Periocular and Intra-Articular Injection of Canine Adipose-Derived Mesenchymal Stem Cells: An <i>In Vivo</i> Imaging and Migration Study

Wood, Joshua A.; Chung, Dai Jung; Park, Shin Ae; Zwingenberger, Allison L.; Reilly, Christopher M.; Ly, Irene; Walker, Naomi J.; Vernau, William; Hayashi, Kei; Wisner, Erik R.; Cannon, Matthew S.; Kass, Philip H.; Cherry, Simon R.; Borjesson, Dori L.; Russell, Paul; Murphy, Christopher J.

doi:10.1089/jop.2011.0166

Cited by 49 publications

(43 citation statements)

References 52 publications

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“…MSCs were isolated from fat and cultured exactly as previously described for canine MSCs [21][22][23]. MSCs were cultured in low-glucose Dulbecco's modified Eagle's medium (Mediatech), 10% fetal bovine serum (HyClone, Inc.), and 1% penicillin/streptomycin (Thermo Fisher Scientific) in tissue culture flasks (Nunc) and incubated at 37°C, 5% CO 2 .…”

Section: Fmsc Isolation and Expansionmentioning

confidence: 99%

Feline Foamy Virus Adversely Affects Feline Mesenchymal Stem Cell Culture and Expansion: Implications for Animal Model Development

Arzi

Kol

Murphy

et al. 2015

Stem Cells and Development

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Mesenchymal stem cells (MSCs) are a promising therapeutic option for various immune-mediated and inflammatory disorders due to their potent immunomodulatory and trophic properties. Naturally occurring diseases in large animal species may serve as surrogate animal models of human disease, as they may better reflect the complex genetic, environmental, and physiologic variation present in outbred populations. We work with naturally occurring diseases in large animal species to better understand how MSCs work and to facilitate optimal translation of MSC-based therapies. We are investigating the use of MSC therapy for a chronic oral inflammatory disease in cats. During our efforts to expand fat-derived feline MSCs (fMSCs), we observed that*50% of the cell lines developed giant foamy multinucleated cells in later passages. These morphologic alterations were associated with proliferation arrest. We hypothesized that the cytopathic effects were caused by infection with a retrovirus, feline foamy virus (FFV). Using transmission electron microscopy, polymerase chain reaction, and in vitro assays, we determined that syncytial cell formation and proliferation arrest in fMSCs were caused by FFV strains that were highly homologous to previously reported FFV strains. We determined that the antiretroviral drug, tenofovir, may be used to support ex vivo expansion and salvage of FFV-infected fMSC lines. MSC lines derived from specific pathogen-free cats do not appear to be infected with FFV and may be a source of allogeneic fMSCs for clinical application. FFV infection of fMSC lines may hinder large-scale expansion of autologous MSC for therapeutic use in feline patients.

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Section: Fmsc Isolation and Expansionmentioning

confidence: 99%

Feline Foamy Virus Adversely Affects Feline Mesenchymal Stem Cell Culture and Expansion: Implications for Animal Model Development

Arzi

Kol

Murphy

et al. 2015

Stem Cells and Development

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“…MSCs were isolated from fat and cultured exactly as previously described [8,30]. MSCs were cultured in lowglucose Dulbecco's modified Eagle's medium (DMEM; Mediatech, Manassas, VA), 10% fetal bovine serum (FBS; HyClone, Inc., Logan, UT), and 1% penicillin/streptomycin (P/S; Invitrogen, Carlsbad, CA) in tissue culture flasks (Nunc, Roskilde, Denmark) and incubated at 37°C with 5% CO 2 .…”

Section: Msc Culturementioning

confidence: 99%

“…MSCs are in phase I-III clinical trials for the treatment of immune-mediated and inflammatory intestinal diseases, such as graft-versus-host disease and inflammatory bowel disease in people [5][6][7][8]. The therapeutic rationale is based on the ability of MSCs to home to and engraft in the lamina propria of the gastrointestinal (GI) tract during intestinal inflammation and exert potent immunomodulatory functions.…”

Section: Introductionmentioning

confidence: 99%

Gastrointestinal Microbes Interact with Canine Adipose-Derived Mesenchymal Stem Cells In Vitro and Enhance Immunomodulatory Functions

Kol

Foutouhi

Walker

et al. 2014

Stem Cells and Development

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Mesenchymal stem cells (MSCs) are somatic, multipotent stromal cells with potent immunomodulatory and regenerative properties. Although MSCs have pattern recognition receptors and are modulated by Toll-like receptor ligands, MSC-microbial interactions are poorly defined. The objectives of this study were to determine the effect of bacterial association on MSC function. We hypothesized that gastrointestinal bacteria associate with MSCs and alter their immunomodulatory properties. The effect of MSC-microbial interactions on MSC morphology, viability, proliferation, migration, and immunomodulatory functions was investigated. MSCs associated with a remarkable array of enteric pathogens and commensal bacteria. MSC interactions with two model organisms, the pathogen Salmonella typhimurium and the probiotic Lactobacillus acidophilus, were further investigated. While ST readily invaded MSCs, LB adhered to the MSC plasma membrane. Neither microbe induced MSC death, degeneration, or diminished proliferation. Microbial association did not upregulate MHC-II, CD80/86, or CD1 expression. MSCmicrobial interaction significantly increased transcription of key immunomodulatory genes, including COX2, IL6, and IL8, coupled with significantly increased prostaglandin E 2 (PGE 2 ), interleukin (IL)6, and IL8 secretion. MSC-ST coincubation resulted in increased MSC expression of CD54, and significant augmentation of MSC inhibition of mitogen-induced T-cell proliferation. T-cell proliferation was partially restored when PGE 2 secretion was blocked from ST-primed MSCs. MSC-microbe interactions have a profound effect on MSC function and may be pivotal in a variety of clinical settings where MSCs are being explored as potential therapeutics in the context of microbial communities, such as Crohn's disease, chronic nonhealing wounds, and sepsis.

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“…Wood ve ark tarafindan yapılan çalışmada kök hücreler sistemik dolaşıma verilmiş ve takip edilmiştir. Hücrelerin timus ve gastrointestinal kanal lokalizasyonu belirlenmiştir (26). MKH'lerin hasarlı dokuya doğru mobilizasyonunu, hasarlı dokunun değişen mikroçevresinden gelen uyarıların sağladığı gösterilmiştir.…”

Section: Mkh' Lerin Veteriner Hekimlikte Kullanımıunclassified

Mezenkimal kök hücreler ve veteriner hekimlikte kullanımı

SANCAK¹

2014

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Kök hücreler organizmada farklı hücre tiplerine dönüşebilen, canlı kaldıkça yaşamlarını sürdürme özelliğine sahip ve kendi kendilerini yenileyebilen hücrelerdir. Elde edildiği yere göre embriyonal ve yetişkin kök hücresi olmak üzere 2 tip kök hücre bulunmaktadır. Blastositin iç hücre kitlesinden elde edilen embriyonal kök hücreler tüm canlıyı oluşturabilirken differensiye olmuş bir dokudan elde edilen kök hücre sınırlı bölünme ve farklılaşma potansiyeline sahiptir. Embriyonal kök hücreler ile ilgili etik sınırlamalardan dolayı çalışmalar kısıtlıdır. Mezenkimal kök hücrelerdeki çalışmalar hem elde ediliş kolaylığı hem de rejeneratif tıpta kullanım alanı bulması ile öne çıkmaktadır. İnsanlarda kök hücre çalışmaları lokalize hastalıklarda, sistemik transplantasyonda, gen tedavisi ile kombine kök hücre tedavisinde ve doku mühendisliğinde olmak üzere dört alanda başlamıştır. Veteriner hekimlikte kök hücre çalışmaları insan hastalıklarına model oluşturması açısından deney hayvanlarında başlamıştır. Klinik olarak atlarda tendo yaralanmalarında köpeklerde ise osteoartiritisin/kıkırdak hasarının tedavisinde kullanım alanı bulmaktadır.Anahtar sözcükler: Kemik iliği, klinik uygulama, kök hücre. Mesenchymal stem cells (Msc) and stem cell applications in veterinary medicineSummary: In the living organism stem cells differentiate into diverse specialized cell types, capable of sustaining viability and self-renewal. Stem cells can be categorized as embryonal, and adult due to its origin. Embryonic stem cells obtained from the inner cell mass of the blastocyst has the ability to create the whole organism, differentiated tissue-derived stem cells have the limited growth and differentiation potential and tend to turn into the tissue from which they originated. Due to ethical limitations studies in the embryonic stem cells are limited. Ease of obtaining and common use of the cells in regenerative medicine makes mesenchymal stem cells favorable for studies. In humans; localized disease, systemic transplantations, stem cell therapy in combination with gene therapy and tissue engineering are the four areas of work to be started on stem cells. Stem cell research in veterinary medicine started in experimental animals in terms of being a good model for human diseases. Clinically mesenchymal stem cells are broadly used in horses for tendon injuries and in dogs for osteoartiritis/ injury of the cartilage.

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Periocular and Intra-Articular Injection of Canine Adipose-Derived Mesenchymal Stem Cells: An In Vivo Imaging and Migration Study

Abstract: The active engraftment of the MSCs in combination with their previously documented immunomodulatory capabilities suggests the potential for therapeutic benefit in using MSCs for the treatment of periocular and joint diseases with immune involvement.

Cited by 49 publications

References 52 publications

Feline Foamy Virus Adversely Affects Feline Mesenchymal Stem Cell Culture and Expansion: Implications for Animal Model Development

Feline Foamy Virus Adversely Affects Feline Mesenchymal Stem Cell Culture and Expansion: Implications for Animal Model Development

Gastrointestinal Microbes Interact with Canine Adipose-Derived Mesenchymal Stem Cells In Vitro and Enhance Immunomodulatory Functions

Mezenkimal kök hücreler ve veteriner hekimlikte kullanımı

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