seq atlas of the mouse hypothalamic paraventricular nucleus links transcriptomic and functional types

Berkhout,

Poormoghadam,

et al. 2024

J Neuroendocrinology

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The hypothalamic paraventricular nucleus (PVN) is a highly complex brain region that is crucial for homeostatic regulation through neuroendocrine signaling, outflow of the autonomic nervous system, and projections to other brain areas. In the past years, single‐cell datasets of the hypothalamus have contributed immensely to the current understanding of the diverse hypothalamic cellular composition. While the PVN has been adequately classified functionally, its molecular classification is currently still insufficient. To address this, we created a detailed atlas of PVN transcriptomic cell types by integrating various PVN single‐cell datasets into a recently published hypothalamus single‐cell transcriptome atlas. Furthermore, we functionally profiled transcriptomic cell types, based on relevant literature, existing retrograde tracing data, and existing single‐cell data of a PVN‐projection target region. Finally, we validated our findings with immunofluorescent stainings. In our PVN atlas dataset, we identify the well‐known different neuropeptide types, each composed of multiple novel subtypes. We identify Avp‐Tac1, Avp‐Th, Oxt‐Foxp1, Crh‐Nr3c1, and Trh‐Nfib as the most important neuroendocrine subtypes based on markers described in literature. To characterize the preautonomic functional population, we integrated a single‐cell retrograde tracing study of spinally projecting preautonomic neurons into our PVN atlas. We identify these (presympathetic) neurons to cocluster with the Adarb2+ clusters in our dataset. Further, we identify the expression of receptors for Crh, Oxt, Penk, Sst, and Trh in the dorsal motor nucleus of the vagus, a key region that the pre‐parasympathetic PVN neurons project to. Finally, we identify Trh‐Ucn3 and Brs3‐Adarb2 as some centrally projecting populations. In conclusion, our study presents a detailed overview of the transcriptomic cell types of the murine PVN and provides a first attempt to resolve functionality for the identified populations.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%